Immunobiological activities of chemically synthesized monosaccharide analogs of lipid A

Shimizu, Tadayori; Masuzawa, Toshiyuki; Yanagihara, Yasutake; Itoh, Hajime; Nakamoto, Shinichi; Shimizu, Chikako; Ikeda, Kiyoshi; Achiwa, Kazuo

doi:10.1016/0192-0561(88)90474-2

Cited by 42 publications

(57 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is not clear whether benzamide induces differentiation in insulin-primed cells by a similar mechanism as the combination dexamethasone/isobutylmethyl xanthine. Induction of differentiation by the latter protocol can be inhibited by 12-0-tetradecanoyl phorbol 13-acetate [50]. The same effect was seen in benzamide-induced differentiation (Table 2) where the phorbol ester completely prevented the appearance of glycerol-3-phosphate dehydrogenase activity.…”

Section: Modification Of Hmg Protcinssupporting

confidence: 52%

Differentiation of 3T3‐L1 pre‐adipocytes induced by inhibitors of poly(ADP‐ribose) polymerase and by related noninhibitory acids

Janßen

Hilz

1989

European Journal of Biochemistry

View full text Add to dashboard Cite

To analyze a possible involvement of ADP-ribosylation reactions in 3T3-LI pre-adipocyte differentiation, ADP-ribosyltransferase activities is permeabilized cells as well as endogenous amounts of protein-bound monoand poly(ADP-ribose) residues were determined. Also, in vivo labeling with [3H]adenosine of ADP-ribose residues linked to high-mobility-group (HMG) proteins was performed. As an additional probe, the effects of ADPribosylation inhibitors and non-inhibitory analogs were studied. Basal and total poly(ADP-ribose) polymerase activities markedly increased prior to the appearance of the differentiation marker glycerol-3-phosphate dehydrogenase. Despite these apparent changes in activity, however, neither protein-bound poly(ADP-ribose) residue nor mono(ADP-ribosyl) groups in histones, nor the NAD content, changed significantly under these conditions. Furthermore, although HMG protein-associated [3H]ADP-ribose was reduced in differentiating [3H]adenosinelabeled cells, the data suggest altered precursor pool labeling rather than a specific decrease in ADP-ribosylated HMG proteins. Non-participation of ADP-ribosylation reactions in 3T3-LI differentiation is further supported by experiments with inhibitors and non-inhibitory analogs. Benzamide at 0.3 -3 mM per SP without effect on differentiation, was able to induce specific gene expression when combined with insulin (10-l 2 -M). Similar effects were seen with benzoate as well as with nicotinamide, 3-aminobenzamide and their corresponding acids. The data indicate that benzaiiiide and analogs have profound effects on chromatin functions that are not mediated by ADP-ribosylation reactions Eukaryotic cells contain an enzyme, poly(ADP-ribose) polymerase, which uses NAD to modify nuclear proteins by homopolymeric ADP-ribose. In addition, modification of acceptor polypeptides by single ADP-ribosyl groups [mono-(ADP-ribosyl)ation] has been found. Nuclear poly(ADPribosy1)ation appears to be linked to processes like DNA excision repair and carcinogenesis (for reviews see [l -31). The reaction has also been implicated in various differentiation processes [3,4], although opposite changes in different systems have been reported. Thus, in some differentiating cell lines, a rise of poly(ADP-ribose) as a prerequisite for differentiation was deduced mainly from experiments with inhibitors [5 -81, while in others a transient decrease of poly(ADP-ribose) polymerase activity as a regulatory signal was postulated [9 -121. Conflicting data were presented even for one and the same system: reports on an apparent decrease in poly(ADPribose) polymerase activity during 3T3-LI pre-adipocyte differentiation [13] and an inducing action of polymerase inhibitors [14] contrast with observations of a prevention of differentiation by nicotinamide or benzamide [15]. This led us to analyze the ADP-ribosylation status in vivo during 3T3-Ll

show abstract

Section: Modification Of Hmg Protcinssupporting

confidence: 52%

Differentiation of 3T3‐L1 pre‐adipocytes induced by inhibitors of poly(ADP‐ribose) polymerase and by related noninhibitory acids

Janßen

Hilz

1989

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…More than 2000 million people have been infected with this virus; about 350 millions of whom remain infected chronically and become carriers of the virus [5,6]. Hepatitis C is a small, enveloped, positive stranded RNA virus and is a member of Flaviviridae family [7,8,9]. It can result in acute or chronic hepatitis, 170 million chronic carriers worldwide are present [10,11].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Hepatitis B, C, and D among Thalassemia patients in Sulaimani Governorate

Hama

Sawa

2017

KJAR

View full text Add to dashboard Cite

“…In general, it is considered that the active principle of LPS is a lipid A, which is composed of a disaccharide unit, acylated fatty acids, and phosphates. The ability to produce the Shwartzman reaction appears to require Complete lipid A (11,15), and monosaccharide analogs of lipid A possess pyrogenicity and lethal toxicity (17,24), whereas even compounds with chemical structures different from that of lipid A exhibit mitogenicity and adjuvanticity. It is not known if leptospiral LPS has a part corresponding to the lipid A region, but if it is present, it seems to differ from the chemical structure of lipid A of typical LPS.…”

Section: Discussionmentioning

confidence: 99%

Biological Activities of Lipopolysaccharide‐Like Substance (LLS) Extracted from Leptospira interrogans Serovar canicola Strain Moulton

Shimizu

Matsusaka²,

Nagakura³

et al. 1987

Microbiology and Immunology

View full text Add to dashboard Cite

show abstract

Immunobiological activities of chemically synthesized monosaccharide analogs of lipid A

Cited by 42 publications

References 0 publications

Differentiation of 3T3‐L1 pre‐adipocytes induced by inhibitors of poly(ADP‐ribose) polymerase and by related noninhibitory acids

Differentiation of 3T3‐L1 pre‐adipocytes induced by inhibitors of poly(ADP‐ribose) polymerase and by related noninhibitory acids

Prevalence of Hepatitis B, C, and D among Thalassemia patients in Sulaimani Governorate

Biological Activities of Lipopolysaccharide‐Like Substance (LLS) Extracted from Leptospira interrogans Serovar canicola Strain Moulton

Contact Info

Product

Resources

About