Immunoanalytical Technique: Enzyme-Linked Immunosorbent Assay (ELISA)

González-Martı́nez, Miguel Ángel; Puchades, Rosa; Maquieira, Ángel

doi:10.1016/b978-0-12-814264-6.00015-3

Cited by 15 publications

(16 citation statements)

References 134 publications

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“…An enzyme bound with a substrate, a fluorescent molecule, or a radioactive isotope can be described as the tag [14]. ELISA has been used in various fields such as environmental studies in order to detect pollutants, food industry in order to detect toxins or allergens, and medical studies in order to detect pharmaceuticals, disease markers [22]. When we approach ELISA in terms of viruses, it can provide the detection of a virus or an antibody from a sample of a patient infected by viruses [14].…”

Section: Chapter Outlinementioning

confidence: 99%

“…The quantity of viruses from a cell culture/sample or a specific antibody to a specific viral pathogen can be detected using ELISA [14]. Those studies are very crucial for diagnostics [22]. ELISA is a much faster method than immunoblotting to detect a specific viral protein.…”

Section: Chapter Outlinementioning

confidence: 99%

“…On the other hand, ELISA tests can sometimes be quite expensive due to the cost of reagents being used [23]. Direct ELISA, indirect ELISA, sandwich ELISA, and competitive ELISA are the different types of ELISA tests which are used for different scientific fields [14,21,22].…”

Section: Chapter Outlinementioning

confidence: 99%

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Section: Chapter Outlinementioning

confidence: 99%

Section: Chapter Outlinementioning

confidence: 99%

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Electrochemical virus detections with nanobiosensors

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“…Immunometric methods find many applications in diverse scientific fields, e.g., agriculture, environmental protection, veterinary science, medicine, food analysis, and molecular biology. Applications range from the analysis of compounds such as proteins and peptides, microorganisms (molds, bacteria), toxins (mold, bacterial, and other), hormones, antibiotics, vitamins, pesticides, and metal ions to small particles such as nucleotides, DNA or RNA [35][36][37].…”

Section: Theoretical Basics Of Immunoassaysmentioning

confidence: 99%

Review: immunoassays in DNA damage and instability detection

Boguszewska

Szewczuk

Urbaniak

et al. 2019

Cell. Mol. Life Sci.

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The review includes information on the current state of knowledge of immunometric methods with emphasis on the possibility of deoxyribonucleic acid (DNA) damage detection. Beginning with basic immunoassay enzyme-linked immunosorbent assay (ELISA), this review describes methods such as tyramide signal amplification (TSA), enhanced polymer one-step staining (EPOS), and time resolved amplified cryptate emission (TRACE) as improvements of ELISA's developed over time to obtain more accurate results. In the second part of the review, surface plasmon resonance (SPR) and quantum dots (QDs) are presented as the newest outlooks in the context of immunoanalysis of biological material and molecular studies. The aim of this review is to briefly present immunoassays with emphasis on DNA damage detection; therefore, the types of methods are listed and described, types of signal indicators, basic definitions such as antigen and antibody are given. Every method is considered with an exemplary application focusing on DNA studies, DNA damage and instability detection.

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“…The majority of these methods are inexpensive, easy to handle, sensitive enough to determine trace amounts of the analyte, and suitable for a high-throughput analysis of a large number of samples. Because of these advantages, these methods are used in food safety and authentication analysis [1,2], the monitoring of environmental contamination and diseases markers [3,4], forensic examinations, and exploratory research screening [5,6]. The significant variety and abundance of natural and synthetic substances, used as target analytes, encourage the development of multidetection methods.…”

Section: Introductionmentioning

confidence: 99%

Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten

2019

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Conjugation chemistry does not always provide adequate spatial orientation of hapten in immunogens for the best presentation of generic or individual epitopes. In the present study, the influence of unique and multiple orientations of immunizing hapten on the immune response repertoire was compared to select generic recognition system. The glycopeptides, teicoplanin (TPL) and ristomycin (RSM), were conjugated to BSA to produce immunogens with unique and multiple orientations of haptens. Polyclonal antibodies generated against TPL conjugated through a single site were of uniform specificity and demonstrated selective TPL recognition, regardless of the coating conjugates design. The sensitivity (IC50) of 4 enzyme-linked immunosorbent assays (ELISAs) for TPL varied little within the 3.5–7.4 ng/mL, with a dynamic range of 0.2–100 ng/mL. RSM was coupled to BSA through several glycoside sites that evoked a wider repertoire of response. This first described anti-RSM antibody was selective for RSM in homologous hapten-coated ELISAs with IC50 values in the range 4.2–35 ng/mL. Among the heterologous antigens, periodate-oxidized TPL conjugated to gelatine was selected as the best binder of generic anti-RSM fraction. The developed ELISA showed group recognition of glycopeptides RSM, TPL, eremomycin, and vancomycin with cross-reactivity of 37–100% and a 10–10,000 ng/mL dynamic range. Thus, multiple presentations of immunizing hapten help expand the repertoire of immune responses and opportunities for the selection of the required fine-specificity agent.

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Immunoanalytical Technique: Enzyme-Linked Immunosorbent Assay (ELISA)

Cited by 15 publications

References 134 publications

Electrochemical virus detections with nanobiosensors

Electrochemical virus detections with nanobiosensors

Review: immunoassays in DNA damage and instability detection

Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten

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