Cell separation between mesenchymal progenitor cells through porous polymeric membranes

Abstract

This study investigates the separation of two types of marrow stromal cells, KUSA-A1 osteoblasts and H-1/A preadipocytes, by filtration through various porous polymeric membranes. It was found that KUSA-A1 permeates better than H-1/A cells through 12-microm polyurethane foaming membranes. This appears to be due to the relatively smaller cell size of KUSA-A1 cells. In addition, when feed solutions containing suspensions of either cell type or a mixture of the two were used, the permeation ratio was relatively low (< 6%) through polyurethane and surface-modified polyurethane foaming membranes. It was also found that there was some degree of separation between KUSA-A1 and H-1/A cells (separation factor = 1.8) with nylon-net filter membranes, but no separation was obtained when filters made of nonwoven fabrics or silk screens were used. This ability of the nylon-net filter membranes to separate the two cell types was due to a sieving effect that results from an optimal pore size. Finally, permeation of a solution of human serum albumin through the membrane following filtration of the cells did not result in a separation of cells in the recovery solution.

Synthesis and performance of amphiphilic copolymers for blood cell separation

“…Cell separation is a key process involved in leukocyte removal from blood cells for transfusion1–5 and isolation of stem cells from peripheral and umbilical cord blood 6, 7. Polyurethane foam membranes1–3, 6, 7 and nonwoven fabrics,4 with pore sizes of 5–20 μm, have been used as separation membranes for blood cells.…”

“…The mechanism of cell separation through the membranes has yet to be clarified. However, it is generally assumed that the separation mechanism is based on the following: (a) the sieving of cells through the porous membranes, (b) the interactions (e.g., surface tension, charge density) between cells and the membrane surface, and (c) the adhesion of cells to the membrane surface by a biological process 7, 8…”

Permeation of blood cells from umbilical cord blood through surface-modified polyurethane foaming membranes

“…Centrifugation [1,2], affinity column chromatography [3,4], fluorescence activated cell sorting (FACS) [5,6], magnetic cell selection (MACS) [7,8], and membrane filtration [9][10][11][12][13][14] are techniques typically employed for cell separation. Highly purified cellular preparations are obtained using Ficoll-Paque method followed by FACS or MACS in conjunction with a fluorescently labeled antibody as the cell-surface marker, while the cell separation by membrane filtration method is less specific, but is simple and easy operation, and sterility can maintain during the process.…”

Separation of CD34+ cells from human peripheral blood through polyurethane foaming membranes

“…Cell separation is a key technology in the isolation of cells from tissue and the transplantation of blood cells or mesenchymal stem cells. Techniques such as centrifugation,1–3 fluorescence‐activated cell sorting (FACS),4 magnetic cell selection,5–8 affinity column chromatography,9–11 and membrane filtration12–17 are typically employed for cell separation. The centrifugal separation of cells is a typical method employed to separate platelets, leukocytes, mononuclear cells, red blood cells (RBCs), and nonblood cells.…”