Immunocytochemical localization of laminin in rat anterior pituitary cells in vivo and in vitro

Tougard, Claude; Louvard, Daniel; Picart, R; Tixier‐Vidal, A.

doi:10.1007/bf02620915

Cited by 37 publications

(26 citation statements)

References 19 publications

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“…6). The production of various ECM proteins, such as laminin, fibronectin, and collagen, by hormone-producing cells in the anterior pituitary has been reported [9,23]. The observations depicted in Figure 6 may be due to the process described as follows: as the anterior pituitary cells form clusters and the ECM is gradually discharged from the cells, integrin including α5 subunit is synthesized, bound to the ECM, and eventually stabilized in the cell membrane.…”

Section: Discussionmentioning

confidence: 91%

An In Vivo and In Vitro Immunohistochemical Study of Integrin Alpha Subunit Localization in the Rat Anterior Pituitary

Kikuchi

Sunaga

Yatabe

et al. 2005

Acta Histochem. Cytochem

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Different types of integrin alpha subunit were investigated immunohistochemically to determine the relationship between the cell adhesion molecule and the local morphology of the rat anterior pituitary using anterior pituitary tissues and primary cultured cells. Alpha 3 subunit was shown to be the main integrin subunit in the anterior pituitary. In vitro, immunoreactions of 3 subunit appeared mainly surrounding cell clusters. The distribution roughly overlapped with immunoreaction of laminin, while it was also observed where expression of laminin was negative. When dispersed cells were primary cultured for 2, 3 or 5 days, immunoreaction of 3 subunit was clearly detected in the vicinity of cell membrane throughout the period; while that of 5 subunit was not observed on day 2, it was observed as dots in cytoplasm on day 3 and more distinctly as lines in the vicinity of the cell membrane on day 5. Alpha 5 subunit was not detectable in vivo. These results indicate the possibility that integrin 3 subunit not only binds the cells to the basement membrane but also to other extracellular matrix or directly to adjacent cells in the anterior pituitary. In addition, integrin 5 subunit may supplement the function of 3 subunit under certain conditions.

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Section: Discussionmentioning

confidence: 91%

An In Vivo and In Vitro Immunohistochemical Study of Integrin Alpha Subunit Localization in the Rat Anterior Pituitary

Kikuchi

Sunaga

Yatabe

et al. 2005

Acta Histochem. Cytochem

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“…In normal adenohypophysis parenchyma laminin is abundantly expressed [46]. Laminin was observed to be expressed in basement membranes and in endocrine cells and nonendocrine folliculo-stellate cells of the rat pituitary [38,40,47] in vivo and in primary cultures. The presence of laminin in the adenohypophysis of lactating female rats was also demonstrated [48].…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 95%

“…Similar distribution was observed in normal human pituitaries. In the pituitary gland of the adult rat laminin immunoreactivity has been found intracellularly within membranous cisternae of endocrine cells of the anterior lobe [40,47]. Labelled secretory granules were observed in gonadotrophs, thyrotrophs, corticotrophs, and lactotrophs; however, not usually in somatotrophs: each glandular cell type seemed to display a specific pattern of the subcellular distribution of laminin immunoreactivity.…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 98%

“…Laminin has been detected intracellularly in lactotrophs [40,47,48]. In the normal pituitary, cells expressing PRL are sited close to laminin-containing cells [46].…”

Section: Lactotrophsmentioning

confidence: 99%

“…Laminin has been detected intracellularly in gonadotrophs of rats [40,47,48], and also in the adult mouse in which laminin immunoreactivity has been detected in gonadotrophs (but not other cell types). Laminin is also a constituent of the basal laminae in the adenohypophysis of the mouse pituitary gland [86].…”

Section: Gonadotrophsmentioning

confidence: 99%

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Extracellular Matrix Proteins in the Anterior Pituitary Gland

Kanasaki¹

2011

TONEUROEJ

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Abstract; The importance of the extracellular matrix (ECM) has become recognised as an important component of the functioning of cells. However the roles of the ECM in the anterior pituitary gland have received little attention. The pituitary has the particular characteristics of possessing a number of endocrine cells as well as less well studied nonendocrine cells. Because each cell has specific and vital roles in the physiology being of an individual the ECM must also possess selective activities. The details of ECM behaviour have not yet been delineated in the pituitary and this review first considers general characteristics of ECM compounds and then examines the properties of peptides with regard to functioning of the cells of the pituitary. It is noted that there are variations between ECM associated with normal cells and with tumours, that there are both morphological and biochemical responses to alterations in the composition of substrate on which cells are grown, and that a development of further models of ECM will be required for continued advances in the understanding of the manner in which the pituitary behaves in normal, clinical and pathological circumstances. Overall, the observations suggest that modulation of the ECM provides opportunities for enhancing well-being of individuals.

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Distribution of laminin in the murine pituitary

et al. 1990

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The distribution and cellular localization of the glycoprotein laminin were investigated by light and electron microscopic immunocytochemistry in the adult murine pituitary gland. Immunoblots confirmed that laminin was the only protein in the pituitary gland of the adult male mouse to react with antilaminin serum. Laminin immunoreactivity was demonstrated at the light microscopic level simultaneously with that of beta-follicle stimulating hormone (beta-FSH) and beta-luteinizing hormone (beta-LH). In addition to its distribution is basal laminae, laminin immunoreactivity was coincidently expressed in gonadotrophs with the immunoreactivities of beta-FSH and beta-LH. Electron microscopic immunocytochemistry was employed on aldehyde-fixed sections embedded in L.R. White. Sites of binding of primary antisera to laminin were identified with affinity-purified secondary antisera directly coupled to 20 nm particles of colloidal gold. Three antisera recognizing laminin were compared and found to result in an identical pattern of immunoreactivity. Laminin was found extracellularly only in formed basal laminae in all three lobes of the pituitary and was not found in extracellular matrices of connective tissue. Laminin immunoreactivity was also found intracellularly in gonadotrophs but in none of the other endocrine or non-endocrine cells of the anterior lobe. Within gonadotrophs, only secretory granules were labeled. The majority, but not all, secretory granules were labeled in each of the gonadotrophs examined, and the proportion of granules labeled with laminin could not be increased by doubling the concentration of anti-laminin serum. Laminin immunoreactivity segregated with the subset of secretory granules containing beta-FSH. In contrast, laminin immunoreactivity was absent in the smaller subset of secretory granules that contain serotonin.(ABSTRACT TRUNCATED AT 250 WORDS)

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Immunocytochemical localization of laminin in rat anterior pituitary cells in vivo and in vitro

Cited by 37 publications

References 19 publications

An In Vivo and In Vitro Immunohistochemical Study of Integrin Alpha Subunit Localization in the Rat Anterior Pituitary

An In Vivo and In Vitro Immunohistochemical Study of Integrin Alpha Subunit Localization in the Rat Anterior Pituitary

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Distribution of laminin in the murine pituitary

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