P16 as a marker of carcinoma in effusions and peritoneal washing

Carneiro, Fabiana Pirani; Amorim, Rivadávio Fernandes Batista de; Carneiro, Marcos de Vasconcelos; Castro, Tércia Maria Mendes Lousa de; Vianna, Leonora Maciel de Souza; Takano, Gustavo Henrique Soares; Darós, Andersen Charles; Peres, Isabela; Kückelhaus, Selma Aparecida Souza; Motoyama, Akira

doi:10.1186/s12885-020-6670-5

Cited by 3 publications

(3 citation statements)

References 24 publications

(33 reference statements)

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“…Regarding the scoring intensity of p16 expression in grade III canine osteosarcomas, our results revealed a strong positive nuclear staining intensity in 66% of the osteoblast cancer cells. Recently, it has been shown that increase expression of p16 is correlated with most metastatic carcinoma (26). Therefore, p16 can be contemplated as a diagnostic marker in grade III canine osteosarcomas.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of proliferation markers in canine osteosarcoma

Al-Jameel¹,

Al-Sabaawy²,

Abed³

et al. 2022

IJVS

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Canine osteosarcoma is an extremely malignant bone tumor that often arises in the bones of the limbs. It is a highly metastatic disease distinguished by proliferative bone lesions and a tendency for pulmonary metastasis. Overexpression of proliferative proteins are associated with bad prognosis in human osteosarcoma. Here, we tested the expression of the different proliferative proteins (p53, p16, vimentin, and mdm2) in nine archival samples with canine osteosarcoma. Paraffin-embedded tissue sections were confirmed by histopathology and stained by immunohistochemistry for p53, p16, vimentin and mdm2. Positive expression of these proteins was evaluated as the ratio of positive cancer cells and the intensity staining was assessed in several areas. Histopathologically, 95% of samples were grade II and III. All high-grade osteosarcomas were particularly cellular. The cancer cells were generally large spindle-shaped and large nucleus with distribution of osteoid between the cancer cells. Immunohistochemical detection of p53, p16, vimentin and mdm2 was 89%, 56%, 78%, and 89% of samples respectively. The staining intensity for p53, p16, vimentin and mdm2 was particularly nuclear in 81%, 66%, 78%, and 79% of the cancer cells respectively. Our present work suggests that p53, p16, vimentin, and mdm2 were detected in grade III canine osteosarcomas samples. In addition, these proliferative markers are the significant biomarkers in canine osteosarcomas and can be used as a predictor for diagnostic and prognostic value and allowing cancer differentiation. This primary data supports that both canine and human osteosarcomas share same molecular characters which are approved by expression of proliferative genes.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical expression of proliferation markers in canine osteosarcoma

Al-Jameel¹,

Al-Sabaawy²,

Abed³

et al. 2022

IJVS

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“…20,21 P16 is especially important for cytopathology when the P53 protein is absent in ascites samples with a low number of tumor cells and may lead to wrong assumptions. 22 For this reason, P16 and P53 together are used as key markers for the differential diagnosis of HGSC and LGSC. 2,9 Our analysis of P53/P16 index confirmed 90% concordance between cytopathology and histology.…”

Section: Discussionmentioning

confidence: 99%

“…More than 80% of HGSCs are diffusively positive for P16 20,21 . P16 is especially important for cytopathology when the P53 protein is absent in ascites samples with a low number of tumor cells and may lead to wrong assumptions 22 . For this reason, P16 and P53 together are used as key markers for the differential diagnosis of HGSC and LGSC 2,9 .…”

Section: Discussionmentioning

confidence: 99%

Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high‐grade serous carcinoma from ascites

Miceska

Škof

Novaković

et al. 2022

Cancer Cytopathology

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Background: High-grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of this study was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and BRCA1/2 mutations from ascites. Methods:The study included 45 patients with histologically confirmed primary HGSC and malignant ascites. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki-67 was performed on cytospins and cytoblocks prepared from ascites. Next-generation sequencing (NGS) was used to detect germline/somatic BRCA1/2 mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of primary tumor.Cronbach α and χ 2 statistics, respectively, were used.Results: ICC/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (α > 0.75), whereas poor reliability and significant differences were observed for Ki-67 between ascites and tissue blocks (α < 0.26; p < .001 [Kruskal-Wallis]). For germline BRCA1/2 mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations.Conclusions: These results demonstrate that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline BRCA1/ 2 mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic BRCA1/2 mutations.

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