Immuno-serologic studies of the immediate wheal-type allergies

Menzel, Arthur E.O.; Kessler, Walter R.; Cooke, Robert A.; Myers, Phyllis A.

doi:10.1016/0021-8707(52)90035-x

Cited by 14 publications

(8 citation statements)

References 7 publications

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“…In a few instances, however, in the papers referred to in the introduction, reaginic activity was found in fractions which contained no ^/-globulin (5,8). There are two possible explanations.…”

Section: Discussionmentioning

confidence: 99%

“…Some of the workers using the Tisellus cell for the direct separation of "l~-globulins have been able in some experiments to recover only 10 to 25 per cent of the reaginic activity of their sera (3,8). They have inferred from this that the rest of the activity must be in other fractions.…”

Section: Discussionmentioning

confidence: 99%

“…Menzell, Cooke, and associates in 1952 (8), in a preliminary report stated that they had modified their earlier method of separation of allergic sera in the Tiselius cell by using initial chemical fractionation followed by refractionation in the Tiselius cell They found that reaginic activity was associated with the ~/-giobulins; but in some sera there was also apparent activity associated with the a-2 and t-globulins and their interzones. Vaughan, Favour, and Jaffee in 1952 (9), also using chemical fractionation, found reaginic activity mainly in Cohn fraction IIId, although there was some activity in all of their other fractions as well.…”

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confidence: 99%

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The Localization of Skin-Sensitizing Antibody in the Sera of Ragweed-Sensitive Individuals by Electrophoresis

Sehon

Harter

Rose

1956

The Journal of Experimental Medicine

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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The Localization of Skin-Sensitizing Antibody in the Sera of Ragweed-Sensitive Individuals by Electrophoresis

Sehon

Harter

Rose

1956

The Journal of Experimental Medicine

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“…Their resuits suggested that skin-sensitizing activity is concentrated in beta globulin. Menzel and coworkers (22) used combined chemical fractionation and dectrophoresis and found that reagin occurred in the gamma globulin in some, but not all sera, and that beta globulin was present in most specimens which possessed the ability to give passive transfer reactions. Vanghan, Favour, and Jattee (23) fractionated reaginic sera by means of cold ethanol and demonstrated that fraction II (almost pure gamma globulin) was weak in its ability to sensitize human skin as compared with the reactivity of whole serum or of serum fractions containing beta or alpha globulins.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, mixtures of different types of non-precipitating antibody may not be detectable on the basis of a single biological property and it is possible that previous differences in skin activity in fractions from allergic sera separated by electrophoresis (18,22) may have been due in part to the presence of varying amounts of blocking antibody.…”

Section: Discussionmentioning

confidence: 99%

Immunochemical Studies of Antitoxin Produced in Normal and Allergic Individuals Hyperimmunized With Diphtheria Toxoid

Kuhns

1954

Journal of Experimental Medicine

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It has been found that at least two varieties of diphtheria antitoxin are formed in subjects who are hyperimmunized with diphtheria toxoid (1, 2). In a large proportion of individuals the immune response is characterized by the simultaneous production of precipitating and non-precipitating antitoxins. A small proportion of subjects form only one or the other of these antitoxins.Because of its close similarity to allergic reagin of the hay-fever variety, skin-sensitizing antitoxin has been selected as a model for further investigations of the qualities peculiar to reagin. The advantages of the diphtheria system over other less well characterized antigen-antibody systems have been enumerated in an earlier publication (1). In view of studies of the egg white system by Vaughan and Kabat (3) which showed that the immediate type of skin reaction is caused by antibodies to trace substances rather than to the major antigenic component, it was important in our studies to demonstrate that skin sensitization is specifically effected by non-precipitating antitoxin. Evidence has been presented (2, 4) which indicates that immediate wheal reactions are caused by non-precipitating skin-sensitizing antitoxin, and that reactions elicited following the intradermal injection of toxoid are due to specific interaction between toxoid and this variety of antitoxin.In order to gain additional information concerning the nature and specificity of immediate wheal reactions, individual protein components of various sera were isolated with the object of analyzing their comparative skin-sensitizing abilities. It has been demonstrated (5) that the highly purified gamma globulin fraction obtained by cold ethanol precipitation (6) from a serum conraining non-precipitating, skin-sensitizing antitoxin has lost most of its ability to cause immediate wheal reaction although a corresponding antitoxin loss does not occur.

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