Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Sheikh, Sohail Hamid; Abela, Brain A.; Mulchandani, Ashok

doi:10.1006/abio.2000.4630

Cited by 21 publications

(12 citation statements)

References 18 publications

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“…The development of enzyme-linked immunoassays [111,112] and a fluoroimmunoassay [113] for quantification of paclitaxel in biological samples has been described (SupplemenTary Table 7). These immunoassays utilize the binding of paclitaxel to antibodies for quantification of paclitaxel.…”

Section: Immunoassaysmentioning

confidence: 99%

Quantification of Taxanes in Biological Matrices: A Review of Bioanalytical Assays and Recommendations for Development of New Assays

et al. 2014

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Since the isolation of paclitaxel and its approval for the treatment of breast cancer, various taxanes and taxane formulations have been developed. To date, almost 100 bioanalytical assays have been published with the method development and optimization often extensively discussed by the authors. This Review presents an overview of assays published between January 1970 and September 2013 that described method development and validation of assays used to quantify taxanes in biological matrices such as plasma, urine, feces and tissue samples. For liquid chromatography assays, sample pretreatment, chromatographic separation and assay performance are compared. Since this Review discusses the limitations of previously developed liquid chromatography assays and gives recommendations for future assay development, it can be used as a reference for future development of liquid chromatography assays for the quantification of taxanes in various biological matrices to support preclinical and clinical studies.

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Section: Immunoassaysmentioning

confidence: 99%

Quantification of Taxanes in Biological Matrices: A Review of Bioanalytical Assays and Recommendations for Development of New Assays

et al. 2014

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“…Tubulin‐based biochemical assay is characterized by a minimal pre‐treatment of sample but its sensitivity is limited compared to LC‐UV and LC‐MS methods (Hamel et al, ; Morais et al, ). Even if immunoassays (Grothaus et al, ; Svojanovsky et al, ; Sheikh et al, ) are characterized by a good LOQ (below 0.5 ng/mL), this method could be invalidated by cross reactivity of metabolites to antibodies resulting in decreasing of concentration accuracy. MEKC combines chromatographic and electrophoretic separation principles and the separation is based on the differential partitioning of an analyte between the two‐phase system: the mobile aqueous phase and micellar pseudostationary phase.…”

Section: Classes Of Anticancer Natural Productsmentioning

confidence: 99%

Mass spectrometry in the pharmacokinetic studies of anticancer natural products

Crotti

Posocco

Marangon

et al. 2015

Mass Spectrometry Reviews

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In the history of medicine, nature has represented the main source of medical products. Indeed, the therapeutic use of plants certainly goes back to the Sumerian and Hippocrates and nowadays nature still represents the major source for new drugs discovery. Moreover, in the cancer treatment, drugs are either natural compounds or have been developed from naturally occurring parent compounds firstly isolated from plants and microbes from terrestrial and marine environment. A critical element of an anticancer drug is represented by its severe toxicities and, after administration, the drug concentrations have to remain in an appropriate range to be effective. Anyway, the drug dosage defined during the clinical studies could be inappropriate for an individual patient due to differences in drug absorption, metabolism and excretion. For this reason, personalized medicine, based on therapeutic drug monitoring (TDM), represents one of most important challenges in cancer therapy. Mass spectrometry sensitivity, specificity and fastness lead to elect this technique as the Golden Standard for pharmacokinetics and drug metabolism studies therefore for TDM. This review focuses on the mass spectrometry-based methods developed for pharmacokinetic quantification in human plasma of anticancer drugs derived from natural sources and already used in clinical practice. Particular emphasis was placed both on the pre-analytical and analytical steps, such as: sample preparation procedures, sample size required by the analysis and the limit of quantification of drugs and metabolites to give some insights on the clinical practice applicability. © 2015 Wiley Periodicals, Inc. Mass Spec Rev. 36:213-251, 2017.

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“…Reports have demonstrated that a capillary immunosensor can be utilized for the analysis of heart marker proteins: myoglobin, creatine kinase mb, troponin I, and fatty acid-binding protein, 18,19 and for the measurement of paclitaxel, an anticancer drug. 20,21 However, most capillary immunosensors are used primarily in automated systems, they therefore require a flow-through process with reagents on the sub-milliliter order. Thus, even in the capillary immunosensors, they consume large amounts of expensive reagents and precious samples.…”

Section: Introductionmentioning

confidence: 99%

Single-Step Sandwich Immunoreaction in a Square Glass Capillary Immobilizing Capture and Enzyme-linked Antibodies for Simplified Enzyme-linked Immunosorbent Assay

et al. 2012

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To simplify the complicated operation steps and to minimize sample and reagent amounts for enzyme-linked immunosorbent assays (ELISA), we developed a square glass capillary immunosensor containing both covalently immobilized capture antibodies and physically adsorbed enzyme-linked antibodies. The immobilization of capture antibodies (anti-human IgG) was carried out by the treatment of 3-aminopropyltriethoxy silane, glutaraldehyde, and protein-A, followed by affinity capture of the antibody. In contrast, the enzyme-linked antibodies (alkaline phosphatase (ALP)-linked anti-human IgG) were physically adsorbed on the four corners of the capillary with the aid of polyethylene glycol (PEG) acting as a scaffold. A nanoliter volume of antigen (human IgG)-containing sample solution was introduced via capillary action. This addition resulted in the release and diffusion of ALP-linked anti-human IgG into the bulk solution. This event led to a 20-min single-step sandwich immunoreaction at the inner wall of capillary; the reaction was detected through the reaction with fluorescein diphosphate (FDP) which generated a fluorescent product, fluorescein. Using this technique, we obtained an intra-capillary precision with a coefficient of variation of 9.7%. In addition, the specificity study showed that the human IgG capillary immunosensor did not respond to rabbit IgG. Quantitative analysis was possible within the response range of 10 - 5000 ng mL(-1) anti-human IgG. This capillary immunosensor can act as a single analytical unit or can be integrated into a capillary array for multiple bioanalysis.

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Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Cited by 21 publications

References 18 publications

Quantification of Taxanes in Biological Matrices: A Review of Bioanalytical Assays and Recommendations for Development of New Assays

Quantification of Taxanes in Biological Matrices: A Review of Bioanalytical Assays and Recommendations for Development of New Assays

Mass spectrometry in the pharmacokinetic studies of anticancer natural products

Single-Step Sandwich Immunoreaction in a Square Glass Capillary Immobilizing Capture and Enzyme-linked Antibodies for Simplified Enzyme-linked Immunosorbent Assay

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