Biological Activities of Lipopolysaccharide‐Like Substance (LLS) Extracted from <i>Leptospira interrogans</i> Serovar <i>canicola</i> Strain Moulton

Shimizu, Tadayori; Matsusaka, Eiichiro; Nagakura, Naotaka; Takayanagi, Kazuhisa; Masuzawa, Toshiyuki; Iwamoto, Yoshihisa; Morita, Tamotsu; Mifuchi, Ichiji; Yanagihara, Yasutake

doi:10.1111/j.1348-0421.1987.tb03134.x

Cited by 34 publications

(31 citation statements)

References 25 publications

(18 reference statements)

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“…Unlike peptidoglycans of other spirochaetes (Umemoto et al, 1981 ;Beck et al, 1990) but in agreement with results obtained for the majority of peptidoglycan preparations (StewartTull, 1980), leptospiral PG was mitogenic for peripheral mononuclear cells. This in citro observation, previously reported also for leptospiral LPS (Shimizu et al, 1987), suggests an in uiuo role for leptospiral PG in activation of the immunocompetent system. In summary, the biological activities of leptospiral PG reported in this study suggest that it plays a role in…”

Section: Discussionmentioning

confidence: 68%

“…However, though several attempts have been made to demonstrate lep t ospiral endo t oxici t y , only limited biological effects were observed in various Leptospira LPS preparations, not sufficient to justify the complex and severe development of the inflammatory response of the host (Arean et al, 1964;Shimizu et al, 1987 al., 1988;De Souza & Koury, 1991). In the present investigation, we describe some relevant biological effects exerted in uitro by PG extracted from a strain of Leptospira interrogans, which are typical of the biologically active peptidoglycans.…”

Section: Discussionmentioning

confidence: 99%

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Biological activity of a peptidoglycan extracted from Leptospira interrogans: in vitro studies

Cinco

Perticarari²,

Presani³

et al. 1993

Journal of General Microbiology

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Peptidoglycan (PG) has been isolated from some species of spirochaetes, including Leptospira interrogans. Although leptospiral PG has been chemically characterized, no study has been carried out on its potential biological activity. Since PG of Treponema and Borreiia is biologically active both in vivo and in vitro, we investigated the capacity of a leptospiral PG preparation to induce relevant biological effects. PG extracted from L. interrogans strain Teramo was mitogenic at 0.1 pg ml-' for human peripheral blood mononuclear cells (PBMC) since it increased the PBMC fraction positive for Ki-67, an antigen expressed by human proliferating cells; at 4 pg ml-', PG was able to induce complement consumption and to stimulate leucocyte phagocytosis and the metabolic burst of resting as well as phagocytosing leucocytes. These findings indicate that Leptospira PG may play a role in modulating the immunocompetent cell functions and suggest that PG can contribute to the host response during Leptospira infection.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Biological activity of a peptidoglycan extracted from Leptospira interrogans: in vitro studies

Cinco

Perticarari²,

Presani³

et al. 1993

Journal of General Microbiology

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show abstract

“…Leptospires have a typical double membrane structure in common with other spirochetes, in which the cytoplasmic membrane and peptidoglycan cell wall are closely associated and are overlain by an outer membrane (254). Leptospiral lipopolysaccharide has a composition similar to that of other gram-negative bacteria (603), but has lower endotoxic activity (519). Leptospires may be stained using carbol fuchsin counterstain (211).…”

Section: Biology Of Leptospiresmentioning

confidence: 99%

Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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“…Other toxic factors, such as LPS, peptidoglycan, and phospholipase, may also play a role in the pathogenesis of leptospirosis, but their roles are not well established (11,15,22,37,44,46). Lipoproteins are also potential toxic factors in leptospirosis as well as in other spirochetal diseases.…”

Section: Vol 70 2002 L Interrogans Glycolipoprotein and Cellular Amentioning

confidence: 99%

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

et al. 2002

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Biological Activities of Lipopolysaccharide‐Like Substance (LLS) Extracted from Leptospira interrogans Serovar canicola Strain Moulton

Abstract: The biological activities of lipopolysaccharide-like substance (LLS)

Cited by 34 publications

References 25 publications

Biological activity of a peptidoglycan extracted from Leptospira interrogans: in vitro studies

Biological activity of a peptidoglycan extracted from Leptospira interrogans: in vitro studies

Leptospirosis

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

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