Immunoassay of taxol and taxol-like compounds in plant extracts

Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

“…Common forms of labeling include radioisotopes (radioimmunoassays, RIA), enzymes (enzyme-linked immunoassays, ELISA), and more recently fluorophores (fluoroimmunoassays, FIA). Both RIA and ELISA have been demonstrated to be effective methods in the detection and measurement of Pac in biological samples (12,13). However, fluorescence monitoring has been shown to be both easier and safe to perform compared to RIA and ELISA.…”

Development of a Competitive Time‐Resolved Fluoroimmunoassay for Paclitaxel

“…We previously tested the effectiveness of immunoassay in quality control of several natural medicines (Fujii et al ., ; Tung et al ., ; Oiso et al ., ; Li et al ., ; Zhao et al ., ); in fact, the earliest attempt of using immunoassay for Tax detection was reported over 20 years ago (Grothaus et al ., , ; Jaziri et al ., ; Leu et al ., , ; Svojanovsky et al ., ). Recently, we successfully prepared an anti‐Tax monocolonal antibody (MAb) 3A3, based on which we established an indirect competitive enzyme‐linked immunosorbent assay (icELISA) for Tax determination with a wide detection range of 9.8 to 312.5 ng/mL, showing obvious advantages over the previously reported methods (Chao et al ., ).…”

Simultaneous LC–MS Analysis of Paclitaxel and Retinoic Acid in Plasma and Tissues from Tumor-Bearing Mice

“…Thus, it is necessary and justified to establish methods for analysis of the two drugs in biological fluids, to study their pharmacokinetics and distribution. Several methods have been reported for analysis of PTX in pharmaceutical samples and biological fluids, including capillary electrophoresis [6], immunoassays [7], liquid chromatography-tandem mass spectrometry (LC-MS-MS) [8] and high-performance liquid chromatography (HPLC) [9]. A literature survey also reveals several methods for assay of ATRA, including HPLC coupled with ultraviolet (UV) [10] and electrochemical [11] detection, liquid chromatography-mass spectrometry (LC-MS) [12,13], and gas chromatography-mass spectrometry (GC-MS) [14].…”