Immunocytochemical utility of claudin‐4 versus those of Ber‐EP4 and MOC‐31 in effusion cytology

Oda, Tomohiro; Ogata, Sho; Kawaguchi, Saki; Minabe, Shinya; Dokyu, Misaki; Takahashi, Hiromi; Kumazawa, Fumihiko; Shimazaki, Hideyuki; Takano, Masashi; Hase, Kazuo; Ozeki, Yuichi; Kanoh, Soichiro; Nakanishi, Kuniaki

doi:10.1002/dc.23476

Cited by 25 publications

(33 citation statements)

References 19 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Claudin 4 shows improved sensitivity and specificity over Ber-EP4 and MOC-31 for distinguishing adenocarcinoma from cells of mesothelial origin. [13][14][15] Although Ber-EP4 and MOC-31 are reasonably sensitive as adenocarcinoma markers, both demonstrate positive staining in a subset of epithelioid mesotheliomas, which represents a potential diagnostic pitfall in effusion specimens. 14,15 A study found no membranous staining in 75 malignant mesotheliomas, although 11% (8 of 75 cases) had weak cytoplasmic staining, which is considered nonspecific.…”

Section: Claudinmentioning

confidence: 99%

Ancillary studies in pleural, pericardial, and peritoneal effusion cytology

Sundling¹,

Cibas

2018

Cancer Cytopathology

View full text Add to dashboard Cite

Pleural, pericardial, and peritoneal effusion specimens present diagnostic challenges and clinical opportunities for cytology. For the patient, these specimens may be the first diagnosis of malignancy or the first sign of disease recurrence. This review aims to provide useful approaches with which to resolve key difficulties in cytologic diagnosis through the use of ancillary studies, focusing on immunohistochemistry. These approaches are suggested in concert with clinical, radiographic, and morphologic findings. The differentiation of reactive mesothelial cells from malignant mesothelioma and adenocarcinoma is a recurring theme, and Wilms tumor 1 (WT1)/AE1/AE3, claudin 4, and BRCA1-associated protein 1 (BAP1) immunostains are useful new tools in the armamentarium. A targeted workup is suggested for patients with no known primary site or with multiple prior malignancies. Molecular and other biomarker testing can be performed on even modestly cellular fluid specimens and may allow patients to benefit from targeted therapy without the need for additional tissue biopsies. Cancer Cytopathol 2018;000:000-000. © 2018 American Cancer Society.

show abstract

Section: Claudinmentioning

confidence: 99%

Ancillary studies in pleural, pericardial, and peritoneal effusion cytology

Sundling¹,

Cibas

2018

Cancer Cytopathology

View full text Add to dashboard Cite

show abstract

“…Immunostains that can distinguish between mesothelial, both reactive or neoplastic, and epithelial cells in effusions continue to be evaluated in human pathology . Such a comprehensive evaluation has never been performed in the veterinary field, although its importance is well recognized .…”

Section: Introductionmentioning

confidence: 99%

The cell tube block technique and an immunohistochemistry panel including Wilms tumor 1 to assist in diagnosing cavitary effusions in dogs and cats

Marcos

Marrinhas

Malhão

et al. 2019

Veterinary Clinical Pathol

View full text Add to dashboard Cite

Background: Cell blocks and immunohistochemistry (IHC) are increasingly recognized as being complementary tools for cytologic diagnostics, especially for neoplastic diseases. Objectives:The study aimed to evaluate the utility of cell tube block (CTB) IHC for refining the diagnosis of effusions in dogs and cats.Methods: Cavitary effusions (n = 25) from dogs and cats classified by cytology as reactive, neoplastic, borderline (suspicious of neoplasia), and chylous were studied.CTB sections were stained with H&E, and immunostained with PAX-5, CD3, pancytokeratin (CK), vimentin, and Wilms tumor 1 protein (WT1) antibodies, according to the cytologic diagnoses. A histologic case series of confirmed normal, reactive, and neoplastic mesothelium and several different carcinomas were included to test the utility of WT1 as a marker of mesothelial cells. Results:CTBs had a layered appearance with reduced background staining. CD3 and PAX5 immunolabeling allowed immunophenotype assessment in all of the lymphoma cases. In carcinomatous effusions, neoplastic cells were CK-positive, WT1negative, and vimentin-negative (except for two cases). Wilms tumor 1 protein was positive in the nuclei of normal, reactive, and neoplastic mesothelial cells, and ovarian carcinomatous cells. Other carcinomas and lymphomas were negative. Conclusions:CTBs are valuable tools to assist in making a diagnosis of cavitary effusions in dogs and cats, and WT1 is a promising marker to differentiate mesothelial from carcinomatous cells. K E Y W O R D Scell blocks, cytology, effusion, immunohistochemistry, mesothelial cells

show abstract

“…The sensitivity for metastatic carcinoma detection from effusion/peritoneal washing was 100, 97.64 and 88.23% for claudin-4, MOC-31 and p16, respectively. Claudin-4 was used as a reference for comparison with the p16 results since it is considered as the most sensitive marker to distinguish adenocarcinomas from reactive and malignant mesothelial cells in cytology of effusions [18,19]. MOC-31 is also a useful diagnostic marker with high sensitivity and specificity for differentiating malignant from benign effusions.…”

Section: Discussionmentioning

confidence: 99%

“…MOC-31 is also a useful diagnostic marker with high sensitivity and specificity for differentiating malignant from benign effusions. However, this marker may not be expressed in some carcinomas of histological types other than adenocarcinomas, which may result in false negative diagnoses [18].…”

Section: Discussionmentioning

confidence: 99%

P16 as a marker of carcinoma in effusions and peritoneal washing

Carneiro¹,

Amorim²,

Carneiro³

et al. 2020

BMC Cancer

View full text Add to dashboard Cite

Background: Considering the potential of p16 as a marker for diagnosis, prognosis and therapeutic response, the aim of this study was to assess its presence, via immunocytochemistry, in metastatic carcinoma of different primary sites and histological types obtained from effusions and peritoneal washings. A total of 118 samples including 85 of metastatic carcinoma and 33 samples of benign effusion/peritoneal washing were prepared by the plasma/ thromboplastin method. Immunocytochemistry reactions were performed on cell block sections using antibodies against p16, claudin-4, MOC-31, calretinin, HBME and CD68. Results: P16 overexpression was observed in 88.23% of all carcinoma samples. All cervix adenocarcinoma samples showed p16 overexpression. Overexpression in adenocarcinomas of ovary, lung and breast was observed in 93.75, 93.10 and 75% of the samples, respectively. Overexpression was observed in all different histological types analyzed: small cell carcinoma (lung), squamous cell carcinoma (cervical) and urothelial carcinoma (bladder). The specificity of p16 for carcinoma detection was of 96.96%.Conclusion: Overexpression of p16 was observed in most metastatic carcinoma, from different primary sites and histological types, obtained from effusions and peritoneal washings. Due to its high frequency of overexpression in metastatic carcinoma, p16 may play a possible role in tumor progression and it may be considered as a complementary diagnostic marker depending on histological type and primary site of carcinoma.

show abstract

Immunocytochemical utility of claudin‐4 versus those of Ber‐EP4 and MOC‐31 in effusion cytology

Abstract: Since CL4 exhibited similar or superior usefulness to Ber-EP4 and MOC-31, it could become the first choice for the above differential diagnosis in effusion cytology. Diagn. Cytopathol. 2016;44:499-504. © 2016 Wiley Periodicals, Inc.

Cited by 25 publications

References 19 publications

Ancillary studies in pleural, pericardial, and peritoneal effusion cytology

Ancillary studies in pleural, pericardial, and peritoneal effusion cytology

The cell tube block technique and an immunohistochemistry panel including Wilms tumor 1 to assist in diagnosing cavitary effusions in dogs and cats

P16 as a marker of carcinoma in effusions and peritoneal washing

Contact Info

Product

Resources

About