Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk

Jiang, Wenxiao; Beloglazova, Natalia V.; Wang, Zhanhui; Jiang, Haiyang; Wen, Kai; Saeger, Sarah De; Luo, Pengjie; Wu, Yongning; Shen, Jianzhong

doi:10.1016/j.bios.2014.11.004

Cited by 64 publications

(39 citation statements)

References 23 publications

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“…There have been many applications of the sequential injection method for measuring drugs, proteins and other targets [8,62,[68][69][70][71][72]. For instance, nonlabeled HSA was used as both the target and 'label' in an HPLCbased sequential injection method that could measure this protein in samples in less than 10 min [68].…”

Section: Sequential Injection Methodsmentioning

confidence: 99%

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Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

et al. 2015

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Section: Sequential Injection Methodsmentioning

confidence: 99%

“…This method has been used with liposome labels and fluorescence detection for the measurement of IgE in serum [71]. It has further been employed with a label based on liposome-encapsulated quantum dots in a multiplex assay for a variety of sulfonamides and quinoline residues in milk [72].…”

Section: Sequential Injection Methodsmentioning

confidence: 99%

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

et al. 2015

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“…15 To date, no immunoassays have been established for detecting residues of 1 in foods. Recently, immunoassays, especially the ELISA, have proven to be an efficient method for the analysis of numerous samples in a timely way.…”

Section: ■ Introductionmentioning

confidence: 99%

Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay

Jiang

Beier

Luo

et al. 2015

J. Agric. Food Chem.

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Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin-streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC50 value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk, and 2.75 μg/kg in honey. The average recovery of the BA-ELISA ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.

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“…These methods can offer accurate, sensitive, and specific analysis for SA contaminates in various matrices including meats, eggs, milk, and so on. However, instrumental methods (Adrian et al 2009;Jiang et al 2014) such as high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS) not only need expensive instrumentations but also require a complex sample pretreatment procedure to achieve the extraction of the analyte and the purification of the matrix. The well-established biochemical immunoassay such as ELISA has unachievable shortcomings in the automation analysis and requires skilled operators (Mohseni et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor

Pan

Wang

et al. 2016

Food Anal. Methods

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In this research, a reproducible surface plasmon resonance (SPR) immunosensor based on inhibition format was fabricated for stable and sensitive detection of four sulfonamides (SAs) in animal-derived foods. The parameters in the fabrication and measurement process were optimized and discussed in details. The method using the proposed SPR immunosensor was validated and exhibited favorable performance for SAs residues detection in common animal-derived food products, as well as acceptable accuracy (pure milk 88.5-106.2%; egg 89.0-91.5%; chicken muscle 87.2-94.7%; beef 84.8-96.9%; fish 87.8-95.0%), precision (relative standard deviation (n = 3), pure milk 3.2-5.2%; egg 1.1-5.4%; chicken muscle 1.1-5.9%; beef 2.5-7.1%; fish 4.6-5.3%), and sensitivity (IC 15 , pure milk 59.6 ng mL − 1 , egg 56.1 ng mL − 1 , chicken muscle 66.9 ng mL −1 , beef 63.3 ng mL −1 , fish 62.7 ng mL −1 ). Each detection cycle could finish in less than 5 min, and each SPR chip could reuse 300 analysis cycles with response attenuation of 3.7%. These results have demonstrated that the proposed SPR immunosensor has offered an effective, accurate, sensitive, rapid, and low-cost methodology for SAs residue detection in food sample, and this method has great potential for the routine analysis of large numbers of samples on measuring different kinds of compounds.

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Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk

Cited by 64 publications

References 23 publications

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay

Stable and Sensitive Detection of Sulfonamide Residues in Animal-Derived Foods Using a Reproducible Surface Plasmon Resonance Immunosensor

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