Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells

Hosoya, Shoichi; Villena, Julio; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hiromi; Chiba, Eriko; Shimosato, Takeshi; Aso, Hisashi; Suda, Yoshihito; Kawai, Yasushi; Saito, Tadao; Álvarez, Susana; Ikegami, Shuji; Itoh, Hiroyuki; Kitazawa, Haruki

doi:10.1186/1297-9716-42-111

Cited by 45 publications

(61 citation statements)

References 34 publications

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“…The binding of dsRNA ligand poly(I·C) to TLR3, RIG-I, or MDA5 has been reported to activate the downstream signaling cascade that ends with the induction of type I interferons (IFNs) and the production of cytokines/chemokines (a T H 1 response) (10,11,14). The results of the poly(I·C)-only challenge in HT-29 cells of our study are in accordance with previously published data on the upregulation of a number of proinflammatory and antiviral genes connected to the TLR3-TRIF, NF-B, and MAPK signaling pathways (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Localized in the endosomal membrane of IECs, TLR3 triggers the activation of the innate antiviral immune response against dsRNA viruses (10,11). However, new evidence has emerged showing that two cytoplasmic PRRs of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, RIG-I and melanoma differentiation-associated gene 5 (MDA5), are major sensors for dsRNA viruses, such as rotavirus, and for cell signaling of type I IFN (12,13).…”

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confidence: 99%

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Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

MacPherson

Audy

Mathieu

et al. 2014

Appl Environ Microbiol

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A commercially available product containing three probiotic bacterial strains (Lactobacillus helveticus R0052, Bifidobacterium longum subsp. infantis R0033, and Bifidobacterium bifidum R0071) was previously shown in animal trials to modulate both T H 1 and T H 2 immune responses. Clinical studies on this combination of bacteria have also shown positive health effects against seasonal winter diseases and rotavirus infection. The goal of this study was to use a well-established in vitro intestinal epithelial (HT-29) cell model that has been shown to constitutively express double-stranded RNA (dsRNA) sensors (Toll-like receptor 3 [TLR3], retinoic acid-inducible gene I, melanoma differentiation-associated gene 5, and dsRNA-activated protein kinase). By using the HT-29 cell model, we wanted to evaluate whether or not this combination of three bacteria had the capacity to immune modulate the host cell response to a dsRNA ligand, poly(I·C). Using a custom-designed, two-color expression microarray targeting genes of the human immune system, we investigated the response of HT-29 cells challenged with poly(I·C) both in the presence and in the absence of the three probiotic bacteria. We observed that the combination of the three bacteria had a major impact on attenuating the expression of genes connected to proinflammatory T H 1 and antiviral innate immune responses compared to that obtained by the poly(I·C)-only challenge. Major pathways through which the multistrain combination may be eliciting its immune-modulatory effect include the TLR3 domain-containing adapter-inducing beta interferon (TRIF), mitogenactivated protein kinase, and NF-B signaling pathways. Such a model may be useful for selecting potential biomarkers for the design of future clinical trials.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

MacPherson

Audy

Mathieu

et al. 2014

Appl Environ Microbiol

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“…Suspensions of porcine PP immunocompetent cells were prepared from the ilea of adult swine as previously described (16,31). All procedures were conducted in accordance with the Guidelines for Animal Experimentation of Tohoku University, Sendai, Japan.…”

Section: Methodsmentioning

confidence: 99%

Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells

Villena

Suzuki

Fujie

et al. 2012

Clin. Vaccine Immunol.

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121

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“…These cells are permissive to porcine RVs and also respond to dsRNA and its synthetic analog poly(I:C) (49, 50). Furthermore, co-cultures of PIE cells with immune cells isolated from porcine Peyer’s patches (PPs) provide an in vitro system to study the transduction of the signal from its detection by IECs to the effect on the under laying immune cells.…”

Section: Cellular and Molecular Mechanisms Of Immunobiotics Actionsmentioning

confidence: 99%

Intestinal Innate Antiviral Immunity and Immunobiotics: Beneficial Effects against Rotavirus Infection

2016

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The mucosal tissues of the gastrointestinal tract are the main portal entry of pathogens such as rotavirus (RV), which is a leading cause of death due to diarrhea among young children across the globe and a major cause of severe acute intestinal infection in livestock animals. The interactions between intestinal epithelial cells (IECs) and immune cells with RVs have been studied for several years, and now, it is known that the innate immune responses triggered by this virus can have both beneficial and detrimental effects for the host. It was demonstrated that natural RV infection in infants and experimental challenges in mice result in the intestinal activation of pattern recognition receptors (PRRs) such as toll-like receptor 3 (TLR3) and striking secretion of proinflammatory mediators that can lead to increased local tissue damage and immunopathology. Therefore, modulating desregulated intestinal immune responses triggered by PRRs activation are a significant promise for reducing the burden of RV diseases. The ability of immunoregulatory probiotic microorganisms (immunobiotics) to protect against intestinal infections, such as those caused by RVs, is among the oldest effects studied for these important group of beneficial microbes. In this review, we provide an update of the current status on the modulation of intestinal antiviral innate immunity by immunobiotics and their beneficial impact on RV infection. In addition, we describe the research of our group that demonstrated the capacity of immunobiotic strains to beneficially modulated TLR3-triggered immune response in IECs, reduce the disruption of intestinal homeostasis caused by intraepithelial lymphocytes, and improve the resistance to RV infections.

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Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells

Cited by 45 publications

References 34 publications

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

Multistrain Probiotic Modulation of Intestinal Epithelial Cells' Immune Response to a Double-Stranded RNA Ligand, Poly(I·C)

Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells

Intestinal Innate Antiviral Immunity and Immunobiotics: Beneficial Effects against Rotavirus Infection

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