Immunoaffinity Enrichment Lc–Ms/Ms Quantitation Ofcdh17 in Tissues

Caporuscio, Christian; Holmes, Derek; Olah, Timothy; Shipkova, Petia

doi:10.4155/bio-2020-0162

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…The composition of the ECs de nes cadherin protein subfamilies. CDH17 is a structurally unique cadherin that differs from classical cadherins including E-, N-, and P-cadherins whereby CDH17 comprises of seven, instead of ve, extracellular cadherin repeats (EC1-EC7), and its cytoplasmic domain lacks homology with other cadherin proteins [142]. In normal cells, CDH17 is almost exclusively expressed in the epithelium of the colon, small intestine and duodenum.…”

Section: Discussionmentioning

confidence: 99%

CDH17 as a Cell Surface Immunotherapy Target in Colorectal Cancer: An In Silico Analysis

Wong

2022

Preprint

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Immunotherapy development against colorectal cancer (CRC) is hindered by the lack of surface antigen highly expressed in cancer cells but with restricted presence in normal tissues to minimize off-tumor toxicities. In this in silico analysis, a longlist of genes (n=13,488) expressed in CRCs according to the Human Protein Atlas (HPA) database were evaluated to shortlist for potential surface targets based on the following prerequisites: (i) Absent from the brain and lung tissues to minimize the likelihood of neurologic and pulmonary toxicities; (ii) Restricted expression profile in other normal human tissues; (iii) Genes that potentially encode cell surface proteins and; (iv) At least moderately expressed in CRC cases. Fifteen potential targets were shortlisted and subsequently ranked according to the combination of their transcript and protein expression levels in CRCs derived from multiple datasets (i.e. DepMap, TCGA, CPTAC-2, and HPA CRCs). The top-ranked target with the highest and homogenous expression in CRCs was cadherin 17 (CDH17). Downstream in silico analyses in CRC cases showed that CDH17 was highly correlated with carcinoembryonic antigen expression, CDH17 expression was lower in cases with high microsatellite instability, as well as negatively associated with the expression of MHC class I and II molecules. In summary, CDH17 represents an optimal target for immunotherapy development against CRCs, and this study provides a framework to shortlist for promising surface targets for immunotherapy development against other malignancies.

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Section: Discussionmentioning

confidence: 99%

CDH17 as a Cell Surface Immunotherapy Target in Colorectal Cancer: An In Silico Analysis

Wong

2022

Preprint

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Integrated transcriptomics and proteomics data analysis identifies CDH17 as a key cell surface target in colorectal cancer

Wong

2023

Computational Biology and Chemistry

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Quantitative Bioanalysis of Proteins by Digestion and LC–MS/MS: The Use of Multiple Signature Peptides

Sleumer,

Kema,

van de Merbel

2023

Bioanalysis

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The use of multiple signature peptides for the quantification of proteins by digestion and LC–MS/MS is reviewed and evaluated here. A distinction is made based on the purpose of the use of multiple peptides: confirmation of the protein concentration, discrimination between different protein forms or species and in vivo biotransformation. Most reports that describe methods with at least two peptides use these for confirmation, but it is not always mentioned how the peptides are used and how possible differences in concentration between the peptides are handled. Differences in concentration are often reported in the case of monitoring different protein forms or in vivo biotransformation, and this offers insight into the biological fate of the protein.

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Immunoaffinity Enrichment Lc–Ms/Ms Quantitation Ofcdh17 in Tissues

Cited by 3 publications

References 13 publications

CDH17 as a Cell Surface Immunotherapy Target in Colorectal Cancer: An In Silico Analysis

CDH17 as a Cell Surface Immunotherapy Target in Colorectal Cancer: An In Silico Analysis

Integrated transcriptomics and proteomics data analysis identifies CDH17 as a key cell surface target in colorectal cancer

Quantitative Bioanalysis of Proteins by Digestion and LC–MS/MS: The Use of Multiple Signature Peptides

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