Abstract:This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results ind… Show more
“…This observation may be explained by comparing results from micronuclei and splenic phagocytosis, which showed increased phagocytic activity in spleens from C. adamantium-treated mice. It is known that the spleen removes defective cells from the bloodstream, including micronucleated cells (de Lima et al, 2013). Therefore, this finding supports the hypothesis that the extract is capable of inducing cellular DNA damage.…”
Section: Discussionsupporting
confidence: 84%
“…For each animal, 200 cells were analyzed using a fluorescence microscope at 400X magnification, a 420-490-nm excitation wavelength, and a 520-nm barrier filter. The analysis was performed as previously described by Hayashi et al (1990) and adapted by de Lima et al (2013).…”
“…This observation may be explained by comparing results from micronuclei and splenic phagocytosis, which showed increased phagocytic activity in spleens from C. adamantium-treated mice. It is known that the spleen removes defective cells from the bloodstream, including micronucleated cells (de Lima et al, 2013). Therefore, this finding supports the hypothesis that the extract is capable of inducing cellular DNA damage.…”
Section: Discussionsupporting
confidence: 84%
“…For each animal, 200 cells were analyzed using a fluorescence microscope at 400X magnification, a 420-490-nm excitation wavelength, and a 520-nm barrier filter. The analysis was performed as previously described by Hayashi et al (1990) and adapted by de Lima et al (2013).…”
“…In addition, animals pre-treated with extract prepared with powder stored at -20 ºC exhibited increased basal DNA damage when compared with the untreated control animals, demonstrating that storage can alter the biological properties of bio-compounds and, in such a case, result in genotoxic effects (LIMA et al, 2010). The same research group demonstrated that L. edodes extracts facilitate the removal of circulating erythrocytes with intraerythrocytic inclusions, such as micronuclei and protozoa, from phagocytosis by splenic macrophages, in a process known as pitting (LIMA et al, 2013).…”
Section: The Biological Effect Of Natural Products Obtained From Micr...mentioning
confidence: 97%
“…Polysaccharides obtained from edible mushrooms, such as Lentinus edodes (shiitake) and Agaricus brasiliensis (sun mushroom), are also widely studied for their biological effects (LESSNA et al, 2015;LIMA et al, 2010;LIMA et al, 2013;MARTINS et al, 2008). Martins et al (2008) reported that the polysaccharide-rich fraction of A. brasiliensis increased the microbicidal activity of macrophages against Candida albicans, stimulating hydrogen peroxide production and mannose receptor expression by peritoneal macrophages, which is important for non-opsonized microorganism attachment and phagocytosis.…”
Section: The Biological Effect Of Natural Products Obtained From Micr...mentioning
Bioactive compounds present in plants, microorganisms, and animals are the subject of scientific research, mainly focusing on the discovery of novel pharmacologically active molecules for application in human and veterinary medicine, as well as agriculture. Brazil is the country with the greatest biodiversity globally, accounting for 15–20% of global diversity. Therefore, the objective of the present study was to carry out a bibliographic survey on studies involving natural compounds conducted by staff of the Federal University of Mato Grosso, Sinop campus. The review included scientific articles published in specialized journals by the staff from the moment they were hired, according to information in their Lattes Curriculum. During the survey period, the campus had 314 staff, and 311 scientific articles were published. Among the 311 articles, 122 studies met the inclusion criteria and were selected for this literature review. According to the findings, UFMT – Sinop has contributed to research in the field of natural products, carrying out studies on the biological effects of compounds present in extracts obtained from plants, microorganisms, and animal secretions.
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