Immuno-Electron Microscopic Localization of a Choriogonadotropin-Like Antigen in Cancer-Associated Bacteria

Slifkin, Malcolm; Pardo, Matias; Pouchet-Melvin, G R; Acevedo, Hernan F.

doi:10.1159/000225343

Cited by 11 publications

(6 citation statements)

References 3 publications

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“…This data is in agreement with the findings of Acevedo and his group (1,2,20), who have demonstrated, by immunoelectron microscopy with the indirect peroxidase-antiperoxidase-labeled antibody technique, the presence of the hCG-like material in the membrane of cancerassociated bacteria (both aerobic and anaerobic). Maruo and his colleagues (13) also concluded that the acetone extracts prepared from bacteria isolated from tumors is hCG-like since its elution pattem on a Sephadex G-100 column is similar to that of 125I-labeled hCG.…”

Section: Resultssupporting

confidence: 93%

Tumor-associated bacteria capable of producing a human choriogonadotropin-like substance

Backus¹,

Affronti²

1981

Infect Immun

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Aerobic microorganisms were isolated and identified from 9 of 10 malignant tissues aseptically obtained from surgical patients. The organisms isolated are species commonly associated with the flora of the human body. When these cancer-associated organisms were grown in Trypticase soy broth (BBL Microbiology Systems), a protein substance was isolated from the culture filtrates by acetone precipitation. The acetone precipitates of 12 of 14 organisms tested were positive when assayed by radioimmunoassay for the beta subunit of human chorionic gonadotropin (hCG). All but one of the bacterial isolates from the malignancies were capable of producing the hCG-like substance, but in varying quantities. Control organisms (not isolated from a malignancy) and uninoculated Trypticase soy broth were either completely negative in the radioimmunoassay for beta hCG or had levels of beta hCG near the limit of the sensitivity of the method. These results suggest the possibility that bacteria-tumor relationships do exist and are in agreement with the findings of other workers. Investigation of these relationships may have important and provocative implications in the study of neoplastic diseases. During the past decade a number of investi

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Section: Resultssupporting

confidence: 93%

Tumor-associated bacteria capable of producing a human choriogonadotropin-like substance

Backus¹,

Affronti²

1981

Infect Immun

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“…Finally, the findings of the present studies added to those of our previous investigations (1,2,12,15) continue to support the possibility of the existence of a biological association between these intriguing microorganisms and clinically manifested cancer.…”

Section: Discussionsupporting

confidence: 85%

“…Based on all these results, it appears that the microbes synthesize material similar to both only the complete hormone-like material of S. simulans RU-1 appears to find its way into the culture media. The discrepancy between some of the immunocytochemical findings and the results of the analysis of the extracts may be explained by the demonstration that the bacterial walls were not broken by the cold acetone treatment and may account for the inability to extract the membrane-associated glycoproteins (15). This lack of physical interaction may also explain the negative results obtained in the analyses of the extracts of P. maltophilia ATCC 13637, a cancerassociated bacterial strain that was previously shown to express CG-like material by immunocytochemistry (1).…”

Section: Discussionmentioning

confidence: 93%

“…The presence of choriogonadotropin (CG)-like immunoreactive material in a group of aerobic and anaerobic bacterial strains, originally isolated from tissues of patients having a variety of malignant neoplasms, has been demonstrated by the use of the indirect fluorescein-labeled and the indirect peroxidase-labeled techniques, utilizing antiserum to human CG as well as to its a and ,8 subunits (CGa and CG,f) and fragments (CG,f COOH-terminal peptides) as first antibodies (1,2). Immunoelectron microscopy revealed that the material was associated mainly with the membranes of the cell wall (15). The CG-like antigen was detected neither in other bacteria from similar sources nor in a wide variety of "noncancer" control bacteria that included strains from the American Type Culture Collection (ATCC) as well as laboratory isolates.…”

mentioning

confidence: 99%

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Choriogonadotropin-like antigen in a strain of Streptococcus faecalis and a strain of Staphylococcus simulans: detection, identification, and characterization

Acevedo¹,

Koide²,

Slifkin³

et al. 1981

Infect Immun

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The presence of choriogonadotropinand a-subunit-like materials in two species of bacteria identified as Staphylococcus simulans and Streptococcus faecalis have been demonstrated by the indirect fluorescein-labeled and the indirect peroxidase-labeled immunocytochemical techniques, utilizing antiserum for human choriogonadotropin, for its a and ,8-subunits and the fl-subunit COOHterminal peptide. The bacteria were originally isolated from the urine of two patients with advanced forms of cancer. Chromatography done on the watersoluble extract of acetone powder preparations of the bacterial cultures revealed the presence of a material similar to the complete trophoblastic hormone and to its fl-subunit in the culture media of S. simulans, and to the fl-subunit in the media of S. faecalis. No free a-subunit was detectable. Furthermore, the choriogonadotropin-like factor demonstrated biological activity in in vivo assay systems. From the present results, it can be concluded that some species of "cancerassociated" bacteria can synthesize a human trophoblastic hormone-like glycoprotein with physicochemical properties similar to those of the human trophoblastic hormone that is biologically active and that is either released complete or as one of its subunits in the culture media. 487 on July 31, 2020 by guest http://iai.asm.org/ Downloaded from

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“…Unicellular organisms like bacteria and yeasts have been identified to acquire genes of certain hormones. Of fundamental importance is the fact that some bacteria isolated from cancer patients expressed hCG, but not every isolated bacteria did (28–33). Moreover, the morphologic changes observed by electron microscopy (34) indicated that some of the bacteria expressing the hCG‐like material are revertants of cell wall deficient (CWD) variants.…”

Section: Introductionmentioning

confidence: 99%

Human chorionic gonadotropin (hCG), the hormone of life and death: a review

Acevedo

2002

J Exp Therapeutics

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Immuno-Electron Microscopic Localization of a Choriogonadotropin-Like Antigen in Cancer-Associated Bacteria

Cited by 11 publications

References 3 publications

Tumor-associated bacteria capable of producing a human choriogonadotropin-like substance

Tumor-associated bacteria capable of producing a human choriogonadotropin-like substance

Choriogonadotropin-like antigen in a strain of Streptococcus faecalis and a strain of Staphylococcus simulans: detection, identification, and characterization

Human chorionic gonadotropin (hCG), the hormone of life and death: a review

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