Immunoaffinity chromatography of lymphocyte subpopulations using tert-amine derived matrices with adsorbed antibodies

Kataoka, Kazunori; Sakurai, Yasuhisa; Hanai, Tetsuro; Maruyama, Atsushi; Tsuruta, Teiji

doi:10.1016/0142-9612(88)90087-7

Cited by 33 publications

(15 citation statements)

References 14 publications

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“…Various cell separation methods have been developed. [ 7–13 ] Flow cytometry by labeling cells with fluorescent antibodies or magnetic particles is used as a precise cell separation method. However, cell modification with fluorescent antibodies or magnetic particles may affect the cells’ intrinsic properties and cause unexpected reactions in the human body after transplantation.…”

Section: Figurementioning

confidence: 99%

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Nagase

Ota

Hirotani

et al. 2020

Macromol. Rapid Commun.

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Recently, cell separation methods have become important for preparing cells for transplantation therapy. In this study, a thermoresponsive cationic block copolymer brush is developed as an effective cell separation tool. This brush is prepared on glass surfaces using two steps of activator regenerated by electron transfer–atom transfer radical polymerization (ARGET‐ATRP). The cationic segment is prepared in the first step of the ARGET‐ATRP of N,N‐dimethylaminopropylacrylamide (DMAPAAm). In the second step, the thermoresponsive segment is prepared, attached to the bottom cationic segment, through ARGET‐ATRP with N‐isopropylacrylamide (NIPAAm). The cell adhesion behavior of the prepared thermoresponsive cationic copolymer, PDMAPAAm‐b‐PNIPAAm, brush is observed using umbilical cord‐derived mesenchymal stem cells (UCMSC), fibroblasts, and macrophages. At 37 °C, all three types of cells adhere to the thermoresponsive cationic copolymer brush. Then, by reducing the temperature to 20 °C, the adhered UCMSC are detached from the copolymer brush, whereas the fibroblasts and macrophages remain adhered to the copolymer brush. Using this copolymer brush, UCMSC can be purified from the cell mixture simply by changing the temperature. Therefore, the prepared thermoresponsive cationic copolymer brush is useful as a cell separation tool for the purification of mesenchymal stem cells.

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Section: Figurementioning

confidence: 99%

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Nagase

Ota

Hirotani

et al. 2020

Macromol. Rapid Commun.

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show abstract

“…Cell separation is a key technology in the isolation of cells from tissue and the transplantation of blood cells or mesenchymal stem cells. Techniques such as centrifugation,1–3 fluorescence‐activated cell sorting (FACS),4 magnetic cell selection,5–8 affinity column chromatography,9–11 and membrane filtration12–17 are typically employed for cell separation. The centrifugal separation of cells is a typical method employed to separate platelets, leukocytes, mononuclear cells, red blood cells (RBCs), and nonblood cells.…”

Section: Introductionmentioning

confidence: 99%

Separation of CD34⁺ cells from human peripheral blood through polyurethane foaming membranes

Higuchi

Iizuka

Gomei

et al. 2006

J Biomedical Materials Res

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Cell separation from peripheral blood was investigated using polyurethane (PU) foaming membranes and PU membranes (pore size, 5 or 12 mum) at different blood permeation speeds. Permeation ratio of hematopoietic stem cells (CD34(+) cells) through the PU membranes was the lowest among the blood cells at any blood permeation speed. This is thought to be because CD34(+) cells are more adhesive than red blood cells (RBCs), platelets, T cells, and B cells. Primitive hematopoietic stem and progenitor cells tend to adhere to the surface of mature blood cells, because of the high expression of cell-adhesion molecules on the surface of the cells. Human serum albumin solution was exposed to PU-COOH membranes to detach adhered cells from the surface of the membranes, allowing isolation of CD34(+) cells and reduction of RBCs in the permeate solution. Most purified CD34(+) cells (high recovery ratio of CD34(+) cells divided by recovery ratio of RBCs) were obtained in the recovery process using PU-COOH membranes (pore size, 5.2 microm) at a permeation speed of 0.3-1 mL/min.

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“…Thus, a method is needed for the separation and isolation of specific mesenchymal cells from bone‐marrow stromal cells. Cell separation can be accomplished by centrifugation,3, 4 fluorescence‐activated cell sorting (FACS),5 magnetic cell selection,6–9 affinity chromatography,10, 11 or membrane filtration 12–14. Of these methods, membrane filtration method is a good candidate for the purification of mesenchymal cells because it is simple and inexpensive and because it is easy to maintain sterility during the filtration process.…”

Section: Introductionmentioning

confidence: 99%

Cell separation between mesenchymal progenitor cells through porous polymeric membranes

et al. 2005

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This study investigates the separation of two types of marrow stromal cells, KUSA-A1 osteoblasts and H-1/A preadipocytes, by filtration through various porous polymeric membranes. It was found that KUSA-A1 permeates better than H-1/A cells through 12-microm polyurethane foaming membranes. This appears to be due to the relatively smaller cell size of KUSA-A1 cells. In addition, when feed solutions containing suspensions of either cell type or a mixture of the two were used, the permeation ratio was relatively low (< 6%) through polyurethane and surface-modified polyurethane foaming membranes. It was also found that there was some degree of separation between KUSA-A1 and H-1/A cells (separation factor = 1.8) with nylon-net filter membranes, but no separation was obtained when filters made of nonwoven fabrics or silk screens were used. This ability of the nylon-net filter membranes to separate the two cell types was due to a sieving effect that results from an optimal pore size. Finally, permeation of a solution of human serum albumin through the membrane following filtration of the cells did not result in a separation of cells in the recovery solution.

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Immunoaffinity chromatography of lymphocyte subpopulations using tert-amine derived matrices with adsorbed antibodies

Cited by 33 publications

References 14 publications

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Separation of CD34⁺ cells from human peripheral blood through polyurethane foaming membranes

Cell separation between mesenchymal progenitor cells through porous polymeric membranes

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Immunoaffinity chromatography of lymphocyte subpopulations using tert-amine derived matrices with adsorbed antibodies

Cited by 33 publications

References 14 publications

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Thermoresponsive Cationic Block Copolymer Brushes for Temperature‐Modulated Stem Cell Separation

Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes

Cell separation between mesenchymal progenitor cells through porous polymeric membranes

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Separation of CD34⁺ cells from human peripheral blood through polyurethane foaming membranes