Immunoaffinity pre-concentration combined with on-column visual detection as a tool for rapid aflatoxin M1 screening in milk

Rapid screening test for adulteration in raw materials of dietary supplements

“…This equates or corresponds to a 0.5% error rate. An error rate of less than 1% is acceptable for this test [19][20][21].…”

Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk

“…The diluted milk samples were added directly into the column based immunoassay in previous research. (Goryacheva et al 2009) Milk is a very complicated matrix: it contains many macromolecular substances (proteins, sugars and lipids, among others). These macromolecular substances could be retained in the gel layers and lead to obtaining of false results.…”

Occurrence of Aflatoxin M1 in Dairy Products

“…A nice approach for the rapid detection of AFM1 in milk, which exploits components of an immunoassay, carried out "on-column" instead of in a microtitre plate, was proposed by Sibanda et al (1999). This visual assay has been extended and applied to yogurt and kefir by Goryacheva et al (2009). Briefly, a specific antibody is immobilized on a gel-support, which is packed into a cartridge to form an immune-layer.…”