Immunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis

Portnyagina, O. Yu.; Sidorova, O. V.; Khomenko, V. A.; Novikova, O. D.; Issaeva, Marina; Соловьева, Т. Ф.

doi:10.5772/35207

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Increase of myeloperoxidase level indirectly characterizes oxygen-dependent killing of bacteria in phagocyte (complete phagocytosis), therefore it shows that the three-time immunization of mice with RP (100 µg/mouse) leads not only to the synthesis of specific antibodies but stimulates the enhancement of induced bactericidal activity of peritoneal macrophages as well. This result coincides with previous data that increased activity of macrophages is a result of experimental pseudotuberculosis infection (Timchenko et al, 1990) and immunization with the porin isolated (Portnyagina et al, 1999).…”

Section: Stimulation Of Murine Macrophages By Recombinant Porinsupporting

confidence: 93%

“…5). Quite possible, the linear antigen determinants (probably formed at the level of primary and/or secondary porin structure) served as common parts of RP and native porin (Portnyagina et al, 1999). At the same time discontinuous (or conformational) antigen determinants (forming by closing in some parts of protein amino acid sequence during assembling 3D protein structure) were absent or were not properly formed.…”

Section: Antigenic Structure Of Recombinant Porinsmentioning

confidence: 99%

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Trends in Immunolabelled and Related Techniques

Abuelzein¹

2012

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with the B.V.Sc. in Veterinary Medicine. He obtained his Master's and Ph.D. degrees in virology from the University of Reading, Britain. He also got a Post-Doctoral Fellowship (FIAEA), in Immunolabelled Techniques & Arbovirology , the World Reference Laboratory, Pirbright Britain. Since 1975 he has been engaged in active research, technological developments and teaching in several Universities in Sudan and Saudi Arabia. He acted as a Consultant to the International Atomic Energy Agency (IAEA) in Immunolabelled Techniques. He is an Editor and reviewer for a number of Scientific Journals including the International Journal "Tropical Animal Health & Production", Britain. Published 123 peer reviewed papers in reputable scientific journals and 60 peer reviewed articles in conferences. He was the first to develop the ELISA technique in Foot and mouth disease (FMD) Virus Research. Quoted in the highly reputed international scientific journal "Nature". Contributed in the Book: "Immunoassay in the 80's", 1981, ATP publishing Co., London. Made first records of several viral diseases of great international significance.

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Section: Stimulation Of Murine Macrophages By Recombinant Porinsupporting

confidence: 93%

Section: Antigenic Structure Of Recombinant Porinsmentioning

confidence: 99%

Trends in Immunolabelled and Related Techniques

Abuelzein¹

2012

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Molecular Characterization, Phylogenetic, Expression, and Protective Immunity Analysis of OmpF, a Promising Candidate Immunogen Against Yersinia ruckeri Infection in Channel Catfish

Wang

Qin

et al. 2018

Front. Immunol.

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Outer membrane porins, as the major components of Gram-negative bacterial membrane proteins, have been proven to be involved in interactions with the host immune system and potent protective antigen candidates against bacterial infection in fish. Outer membrane porin F (OmpF) is one of the major porins of Yersinia ruckeri (Y. ruckeri), the causative agent of enteric red mouth disease of salmonid and non-salmonid fish. In the present study, the molecular characterization and phylogenetic analysis of OmpF gene was studied, heterogenous expression, immunogenicity and protective immunity of OmpF were systemically evaluated as a subunit vaccine for channel catfish against Y. ruckeri infection. The results showed that OmpF gene was highly conserved among 15 known Yersinia species based on the analysis of conserved motifs, sequences alignment and phylogenetic tree, and was subjected to negative/purifying selection with global dN/dS ratios value of 0.649 throughout the evolution. Besides, OmpF was also identified to have immunogenicity by western blotting and was verified to be located on the surface of Y. ruckeri using cell surface staining and indirect immunofluorescence assays. Moreover, recombinant OmpF (rtOmpF) as a subunit vaccine was injected with commercial adjuvant ISA763, significantly enhanced the immune response by increasing serum antibody levels, lysozyme activity, complement C3 activity, total protein content, SOD activity, immune-related genes expression in the head kidney and spleen, and survival percent of channel catfish against Y. ruckeri infection. Thus, our present results not only enriched the information of molecular characterization and phylogenetics of OmpF, but also demonstrated that OmpF holds promise to be used as a potential antigen against Y. ruckeri infection in fish.

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Immunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis

Cited by 2 publications

References 21 publications

Trends in Immunolabelled and Related Techniques

Trends in Immunolabelled and Related Techniques

Molecular Characterization, Phylogenetic, Expression, and Protective Immunity Analysis of OmpF, a Promising Candidate Immunogen Against Yersinia ruckeri Infection in Channel Catfish

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