Immunocytochemical Localization of Heparan Sulfate Proteoglycan in Human Decidual Cell Secretory Bodies and Placental Fibrinoid1

Abstract

A distinct ultrastructural feature of human decidual cells is the presence of membrane-bound secretory bodies, 0.3-0.5 micron in diameter, located within club-shaped processes at the cell periphery. These secretory bodies contain 30-60 nm electron-dense granules. Using specific antibody and the protein A-gold technique, we examined the localization of heparan sulfate proteoglycan in human decidual cells. Morphometric analysis of gold particles in cellular compartments was performed with a Zeiss Videoplan computer system. Immuno-gold staining was present in the decidual cell cytoplasm and the extracellular space, especially in the zone of the external lamina. Gold particles, indicating the locale of heparan sulfate proteoglycan, were concentrated over the electron-dense granular material within decidual secretory bodies contained in club-shaped processes at the cell periphery. Immunolabeling of placental fibrinoid was also observed. This report provides the first identification of a specific molecular constituent of decidual secretory bodies and indicates a role for these structures in secretion of the peri-decidual cell extracellular matrix.

Cell Surface Expression of HIP, a Novel Heparin/Heparan Sulfate-binding Protein, of Human Uterine Epithelial Cells and Cell Lines

“…In contrast, laminin expression was confined to basal lamina in stromal tissue of late proliferative stage uteri. The heparan sulfate proteoglycan, perlecan, also has been reported to be expressed by decidualizing stroma cells (26); however, stromal staining for perlecan was much less intense than that of basal lamina (data not shown). As mentioned above, HIP was not detected in stromal cells through the entire proliferative phase of the cycle.…”

“…By post-ovulatory day 13, HIP is also detected in the predecidual cells of the uterine stroma. The HSPG, perlecan, is expressed by human decidual cells (26).…”

Immunohistochemistry of Two Different Types of Placental Fibrinoid

“…lb, c). Secretion of heparan sul fate proteoglycan by decidual cells into the surrounding fib rinoid has been described by Kisalus and Herr [1988]. This finding raises the question whether extravillous trophoblast is the only source of matrix-type fibrinoid or whether in the fetomaternal junctional zone decidual cells may also con tribute to its secretion and possibly also to its degeneration.…”

“…Numerous immunohisto chemical investigations have shown that, in addition to fibrin and fibrinogen, many other substances can be detected in fibrinoid [Moc, I969a-c;Gille et al, 1974: Burstein et al, 1963, 1973Faulk et al, 1975;Oswald and Gerl. 1975: Kisalus and Herr. 1988: Castellucci et ah, 1991: Nanaev et ah, 1993a.…”

A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas.

“…A decidualização resulta em hiperplasia celular e no aumento no número e na complexidade de organelas citoplasmáticas (Abrahamsohn et al, 2002;Benirschke et al, 2006). Em humanos, as células deciduais apresentam junções celulares (Lawn et al, 1971) e possuem uma característica marcante: a presença de processos citoplasmáticos contendo grânulos densos no seu interior (Kisalus, Herr, 1988;Lawn et al, 1971;Stark, Kaufmann, 1973;Wynn, 1965), os quais contém proteoglicanos de sulfato de heparana que são liberados na MEC ao seu redor (Kisalus, Herr, 1988).…”