Distribution of laminin in the murine pituitary

Nunez, Eladio A.; Pomeranz, Howard D.; Gershon, Michael D.; Payette, Robert F.

doi:10.1002/ar.1092260409

Cited by 3 publications

(4 citation statements)

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“…In contrast to other tissues, the specific laminin isoforms expressed in the anterior pituitary have not been identified, although several studies have confirmed the presence of laminin protein in this gland [10, 11, 13, 19]. In the present study, we used RT-PCR to identify laminin chains expressed in the anterior pituitary.…”

Section: Introductionmentioning

confidence: 84%

Laminin Isoforms and Laminin-Producing Cells in Rat Anterior Pituitary

Ramadhani

Tsukada

Fujiwara

et al. 2012

Acta Histochem. Cytochem

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Laminin is a key component of the basement membrane and is involved in the structural scaffold and in cell proliferation and differentiation. Research has identified 19 laminin isoforms, which are assemblies of α, β, and γ chains (eg, the α1, β1, and γ1 chains form the laminin 111 isoform). Although laminin is known to be present in the anterior pituitary, the specific laminin isoforms have not been identified. This study used molecular biological and histochemical techniques—namely, RT-PCR, immunohistochemistry, and in situ hybridization—to identify the laminin isoforms and laminin-producing cells in rat anterior pituitary. RT-PCR showed that laminin α1, α3, and α4 genes were expressed in anterior pituitary. Immunohistochemistry revealed laminin α1 in gonadotrophs and laminin α4 in almost all vascular endothelial cells. Laminin α3 was seen in a subset of vascular endothelial cells. We then performed in situ hybridization to localize β and γ chains in these cells and found that laminin β1, β2, and γ1 were expressed in gonadotrophs and that laminin β1 and γ1 were expressed in endothelial cells. In conclusion, we identified gonadotroph-type (laminin 111 and 121) and vascular-type (laminin 411 and 311) laminin isoforms in rat anterior pituitary.

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Section: Introductionmentioning

confidence: 84%

Laminin Isoforms and Laminin-Producing Cells in Rat Anterior Pituitary

Ramadhani

Tsukada

Fujiwara

et al. 2012

Acta Histochem. Cytochem

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“…Moreover, within these cells, laminin immunoreactivity was found only in secretory granules and not in Golgi cisternae nor in the endoplasmic reticulum or nuclear envelope. Laminin was not found in the extracellular connective tissue [86].…”

Section: Gonadotrophsmentioning

confidence: 79%

“…Laminin is also a constituent of the basal laminae in the adenohypophysis of the mouse pituitary gland [86]. Moreover, within these cells, laminin immunoreactivity was found only in secretory granules and not in Golgi cisternae nor in the endoplasmic reticulum or nuclear envelope.…”

Section: Gonadotrophsmentioning

confidence: 95%

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Kanasaki¹

2011

TONEUROEJ

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Abstract; The importance of the extracellular matrix (ECM) has become recognised as an important component of the functioning of cells. However the roles of the ECM in the anterior pituitary gland have received little attention. The pituitary has the particular characteristics of possessing a number of endocrine cells as well as less well studied nonendocrine cells. Because each cell has specific and vital roles in the physiology being of an individual the ECM must also possess selective activities. The details of ECM behaviour have not yet been delineated in the pituitary and this review first considers general characteristics of ECM compounds and then examines the properties of peptides with regard to functioning of the cells of the pituitary. It is noted that there are variations between ECM associated with normal cells and with tumours, that there are both morphological and biochemical responses to alterations in the composition of substrate on which cells are grown, and that a development of further models of ECM will be required for continued advances in the understanding of the manner in which the pituitary behaves in normal, clinical and pathological circumstances. Overall, the observations suggest that modulation of the ECM provides opportunities for enhancing well-being of individuals.

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“…In addition, immunocytochemical studies have demonstrated the presence of laminin in the secretory granules of rat anterior pituitary (15)(16)(17) and in gonadotrophs in mouse pituitary (18). In these reports, the absence of laminin A chain in the granules was demonstrated by immunoblotting (17,18). The role of laminin in these secretory granules is unknown at present.…”

mentioning

confidence: 99%

Presence of Laminin B Chain-Like Protein in Bovine and Rat Adrenal Chromaffin Granules

Fujino¹,

Fujii²,

Daimon³

1994

The Journal of Biochemistry

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The presence of a glycoprotein laminin in bovine adrenal chromaffin granules was examined by SDS-PAGE followed by immunoblotting. The two chromaffin granule membrane fractions were obtained by linear sucrose gradient centrifugation followed by freezing and thawing and gel-filtration of the chromaffin granule-rich fraction, respectively. The purity of the granules in these fractions was examined by electron microscopy. These fractions contained laminin B chain-like immunoreactivity as a major immunoreactive component against anti-laminin. Laminin A chain-like immunoreactive protein was undetectable. The soluble fraction of the chromaffin granules contained no immunoreactive peptide. The presence of laminin-like immunoreactivity in the chromaffin granules was confirmed by immunocytochemical study. Laminin B chain-like immunoreactivity was also identified in the rat adrenal chromaffin granule fraction. Laminin A chain was hardly detected, as in the case of bovine adrenals. Structure of laminin in chromaffin granules in bovine and rat adrenals may be different from that of mouse Englebrethe-Holm-Swarm sarcoma laminin. The functional significance of laminin B chain-like protein in the granules is unknown at present.

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Distribution of laminin in the murine pituitary

Cited by 3 publications

References 50 publications

Laminin Isoforms and Laminin-Producing Cells in Rat Anterior Pituitary

Laminin Isoforms and Laminin-Producing Cells in Rat Anterior Pituitary

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Presence of Laminin B Chain-Like Protein in Bovine and Rat Adrenal Chromaffin Granules

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