Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells

Immunoregulatory Effects Triggered by Lactic Acid Bacteria Exopolysaccharides: New Insights into Molecular Interactions with Host Cells

“…Those studies clearly showed a key role for TLR2 in the anti-inflammatory effect of immunobiotic bacteria. In agreement, our studies in PIE cells and porcine immune cells also revealed that TLR2 plays an important role in the immunoregulatory activity of immunobiotic bacteria [ 34 , 38 , 76 , 77 ]. Our studies of the anti-inflammatory activity of L. jensenii TL2937, B. breve MCC-117, and B. longum BB536 in PIE cells showed that TLR2 is necessary for the immunoregulatory capacities of those bacteria.…”

Immunoregulatory Effects Triggered by Lactic Acid Bacteria Exopolysaccharides: New Insights into Molecular Interactions with Host Cells

“…Those studies clearly showed a key role for TLR2 in the anti-inflammatory effect of immunobiotic bacteria. In agreement, our studies in PIE cells and porcine immune cells also revealed that TLR2 plays an important role in the immunoregulatory activity of immunobiotic bacteria [ 34 , 38 , 76 , 77 ]. Our studies of the anti-inflammatory activity of L. jensenii TL2937, B. breve MCC-117, and B. longum BB536 in PIE cells showed that TLR2 is necessary for the immunoregulatory capacities of those bacteria.…”

Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937

“…Most notable changes were found in chemokines, since expression levels of CCL2, CCL8, CXCL5, CXCL9, CXCL10, and CXCL11 were significantly lower in TL2937-treated PIE cells when compared to the controls (Figure 7). This finding is of interest because it not only confirms our previous in vitro Tsukida et al, 2016;Villena et al, 2012) and in vivo (Suda et al, 2014) studies demonstrating the anti-inflammatory capacity of L. jensenii TL2937, but also provides a potential set of biomarkers for the screening and selection of new immunoregulatory bacterial strains.…”

Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs

“…In fact, the conditioning of monocyte-derived DCs with IEC supernatants confers on DCs the capacity to produce large amounts of IL-10, which is attributable, at least in part, to the release of IEC-derived factors such as TGF-β [ 56 ]. In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ].…”

“…In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ]. Because the feeding of pigs with L. jensenii TL2937 reduced the recovery of pathogenic E. coli and the levels of inflammation markers, it could be speculated that the immunobiotic TUA4408L-fermented okara feed would be also capable of modulating porcine intestinal antigen-presenting cells.…”

Immunoregulatory Effects Triggered by Lactic Acid Bacteria Exopolysaccharides: New Insights into Molecular Interactions with Host Cells

“…Those studies clearly showed a key role for TLR2 in the anti-inflammatory effect of immunobiotic bacteria. In agreement, our studies in PIE cells and porcine immune cells also revealed that TLR2 plays an important role in the immunoregulatory activity of immunobiotic bacteria [ 34 , 38 , 76 , 77 ]. Our studies of the anti-inflammatory activity of L. jensenii TL2937, B. breve MCC-117, and B. longum BB536 in PIE cells showed that TLR2 is necessary for the immunoregulatory capacities of those bacteria.…”

Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937

“…Most notable changes were found in chemokines, since expression levels of CCL2, CCL8, CXCL5, CXCL9, CXCL10, and CXCL11 were significantly lower in TL2937-treated PIE cells when compared to the controls (Figure 7). This finding is of interest because it not only confirms our previous in vitro Tsukida et al, 2016;Villena et al, 2012) and in vivo (Suda et al, 2014) studies demonstrating the anti-inflammatory capacity of L. jensenii TL2937, but also provides a potential set of biomarkers for the screening and selection of new immunoregulatory bacterial strains.…”

Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs

“…In fact, the conditioning of monocyte-derived DCs with IEC supernatants confers on DCs the capacity to produce large amounts of IL-10, which is attributable, at least in part, to the release of IEC-derived factors such as TGF-β [ 56 ]. In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ].…”

“…In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ]. Because the feeding of pigs with L. jensenii TL2937 reduced the recovery of pathogenic E. coli and the levels of inflammation markers, it could be speculated that the immunobiotic TUA4408L-fermented okara feed would be also capable of modulating porcine intestinal antigen-presenting cells.…”

Immunoregulatory Effects Triggered by Lactic Acid Bacteria Exopolysaccharides: New Insights into Molecular Interactions with Host Cells

“…Those studies clearly showed a key role for TLR2 in the anti-inflammatory effect of immunobiotic bacteria. In agreement, our studies in PIE cells and porcine immune cells also revealed that TLR2 plays an important role in the immunoregulatory activity of immunobiotic bacteria [ 34 , 38 , 76 , 77 ]. Our studies of the anti-inflammatory activity of L. jensenii TL2937, B. breve MCC-117, and B. longum BB536 in PIE cells showed that TLR2 is necessary for the immunoregulatory capacities of those bacteria.…”

Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937

“…Most notable changes were found in chemokines, since expression levels of CCL2, CCL8, CXCL5, CXCL9, CXCL10, and CXCL11 were significantly lower in TL2937-treated PIE cells when compared to the controls (Figure 7). This finding is of interest because it not only confirms our previous in vitro Tsukida et al, 2016;Villena et al, 2012) and in vivo (Suda et al, 2014) studies demonstrating the anti-inflammatory capacity of L. jensenii TL2937, but also provides a potential set of biomarkers for the screening and selection of new immunoregulatory bacterial strains.…”

Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs

“…In fact, the conditioning of monocyte-derived DCs with IEC supernatants confers on DCs the capacity to produce large amounts of IL-10, which is attributable, at least in part, to the release of IEC-derived factors such as TGF-β [ 56 ]. In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ].…”

“…In line with these findings, we demonstrated previously that the immunobiotic strain L. jensenii TL2937 was able to significantly up-regulate TGF-β expression in porcine IECs and, through this mechanism, to improve the synthesis of IL-10 in CD172a + CD11R1 high and CD172a + CD11R1 − porcine intestinal antigen-presenting cells [ 19 , 50 ]. Simultaneously, the direct interaction of the TL2937 strain with CD172a − CD11R1 low cells increased their production of IFN-γ [ 19 , 50 ]. Because the feeding of pigs with L. jensenii TL2937 reduced the recovery of pathogenic E. coli and the levels of inflammation markers, it could be speculated that the immunobiotic TUA4408L-fermented okara feed would be also capable of modulating porcine intestinal antigen-presenting cells.…”