Immuno-electron-microscopic study of the prolactin cells in the pituitary gland of male Wistar rats during aging

Putten, L.J.A. van; Kiliaan, Amanda J.

doi:10.1007/bf00215844

Cited by 14 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite no significant change in the percentage of PRL cells in old male rats, old male pituitaries contained more PRL cells than young male ones. This increase in the number of PRL cells seems to correspond closely to the finding by van Putten and Kiliaan (1988) that the PRL-cell volume density significantly increased in male rats at 16-19 months of age. The increase in the number of PRL cells may be partly caused by the enhanced PRL secretion in old rats, since the pituitary cell proliferation is considered to be closely associated with the enhanced release of hormones (Loyd et al, 1975;Billestrup et al, 1986;Takahashi and Kawashima, 1987).…”

Section: Discussionsupporting

confidence: 68%

“…Another important factor which is closely associated with hormone secretions is the pituitary cell population of each secretory cell. Several reports described the age-related changes in the percentages of PRL cells (Takahashi and Kawashima, 1983;Chuknyiska et al, 1986;Sortino and Wise, 1988) and the relative volume of PRL cells (van Putten and Kiliaan, 1988). On the other hand, changes in the GH cell population in the rat are not known.…”

mentioning

confidence: 96%

See 1 more Smart Citation

Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Takahashi¹,

Kawashima

Seo³

et al. 1990

Endocrinol Japon

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 68%

mentioning

confidence: 96%

Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Takahashi¹,

Kawashima

Seo³

et al. 1990

Endocrinol Japon

View full text Add to dashboard Cite

“…However, although exceptional, it is not unique. For instance, in the rat pitui tary gland, Van Putten and Kiliaan (1988) have distin guished four ultrastructurally different cell-types, all of which stain immunocytochemically with antiprolactin. In addition, cells with small round granules, instead of the large polymorphic granules considered to be characteristic 656 for prolactin cells, appear to contain prolactin.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species (trout, flounder and goldfish)

et al. 1989

View full text Add to dashboard Cite

Summary. In order to identify the cell-type responsible for the production of hypocalcin (the recently isolated hypocalcemic hormone of teleost Fish), the corpuscles of Stannius (CS) of trout, flounder and goldfish, were immunocytochemically stained with antisera raised against trout hypo calcin. The secretory granules of the type-1 cells of the CS, considered to be the hypocalcin-producing cells, showed intense immunoreactivity in all species examined. However, in trout and flounder, the secretory granules produced by the type-2 cells, which have been suggested to represent a functionally different cell-type, also showed an intense immunoreactivity. In goldfish, no type-2 cells were ob served. We tentatively conclude that type-1 and type-2 cells represent structurally different forms of the same functional cell-type.

show abstract

“…Neurointermediate lobe tis sue was fixed in Kamovsky's mixture (2% paraform aldehyde and 2% glutaraldehyde in 0.1 M sodium cac odylate, pH 7.4) and embedded in Spurr's resin. Sec tions (90 nm) were collected on 150 mesh nickle grids and immunogold labeled with protein A gold (< J> 16.2 nm; Janssen Biotech NV) essentially as described by Van Putten and Kiliaan (1988). One modification was introduced by omitting etching with H20 2.…”

Section: A T E R Ia L S and M E T H O D Smentioning

confidence: 99%

Morphology of the pars intermedia and the melanophore-stimulating cells in Xenopus laevis in relation to background adaptation

Rijk

Jenks

Bonga

1990

General and Comparative Endocrinology

View full text Add to dashboard Cite

The melanophore-stimulating hormone (MSH) cells of the amphibian pars intermedia secrete the peptide a-melanophore-stimulating hormone (a-MSH), which induces pigment dispersion in dermal melanophores. The purpose of the present study was to determine (1) whether prolonged activation of the secretory activity of the pars intermedia is associated with hypertrophy, hyperplasia, or both and (2) whether the MSH cells function as a homo geneous or heterogeneous population in meeting the physiological demand for MSH. The demand for MSH was manipulated by adapting animals for at least 3 weeks to white, two shades of grey, or black backgrounds. Morphometric analysis showed that the intermediate lobe volume was positively correlated with the degree of pigment dispersion in the mela nophores. The number of MSH cells per lobe was not affected by the degree of pigment dispersion. Therefore, we conclude that enlargement of the tissue associated with MSH cell activation involves hypertrophy rather than hyperplasia. Ultrastructural examination indi cated that the majority of MSH cells in black-adapted animals are biosynthetically active, whereas the cells of white-adapted animals are relatively inactive and show granule storage. The pars intermedia of grey-adapted toads contained both active and inactive cells, indi cating that MSH cells respond as a heterogeneous cell population in meeting the endocrine demand imposed by background. © 1990 Academic Press, inc.In general, activation of endocrine cells may involve hypertrophy, hyperplasia, or both. Furthermore, two patterns of cell re sponses can be distinguished during activa tion: either all cells become activated or only subpopulations of cells become acti vated. An example of the former are the oxytocin-producing neuroendocrine cells that respond as a homogeneous population to the suckling stimulus (Poulain and Wakerley, 1982). An example of the latter are the vasopressin-producing neuroendocrine cells that respond as a heterogeneous pop ulation to osmotic stress (Poulain and Wakerley, 1982). Morphometric and biochemi cal evidence has been advanced that the rat gonadotropes and lactotropes (Lucque et al., 1986) as well as the p cells of the pancreatic islets (Schuit et al., 1988) and thyroid follicular cells in mice (Gerber et al., 1987) form heterogeneous populations with regard to their secretory response to demand for hormone.In the present investigation we studied the response of amphibian melanophorestimulating hormone (MSH)-producing cells to a physiological stimulus. Two major questions were addressed. First, does the activation of the pars intermedia involve hypertrophy, hyperplasia, or both? Second, do the endocrine cells of this tissue function as a homogeneous or as a heterogeneous cell population in response to a demand for MSH? The MSH cell of Xenopus laevis provides a good model to study these ques tions because the pars intermedia activity can be easily manipulated. The MSH cells of animals placed on a black background release a-MSH, which stimulates pigment ...

show abstract

Immuno-electron-microscopic study of the prolactin cells in the pituitary gland of male Wistar rats during aging

Cited by 14 publications

References 20 publications

Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species (trout, flounder and goldfish)

Morphology of the pars intermedia and the melanophore-stimulating cells in Xenopus laevis in relation to background adaptation

Contact Info

Product

Resources

About