Immunochemical Characterization of Type i Carbohydrate Antigen of “Streptococcus milleri” (Streptococcus anginosus)

Oral Microbiology and Immunology

1996

Carbohydrate antigens of the serotype k/Lancefield group G "Streptococcus milleri" were extracted by autoclaving whole cells of the type k reference strain Streptococcus anginosus K214-2K. The type k and group G antigen molecules are separated from each other and partially purified by a DEAE-Sephadex A25 column chromatography followed by a Sephadex G-100 gel filtration. In the double diffusion and the immunoelectrophoresis, the type k and group G antigen preparations obtained yielded single bands with their homologous antisera respectively. The type k antigen preparation contained principally glycerol, rhamnose, ribitol, galactose and glucose in a molar ratio of 0.54:1.20:0.43:0.33:1.00. The quantitative precipitin inhibition test indicated that beta(1-6)glucosyl-glucose (gentiobiose) sequence played a major role in immunodeterminant structure. Thus, the type k antigen of "S. milleri" appears to be a new carbohydrate type antigen that is chemically different from any Ottens type antigen. In contrast, the group G antigen preparation contained high proportion of rhamnose in addition to glucose, N-acetylglucosamine and N-acetylgalactosamine in a molar ratio of 6.45:1.00:0.24:1.15, and the rhamnose residue was involved in the immunodominant epitope, being in good agreement with the previously proposed chemical structure of the group antigen.

mentioning

confidence: 99%

Immunochemical characterization of the carbohydrate antigens of serotype k and Lancefield group G “Streptococcus milleri”

Kitada

Oral Microbiology and Immunology

1996

“…As previously demonstrated, "S. milleri" strains isolated frotn the hutnan mouth show wide serological variations in their cell surface carbohydrate antigens (18,20), The recent itnmunochemical studies have revealed that serotype b-speciftc carbohydrate antigen is composed of rhamnose and glucose and its itntnunodetertninant teniiinal is a rhamnose residue (21), Additionally, serotype i-specific antigen is a polysaceharide consisting of galactose as an imtnunodotiiinant cotnpotient in combination with rhatnnose and glucose (7). Both oi these type antigens are different from the Lancefield group antigens A to G (20), In this study, serotype g-specific antigens of "S, milleri" were extracted with TCA, purified using column chromatography and characterized itnmunochetnically.…”

Section: Discussionmentioning

confidence: 91%

“…Neutral sugars and amino sugars were quantitatively analyzed using gas-liquid chrotnatography as described previously (7,21), Antigen preparations (1-5 tng) were hydrolyzed in 2 tnl of 3 NHClat 100°Cfor4h, Total hexoses and N-acetyl-hexosatnines were estitnated by the anthron method (14) and by the method described by Ghuysen et al, (4), respectively. Phosphorus and protein were quantified by the colorimetric tnethods of Lowry et al, (8) and Lowry et al, (9), respectively.…”

Section: Chemical and Immunoiogicai Methodsmentioning

confidence: 99%

“…It is known that strains of "5, milteri" often carry Lancefield group antigens and/or Ottens-type antigens (6), but many isolates from various clinical specimens have been reported to be serologically ungroupable and untypeable(l, 6), Recently, we have isolated a large number of "S. milleri" strains from human dental plaque (18,19), These isolates detnonstrated wide serological Variations based on their cell surface carbohydrate antigens (19,20), The type-speciftc antigens of serotype b and i "S. milleri" have been characterized immunochetnicaJiy in our previous study (7,21), This article describes the ehetnical cotnposition and imtnunological properties of the typespecific carbohydrate antigen purified from the type strain of serotype g "S. milleri". iVIateriai and metiiods "S. miileri" strains "5", milleri" strain KIK (serotype g reference strain (20)) was used.…”

mentioning

confidence: 99%

“…iVIateriai and metiiods "S. miileri" strains "5", milleri" strain KIK (serotype g reference strain (20)) was used. Cells were grown anaerobically at 37 °C for 18 h in 1 % glucose broth, then washed with distilled water and lyophilized as described previously (7), Preparation and purification of ceil wails and type antigen Serotype g-specific carbohydrate antigen was purified frotn the cell walls of strain KIK, The entire description of the procedures has been reported previously (7,21), Very briefiy, whole cells were tnechanically disrupted in a cell hotnogenizer (tnodel MSK; B, Braun Apparatebau, Melsungen, FRG), The cell walls were collected by centrifugation and then treated with trypsin (0,4 tng/tnl; 1:250, Difco Laboratories, Detroit, MI) at pH 7,0 at 37°C for 2 b. The digested cell wails were washed in distilled water and lyophilized.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Carbohydrate antigen of serotype g “Streptococcus milleri”: immunochemical characterization

Oral Microbiology and Immunology

Yakushiji

Konagawa

1991

Self Cite

Cell wall carbohydrate antigen of the serotype g "Streptococcus milleri" was extracted with cold trichloroacetic acid from purified cell walls of the type strain K1K. The extracts were then purified by a DEAE-Sephadex A-25 column, followed by a Sephadex G-100 column. The immunoelectrophoresis revealed that the serotype g carbohydrate antigen preparation displayed a single precipitin band against the crude anti-K1K serum. The purified type g antigen consisted of rhamnose, galactose, glucose and galactosamine in a ratio of 1.3:3.8:1.0:2.5. The quantitative precipitin inhibition test with various haptens indicated that galactosamine is a major immunodeterminant of the type g-specific antigen.

Serotype variation in Streptococcus anginosus, S. constellatus and S. intermedius

Journal of Medical Microbiology

Eifuku-Koreeda²,

Kitada³

et al. 1998

Various serotypes reported in three serotyping systems for the 'Streptococcus milleri' group, i.e., Ottens I-IV, Osano I-IV and serotypes a-k, were compared with each other in a capillary precipitation test and by the double immunodiffusion test. Only two of the 19 serotypes, f and Ottens-111, were identical, and the other 17 serotypes were independent. Thus, 18 serotypes and another three candidates (a-, b-and g-crossreactive) were found in the 'S. milleri' group. Of the 248 strains tested, 197 were serotypable, and 23 strains carried two type antigens. Generally, the Ottens I, 11, 111 (serotype j ) , IV and Osano IV antigens, often together with the Lancefield group F antigen, were found in S. anginosus and S. constellatus. In addition, the serotype a, c, d, e and k antigens with the group A, C or G antigen were distributed in S. anginosus, the serotype b antigen in S. constellatus and the g, h, i, j , Osano I, I1 and I11 antigens in S. intermedius. Twenty-five of the untypable strains were Lancefield groupable (mostly F).