Immunoactivity of polymer microspheres with their hydrophilic/hydrophobic heterogeneous surface sensitized with an antibody

Adsorption of immunogamma globulin onto various synthetic calcium hydroxyapatite particles

“…These proteins, naturally designed with extraordinary specificity and binding affinity for a given antigen, are used as diagnostic reagents. Therefore, many investigations have been done using different adsorbents such as silica gel [4,5], polymer latex [6][7][8], and Teflon [9,10] particles. In the development of diagnostic reagents, the main goal is to achieve stable attachment of IgG molecules at the solid surfaces without disrupting or masking the biological site binding character for proteins.…”

“…IgG or antibodies are proteins synthesized by an animal in response to the presence of a foreign substance called an antigen [1][2][3]. These proteins, naturally designed with extraordinary specificity and binding affinity for a given antigen, are used as diagnostic reagents.…”

Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal‐Based Rapid Diagnostic

“…On comparing with the hydrophobic latex particle coated with the same antibody, it was found that the functionalized latex presented low nonspecific interaction and high detection limit. However, as Okubo et al (66) pointed out, it was essential to optimize the ratio of hydrophobic/hydrophilic monomer in the synthesis to obtain appropriate particle for immunoassay.…”

A novel method to construct a third-generation biosensor: self-assembling gold nanoparticles on thiol-functionalized poly(styrene-co-acrylic acid) nanospheres

“…Recently, there has been considerable interest in the preparation of polymeric particles with diameters of less than a micrometer, not only owing to their unique physical and chemical properties but also wide applications (Clonis, 1987;Okubo et al, 1987). Many techniques are available for the preparation of polymeric spheres.…”