Immunoblot with Cerebrospinal Fluid from Patients with Neurocysticercosis using Antigen from Cysticerci of Taenia Solium and Taenia Crassiceps

Vaz, Adelaide José; Nunes, Cáris Maroni; Piazza, Roxane M. F.; Livramento, José Antônio; Silva, M. V. Da; Nakamura, Paulo Mutuko; Ferreira, Antônio Walter

doi:10.4269/ajtmh.1997.57.354

Cited by 95 publications

(99 citation statements)

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“…The ELISA reaction was processed in accordance with the technique of Costa et al 19 . Immunoblotting was performed as per Vaz et al 20 . IFT and immunoblotting were used only as qualitative reactions.…”

Section: Methodsmentioning

confidence: 99%

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

Machado

Livramento

Vaz

et al. 2002

Arq. Neuro-Psiquiatr.

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-We analyzed cerebrospinal fluid (CSF) and blood serum from 55 patients with neurocysticercosis (NC) at different clinical stages. According to inflammatory activity in the CSF, three stages were identified: (1) reactive, when there was at least an increase in the number of cells; (2) weakly reactive, when significant alterations were found in the CSF, including an increase in gamma globulins, albeit without hypercytosis; (3) non-reactive, when there was neither hypercytosis nor increase in gamma globulins. Nineteen patients had the reactive form; 18 had the weakly reactive form; 18 displayed the non-reactive form.

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Section: Methodsmentioning

confidence: 99%

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

Machado

Livramento

Vaz

et al. 2002

Arq. Neuro-Psiquiatr.

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“…One of the difficulties in such studies is obtaining parasites from the model of natural infection in swine. Taenia crassiceps metacestodes, found in rodents, have been described in natural and experimental infections, and have been used as an alternative source of antigen in immunological tests for NC 2,[7][8][9][10][11] .…”

mentioning

confidence: 99%

Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

Bueno¹,

Vaz

Machado

et al. 2000

Arq. Neuro-Psiquiatr.

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-We assayed samples of cerebrospinal fluid (CSF), serum and saliva from patients with neurocysticercoses, control group and individuals with other parasitoses, by ELISA with Taenia crassiceps vesicular fluid antigen (Tcra) and Taenia solium total antigen (Tso) for the detection of antibodies. The sensitivity for IgG-Tcra was 100% for CSF and serum, and 32.0% for saliva; and for IgG-Tso 100% for CSF, 80.0% for serum and 24.% for saliva. Specificity was 100% for CSF and 80.0% for serum with both antigens, and 100% for saliva with Tcra and 87.5% with Tso. The sensitivity and specificity for IgA-Tcra was, respectively, 40.0% and 100% for CSF, 36.0% and 97.1% for serum, and 4.0% and 90.0% for saliva. IgE detection showed 24.0% sensitivity and 97.1% specificity for serum, with no detection in CSF samples. The search for antibodies revealed the presence of IgG > IgA > IgE in CSF, serum and saliva samples, with IgG being present in all phases of the disease, while IgA/IgE were more frequent in the inactive form.KEY WORDS: cysticercosis, central nervous system, humoral immune response, IgG, IgE, IgA, T. crassiceps.Neurocisticercose: detecção de anticorpos IgG, IgA e IgE em amostras de líquido cefalorraquidiano, soro e saliva por ELISA com antígenos de Taenia solium e Taenia crassiceps RESUMO -Analisamos amostras de líquido cefalorraquidiano (LCR), soro e saliva de pacientes com neurocisticercose, grupo de controle e indivíduos com outras parasitoses, por ELISA com antígenos de líquido vesicular de T. crassiceps (Tcra) e salino total de T. solium (Tso) para a pesquisa de anticorpos. A sensibilidade para IgG-Tcra foi 100% para LCR e soro e 32,0% para saliva, e para IgG-Tso 100% para LCR, 80,0% para soro e 24,0% para saliva. A especificidade foi de 100% para CSF e 80,0% para soro com ambos os antígenos, e 100% para saliva com Tcra e 87,5% com Tso. A sensibilidade e especificidade para IgA-Tcra foi, respectivamente: 40,0% e 100% para LCR, 36,0% e 97,1% para soro, e 4,0% e 90,0% para saliva. A pesquisa de IgE mostrou 24,0% de sensibilidade e 97,1% de especificidade para soro, sem detecção nas amostras de LCR. A pesquisa de anticorpos revelou a presença de IgG > IgA > IgE no LCR, soro e saliva, com presença de IgG em todas as fases evolutivas da doença, enquanto que IgA/IgE foram mais frequentes na forma inativa.

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“…The feasibility of this congeneric species approach has already been demonstrated by the diagnoses of patients who are infected with parasites that share epitopes, such as S. stercoralis and Strongyloides venezuelensis (Feliciano et al 2010) or Strongyloides ratti (Rodrigues et al 2007). This approach has been particularly promising in the development of alternative methods for the diagnoses of cisticercosis, strongyloidiasis and hidatidosis (Vaz et al 1997, Sako et al 2006, Feliciano et al 2010, Oliveira et al 2010, da S Ribeiro et al 2010). In addition, Dekumyoy et al (2000) studied the use of a crude antigen preparation that was derived from A. costaricensis as a heterologous target for the detection of antibodies in patients with eosinophilic meningitis.…”

Section: Discussionmentioning

confidence: 99%

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Ben

Rodrigues

Agostini

et al. 2010

Mem. Inst. Oswaldo Cruz

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Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in intestinal tissues due to intense eosinophilic inflammation. Because A. costaricensis is difficult to maintain in the laboratory, an immunodiagnostic IgG enzyme-linked immunosorbent assay (ELISA) using antigens from the congeneric Angiostrongylus cantonensis species was evaluated against a panel of serum samples from patients who were histologically diagnosed with A. costaricensis infections. Sera from uninfected individuals and individuals infected with other parasites were used as controls. The sensitivity and specificity of the assay were estimated at 88.4% and 78.7%, respectively. Because the use of purified or cloned antigens has not been established as a reliable diagnostic tool, the use of heterologous antigens may provide a viable alternative for the development of an ELISA-based immunodetection system for the diagnosis of abdominal angiostrongyliasis

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Immunoblot with Cerebrospinal Fluid from Patients with Neurocysticercosis using Antigen from Cysticerci of Taenia Solium and Taenia Crassiceps

Cited by 95 publications

References 0 publications

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

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