Immunocytochemical detection of dentine matrix protein 1 in experimentally induced reactionary and reparative dentine in rat incisors

Aguiar, Marcio Cajazeira; Arana-Chavez, Victor E.

doi:10.1016/j.archoralbio.2010.01.005

Cited by 15 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, these cells treated with odontogenic medium showed high DMP1 expression, suggesting that this protein plays a role regulating specific genes controlling odontoblast-like differentiation (18). Those authors additionally showed that DMP1 is expressed in the odontoblast-like cells aligned along the reparative dentin-pulp interface.…”

Section: Discussionmentioning

confidence: 94%

In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

et al. 2012

View full text Add to dashboard Cite

The aim of this study was to evaluate the odontogenic potential of undifferentiated pulp cells (OD-21 cell line) through chemical stimuli in vitro. Cells were divided into uninduced cells (OD-21), induced cells (OD-21 cultured in supplemented medium/OD-21+OM) and odontoblast-like cells (MDPC-23 cell line). After 3, 7, 10 and 14 days of culture, it was evaluated: proliferation and cell viability, alkaline phosphatase activity, total protein content, mineralization, immunolocalization of dentin matrix acidic phosphoprotein 1 (DMP1), alkaline phosphatase (ALP) and osteopontin (OPN) and quantification of genes ALP, OSTERIX (Osx), DMP1 and runtrelated transcription factor 2 (RUNX2) through real-time polymerase chain reaction (PCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests (p<0.05). There was a decrease in cell proliferation in OD-21 + OM, whereas cell viability was similar in all groups, except at 7 days. The amount of total protein was higher in group OD-21 + OM in all periods; the same occurred with ALP activity after 10 days when compared with OD-21, with no significant differences from the MDPC-23 group. Mineralization was higher in OD-21+OM when compared with the negative control. Immunolocalization demonstrated that DMP1 and ALP were highly expressed in MDPC-23 cells and OD-21 + OM cells, whereas OPN was high in all groups. Real-time PCR revealed that DMP1 and ALP expression was higher in MDPC-23 cell cultures, whereas RUNX2 was lower for these cells and higher for OD-21 negative control. Osx expression was lower for OD-21 + OM. These results suggest that OD-21 undifferentiated pulp cells have odontogenic potential and could be used in dental tissue engineering.

show abstract

Section: Discussionmentioning

confidence: 94%

In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Our previous publication showed that RGD peptide improved mineralized tissue formation on silk based scaffolds 20. To expand our earlier studies, here we also examined the utility of DMP‐1 containing silk scaffolds, based on the fact that this acidic and phosphorylated extracellular matrix protein is expressed in odontoblast, predentin, and dentin,30 and that loss of DMP‐1 results in dentin anomalies, including partial failure of predentin maturation into dentin, and hypomineralization 31. Enhanced osteo‐integration was also observed in DMP‐1 pretreated implants 32.…”

Section: Discussionmentioning

confidence: 99%

Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds

Zhang

Ahluwalia

Literman

et al. 2011

J Biomedical Materials Res

View full text Add to dashboard Cite

Silk scaffolds have been successfully used for a variety of tissue engineering applications due to their biocompatibility, diverse physical characteristics, and ability to support cell attachment and proliferation. Our prior characterization of 4-day postnatal rat tooth bud cells grown on hexafluoro-2-propanol (HFIP) silk scaffolds showed that the silk scaffolds not only supported osteodentin formation, but also guided the size and shape of the formed osteodentin. In this study, interactions between human dental pulp cells and HFIP and aqueous based silk scaffolds were studied under both in vitro and in vivo conditions. Silk scaffold porosity and incorporation of RGD and DMP peptides were examined. We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or without additional cells. We conclude that alternative silk scaffold materials, and hDSC pre-seeding cell treatments or sorting and enrichment methods, need to be considered for successful dental hard tissue regeneration.

show abstract

“…DSPP is the major dentinal noncollagenous protein and plays a crucial role during dentin mineralization (28). DMP-1 is another specific protein for dentin and is mostly known as the candidate gene for the disorder dentinogenesis imperfecta (29). These dentin-related morphogens are important for the differentiation of DPSC into odontoblasts and for the engineering of dental pulp-like tissues (30).…”

Section: R E T R a C T E Dmentioning

confidence: 99%

RETRACTED: Mineralized Tissue Formation by Bone Morphogenetic Protein-7–transfected Pulp Stem Cells

Yang

Zhang

Pang

et al. 2012

Journal of Endodontics

View full text Add to dashboard Cite

Immunocytochemical detection of dentine matrix protein 1 in experimentally induced reactionary and reparative dentine in rat incisors

Cited by 15 publications

References 20 publications

In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

In vitro evaluation of the odontogenic potential of mouse undifferentiated pulp cells

Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds

RETRACTED: Mineralized Tissue Formation by Bone Morphogenetic Protein-7–transfected Pulp Stem Cells

Contact Info

Product

Resources

About