Immunocompetence and duration of immunity againstVibrio salmonicidaandAeromonas salmonicidaafter vaccination of Atlantic salmon (Salmo salarL.) at low and high temperatures

LitR of Vibrio salmonicida Is a Salinity-Sensitive Quorum-Sensing Regulator of Phenotypes Involved in Host Interactions and Virulence

“…For challenge experiments, fish were exposed to V. salmonicida cells that were recently passaged through fish to verify and prepare virulence (16,61). The challenge doses were established based on a prechallenge experiment as well as on previous studies (7,12,47,48). Mortality was monitored daily for a period of 3 weeks.…”

“…Current commercial vaccines give full protection against infection with V. salmonicida (12), and the bacterium is no longer an immediate threat to the salmonid aquaculture industry. However, the molecular mechanisms of host specificity, invasion, colonization and virulence are still largely unknown, making V. salmonicida an interesting bacterial species for studying host-pathogen interaction and pathogenesis.…”

Detection of the herpesviral hematopoietic necrosis disease agent (Cyprinid herpesvirus 2) in moribund and healthy goldfish: validation of a quantitative PCR diagnostic method

“…Another approach is to look for antiviral antibodies that would indicate prior virus exposure, but in fish the duration of detectable titers against specific pathogens is quite variable (Vestergaard & Jørgensen 1982, Eggset et al 1997, Bricknell et al 1999). More problematic is that antibodies may not be present in fish that carry the virus but that have not yet developed the acute disease.…”

Antibodies against Vibrio salmonicida lipopolysaccharide (LPS) and whole bacteria in sera from Atlantic salmon (Salmo salar L.) vaccinated during the smolting and early post-smolt period

“…The vaccination of Atlantic salmon with whole bacteria of V. salmonicida has been proved to be successful by use of di#erent vaccines with and without adjuvants (Eggset et al, 1997b;Hjeltnes et al, 1989;Lillehaug, 1989Lillehaug, , 1990Lillehaug et al, 1990Lillehaug et al, , 1993. For practical and fish health reasons, vaccination of salmon in Norway is performed at water temperatures as low as 1-3 C and a study by Lillehaug et al (1993) has shown that the antibody levels to V. salmonicida were highest for salmon vaccinated at low temperatures.…”