Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat spinal cord

Merchenthaler, Istvån; Hynes, Mary; Vígh, S.; Shally, A. V.; Petrusz, Peter

doi:10.1016/0006-8993(83)91001-6

Cited by 120 publications

(40 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of CRH mRNA in the spinal cord and adrenal gland correlates well with the reported presence of CRH immunoreactivity in these tissues (9)(10)(11)(12). Additionally, previous reports have demonstrated CRH-like immunoreactivity in the rat and human anterior pituitary, although the cell types containing CRH immunoreactivity differed in these reports (14,15).…”

Section: Discussionsupporting

confidence: 80%

“…Numerous groups have used immunocytochemical methods to show that CRH peptides are present in widespread regions of the brain as well as some nonbrain tissues (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). In order to determine whether this material is synthesized at these sites or transported to these sites from other regions of synthesis, RNA blot analysis was used to detect the presence of CRH mRNA in a variety of tissues (Figs.…”

Section: Tissue Distribution Of Procrh Mrnamentioning

confidence: 99%

“…The portal blood subsequently flows through the anterior pituitary where CRH can influence the secretion of ACTH. CRH has also been immunocytochemically localized in other regions of the brain and in various peripheral tissues (4)(5)(6)(8)(9)(10)(11)(12)(13)(14)(15) where it may mediate other aspects of the mammalian stress response.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rat Corticotropin-Releasing Hormone Gene: Sequence and Tissue-Specific Expression*

Thompson

Seasholtz

Herbert

1987

Molecular Endocrinology

209

View full text Add to dashboard Cite

The rat corticotropin releasing hormone (CRH) gene has been isolated and characterized by DNA sequence analysis. The gene exhibits a structural organization similar to that of the human CRH gene. The nucleotide sequence encoding the entire rat CRH precursor is located on the second exon, while exon I encodes the 5'-untranslated region of the mRNA. Analysis of the nucleotide sequence homology between the human and rat CRH genes reveals several highly conserved regions including the CRH peptide-encoding sequence and the 5'-flanking sequence. RNA blot analysis demonstrates that CRH mRNA can be observed in numerous regions of the rat brain as well as the spinal cord, adrenal gland, pituitary, and testis.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Tissue Distribution Of Procrh Mrnamentioning

confidence: 99%

See 1 more Smart Citation

Rat Corticotropin-Releasing Hormone Gene: Sequence and Tissue-Specific Expression*

Thompson

Seasholtz

Herbert

1987

Molecular Endocrinology

209

View full text Add to dashboard Cite

show abstract

“…administration. CRF may act on areas with low densities of receptors or, alternatively, it may have actions in the spinal cord where, in the rat, large numbers of CRF immunoreactive fibres and terminals have been found (Merchenthaler et al, 1983). Further studies using more discrete injections are required to localise the sites of action of CRF.…”

Section: Discussionmentioning

confidence: 99%

The effects of naloxone on the cardiovascular and respiratory effects of centrally administered corticotrophin releasing factor in conscious rabbits

May

Whitehead

1991

British J Pharmacology

View full text Add to dashboard Cite

1 The effects of intracerebroventricular (i.c.v.) and intracisternal (i.c.) administration of corticotrophin releasing factor (CRF) (0.5 nmol kg-1) were examined in conscious rabbits. The effect of opioid receptor antagonism was examined to determine whether the responses to CRF were mediated by endogenous opioid peptides. 2 After i.c.v. CRF there was a rise in mean arterial pressure (MAP), heart rate (HR), plasma noradrenaline and adrenaline, and increased behavioural activity. Respiration rate increased, Paco2 fell, but Pao2 was unchanged. 3 The pressor and behavioural effects of i.c.v. CRF were unaltered by high doses of intravenous naloxone (9pmolkg-' bolus followed by 9pumolkg-1min-m infusion); these effects of CRF were also not prevented by double this dose of naloxone. Naloxone attenuated the CRF-induced tachycardia, blocked the increase in respiration rate and increased the fall in Paco2. 4 After i.c. CRF (0.5 nmol kg-) there were similar changes in MAP, HR, plasma catecholamines, respiration and behaviour. 5 These results indicate that in conscious rabbits the pressor effects of i.c.v. CRF are not mediated by endogenous opioid peptides. The finding that the effects of CRF were similar after i.c.v. and i.c. administration suggests that these responses may result from actions on brainstem rather than periventricular sites.

show abstract

“…However, it is now clear that CRF is not confined to the hypothalamus and the stress axis. There are extensive morphological and physiological data that establish the presence of and a role for CRF in cerebellar circuits in many mammalian species including, mouse, rat, cat, rabbit, opossum, sheep, nonhuman primates, and humans (Bloom et al 1982;Olschowka et al 1982;Merchenthaler et al 1983;Merchenthaler 1984;Powers et al 1987;Kitahama et al 1988;Cummings et al 1988;Mugnaini and Nelson 1989;Watabe et al 1991;Potter et al 1994;Yamano and Tohyama 1994;Cummings et al 1994;Bishop and King 1999).…”

Section: Corticotropin-releasing Factor Family Of Peptidesmentioning

confidence: 98%

Modulatory Role of Neuropeptides in the Cerebellum

Bishop¹,

King²

2013

Handbook of the Cerebellum and Cerebellar Disorders

View full text Add to dashboard Cite

The existence of neuropeptides in the central nervous system has been known for almost 40 years. Within the cerebellum approximately 22 neuropeptides have been identified. However, the functional role(s) for many of the different peptides in the cerebellum is largely unknown as their distribution in the cerebellum is not uniform and varies between species. Further, to date sufficient physiological analysis has not been carried out to determine the potential importance of cerebellar peptides. It has been proposed that peptides slowly modulate cerebellar circuits by acting through G protein-coupled receptors in contrast to the fast action of amino acid neurotransmitters that bind to ionotropic receptors. This effect, in turn, would influence the state of an entire neuronal circuit rather than a single neuron. This chapter will be focused on two members of the corticotropin-releasing factor (CRF) family of peptides, namely, corticotropin-releasing factor and urocortin because their distribution has been described in multiple species and their function has been investigated to a greater extent than any of the other peptides found in the cerebellum. A review of the distribution of these peptides as well as a discussion of their cognate receptors is included. Data on the functional role of both peptides is presented. It is clear that complex interactions take place between these peptides and other transmitters in the cerebellum. The physiological data suggest that CRF is essential for normal cerebellar-mediated motor performance. There is less data on the physiological role of urocortin (UCN) in the cerebellum. To date, it has been shown that UCN increases the spontaneous firing rate

show abstract

Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat spinal cord

Cited by 120 publications

References 24 publications

Rat Corticotropin-Releasing Hormone Gene: Sequence and Tissue-Specific Expression*

Rat Corticotropin-Releasing Hormone Gene: Sequence and Tissue-Specific Expression*

The effects of naloxone on the cardiovascular and respiratory effects of centrally administered corticotrophin releasing factor in conscious rabbits

Modulatory Role of Neuropeptides in the Cerebellum

Contact Info

Product

Resources

About