Immunoarchitecture of Regenerated Splenic and Lymph Node Transplants

Pabst, Reinhard; Westermann, Jürgen; Rothkötter, H. J.

doi:10.1016/s0074-7696(08)60500-8

Cited by 54 publications

(55 citation statements)

References 161 publications

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“…As this occurs, the deformable portion of the cell bends to negotiate the opening, whereas the inclusion, which is nondeformable, is unable to pass through the narrow passage; thus, it is left behind to be phagocytized by resident macrophages. [23][24][25] The above considerations led us to adapt the protocol developed by Hayashi et al 6 to measure the spleen phagocytic activity. Our results support the view that the antimutagenic action toward MNRET in peripheral blood can be modulated largely by the splenic phagocytic activity (Table 4), which is equally increased with L. edodes treatment both at 6 and 12 h (P < .001) after micronucleus induction by CP.…”

Section: Discussionmentioning

confidence: 99%

Lentinula edodes (Shiitake) Modulates Chemically Induced Mutagenesis by Enhancing Pitting

Lima

Sugui

Petrício

et al. 2013

Journal of Medicinal Food

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This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of -20°C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at -20°C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells.

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Section: Discussionmentioning

confidence: 99%

Lentinula edodes (Shiitake) Modulates Chemically Induced Mutagenesis by Enhancing Pitting

Lima

Sugui

Petrício

et al. 2013

Journal of Medicinal Food

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“…3,7 The animals were kept under barrier-maintained conditions for 3 months before analysis to allow recovery from the operation and adjustment to the asplenic situation. Sham splenectomy was performed as described above with the exception that after cutting the splenic ligaments the spleen was put back into the abdominal cavity.…”

Section: Splenectomymentioning

confidence: 99%

“…3,31 Although red pulp and follicles of such splenic transplants are well developed 31 and functional, 2,32 a marked B lymphocytosis is still observed in these animals, 2,7 suggesting that red pulp and follicles are not involved in LFA-1 and ICAM-1 regulation on B cells. In contrast, the MZ is poorly developed 3,31,33 and not functional as shown by the increased susceptibility to infections. 33 This suggests a role of the MZ in the maintenance of LFA-1 and ICAM-1 expression on B cells in blood and lymph nodes.…”

Section: Spleen Affects B-cell Adhesion Molecule Expression 3039mentioning

confidence: 99%

“…1,2 On removal of the spleen the incidence of severe infections increases both in children and adults, 3,4 and there are changes in many immunologic parameters. 5 One lasting change is a high increase in the number of B lymphocytes in the blood, an effect that is constantly observed both in humans 6 and in experimental animals, 7,8 and that comparably affects the 2 compartments of the blood, the peripheral and the marginal pools.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Splenectomy of rats selectively reduces lymphocyte function-associated antigen 1 and intercellular adhesion molecule 1 expression on B-cell subsets in blood and lymph nodes

Milićević

Luettig

Wüstefeld

et al. 2001

Blood

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IntroductionAntigens circulating in the blood are retained in the spleen and presented to a huge number of B and T cells migrating through its different compartments. 1,2 On removal of the spleen the incidence of severe infections increases both in children and adults, 3,4 and there are changes in many immunologic parameters. 5 One lasting change is a high increase in the number of B lymphocytes in the blood, an effect that is constantly observed both in humans 6 and in experimental animals, 7,8 and that comparably affects the 2 compartments of the blood, the peripheral and the marginal pools. 9 Because it is not known whether all or only certain blood B-cell subsets increase in number after the loss of the spleen, the numbers of naïve (IgD ϩ IgM ϩ ), memory (IgD Ϫ IgM high ), newly formed (NF; IgM high CD90 high ), early recirculating follicular (ERF; IgM low CD90 high ), recirculating follicular (RF; IgM low CD90 Ϫ ), and marginal zone (MZ; IgM high CD90 Ϫ ) phenotype B cells 10 were determined in blood, lymph nodes, and bone marrow of control and splenectomized animals. In principle, the splenectomy-induced increase in blood B-cell numbers could be due to an altered migration pattern or to increased proliferation of B cells caused, for example, by a latent infection.Because migration and proliferation of B cells are regulated by surface molecules, our hypothesis was that their expression might be altered by splenectomy. Therefore, the expression of molecules involved in initial adhesion of lymphocytes to the endothelium, in transmigration through the endothelium and the tissue, and in B-cell activation within the tissue was analyzed in all B-cell subsets in blood, lymph nodes, and bone marrow (␣ 4 -integrins, CD44, L-selectin, lymphocyte function-associated antigen1 [11][12][13][14] ). The functional consequences of possible changes in surface molecule expression were tested in vitro by adhesion assays and in vivo by following the traffic of labeled B cells from blood to thoracic duct lymph. In addition, the number of proliferating B cells was determined in the bone marrow, in the B-cell area of lymph nodes, and in the blood.Our data show that loss of the spleen leads to a decrease in LFA-1 and ICAM-1 expression of B cells but not T cells in blood and lymph nodes. This accelerates the migration of B cells through lymph nodes and results in higher numbers of B cells in the thoracic duct lymph and, subsequently, in the blood. Materials and methods AnimalsNormal rats (LEW/Ztm), rats with high numbers of B cells (BH.1L/Won 15 ), and rats lacking T cells (LEW/Ztm-rnu 16 ) were obtained from the Central Animal Facility at the Medical School of Hannover and were barriermaintained in a room with controlled environment (22 Ϯ 2°C, 55% Ϯ 5% relative humidity, 12-hour light/dark cycle). SplenectomySplenectomy was performed as described previously. 3,7 The animals were kept under barrier-maintained conditions for 3 months before analysis to allow recovery from the operation and adjustment to the asplenic situation. Sh...

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“…Splenotic tissue has a decreased amount of white pulp, 51,52 and while it contains many elements of the normal spleen, the splenic nodules are smaller in mass with reduced individual compartments. 47 The reduced immune function of these ectopic implants may be partially related to the decreased amount of white pulp.…”

Section: 87-90mentioning

confidence: 99%