Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Takahashi, Sumio; Kawashima, Seiichiro; Seo, Hisao; Matsui, Nobuo

doi:10.1507/endocrj1954.37.827

Cited by 28 publications

(11 citation statements)

References 37 publications

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“…These results are similar to those from studies of mice and rats, which indicate that GH cells are more numerous in males, and FSH cells and PRL cells are more numerous in females. [2][3][4]18,19 However, age-related sex differences for ponies of the study reported here differed from those in rats. In old rats, the percentage of GH and PRL cells was clearly different between sexes, 19 whereas in old horses the percentage of GH cells decreased in both sexes, and the percentage of PRL cells increased only in stallions.…”

Section: Discussioncontrasting

confidence: 73%

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Tan¹,

Sasaki²

2000

ajvr

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Section: Discussioncontrasting

confidence: 73%

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Tan¹,

Sasaki²

2000

ajvr

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“…Pituitary PRL mRNA level has been reported to be either unaltered or decreased with aging (23,39). However, we found that PRL mRNA expression increased 1.8-fold in old AP cells as compared with young ones.…”

Section: Discussioncontrasting

confidence: 50%

Differential Effect of Age on Transforming Growth Factor-β1 Inhibition of Prolactin Gene ExpressionVersusSecretion in Rat Anterior Pituitary Cells¹

Tan¹,

Wang²,

Pu³

et al. 1997

Endocrinology

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Transforming growth factor-beta 1 (TGF-beta 1) synthesized in the pituitary may act as an autocrine/paracrine regulator of lactotrope function. We examined the effects of TGF-beta 1 on PRL messenger RNA (mRNA), PRL synthesis, and PRL secretion in cultured anterior pituitary (AP) cells from rats at different ages. APs excised from ovariectomized female Sprague-Dawley rats, either young(2-3 months old; average serum PRL: 9 ng/ml), middle-aged (11-12 months old; average serum PRL: 133 ng/ml), or old (24 months old; average serum PRL: 159 ng/ml), were dispersed and cultured for 5 days. Then, cells were washed and challenged with increasing doses of TGF-beta 1 (0-100 ng/ml) for 1-48 h in serum-free medium. Northern blot analysis showed an increase in basal PRL mRNA levels, and a decrease in responsiveness to TGF-beta 1 with age. TGF-beta 1 suppressed PRL mRNA in a dose- and time-dependent manner in cells from young rats. Maximum inhibition was observed at 0.5-1 ng/ml of TGF-beta 1. At 0.5 ng/ml TGF-beta 1, significant reduction in PRL mRNA was detected at 6 h, and maximum inhibition was observed at 12-48 h post TGF-beta 1 incubation. Cells from middle-aged rats were less responsive to TGF-beta 1, whereas cells from old rats did not seem to respond under our experimental conditions. In addition to its effect on PRL mRNA in young AP cells, TGF-beta 1 dose dependently inhibited the rate of PRL synthesis, as indicated by reduced [35S]methionine incorporation into immunoprecipitated PRL. Responsiveness of PRL synthesis to TGF-beta 1 inhibition also decreased with age; however, significant inhibition by TGF-beta 1 on PRL synthesis could still be observed in old AP cells. Analysis by RIA demonstrated that young AP cells produced lower levels (15 micrograms/10(6) cells.24 h) of PRL in culture medium than old AP cells (32 micrograms/10(6) cells.24 h). TGF-beta 1 decreased medium PRL levels in old AP cells as efficaciously as in young AP cells. Significant reduction in medium PRL secreted by young AP cells was observed at 3 h when changes in both PRL mRNA and PRL synthesis were not evident. Taken together, our data suggest that TGF-beta 1 affects PRL production at multiple levels. Moreover, its inhibition on PRL synthesis and mRNA expression, but not on PRL secretion, is age-related. Thus, TGF-beta 1 may play an important role in regulating lactotrope function during aging.

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“…It is known that pituitary weight increases with aging in female rats. 4,5) This finding (Table 1) suggests that long-term administration of RJ can attenuate age-associated increases in pituitary weight. Young female rats exhibit estrus once every 4 to 5 d and then go into irregular cycles with aging.…”

mentioning

confidence: 89%

Effects of Long-Term Administration of Royal Jelly on Pituitary Weight and Gene Expression in Middle-Aged Female Rats

Narita

Ohta

Suzuki

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Cited by 28 publications

References 37 publications

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Differential Effect of Age on Transforming Growth Factor-β1 Inhibition of Prolactin Gene ExpressionVersusSecretion in Rat Anterior Pituitary Cells¹

Effects of Long-Term Administration of Royal Jelly on Pituitary Weight and Gene Expression in Middle-Aged Female Rats

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Age-Related Changes in Growth Hormone and Prolactin Messenger RNA Levels in the Rat.

Cited by 28 publications

References 37 publications

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Effect of age on immunocytochemical staining characteristics of adenohypophyseal cells in Mongolian pony mares and stallions

Differential Effect of Age on Transforming Growth Factor-β1 Inhibition of Prolactin Gene ExpressionVersusSecretion in Rat Anterior Pituitary Cells1

Effects of Long-Term Administration of Royal Jelly on Pituitary Weight and Gene Expression in Middle-Aged Female Rats

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Differential Effect of Age on Transforming Growth Factor-β1 Inhibition of Prolactin Gene ExpressionVersusSecretion in Rat Anterior Pituitary Cells¹