Immuno-capture of cells in open microfluidics: microfluidic probes integrated with herringbone micro-mixers

Glia, Ayoub; Sukumar, Pavithra; Brimmo, Ayoola T.; Deliorman, Muhammedin; Qasaimeh, Mohammad A.

doi:10.1117/12.2516035

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…The instantaneous speed and height was deduced by the size of the major and minor axis of each bead [38,39]. Because the imaging system was focused onto the bottom surface of the cell, we assume that the size of the minor axis corresponded to the height of the beads due to the defocused PSF.…”

Section: Resultsmentioning

confidence: 99%

Tunable SIM: observation at varying spatiotemporal resolutions across the FOV

Woo

Jung

Ahn

et al. 2020

Optica

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To date, imaging systems have generally been designed to provide an even spatiotemporal resolution across the field of view (FOV). However, this becomes a fundamental limitation when we aim to simultaneously observe varying dynamics at different parts of the FOV. In conventional imaging systems, to capture fast dynamics occurring at only a small portion of the FOV, the entire imaging system's sampling rate must be increased. This is a major problem if different parts of the FOV must rather be imaged at high spatial resolutions beyond the diffraction limit and require a sacrifice in temporal resolution. To answer this unmet challenge, we propose tunable SIM, which enables adaptive modulation of spatiotemporally varying structured illumination across different parts of the FOV. Using tunable SIM, we exploit the varying and designable spatiotemporal resolution to demonstrate simultaneous measurements of subdiffraction-limited changes in the actin fine structure of U87MG-EGFP-CD9 cells and the fast viscous flow inducing these structural changes.

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Section: Resultsmentioning

confidence: 99%

Tunable SIM: observation at varying spatiotemporal resolutions across the FOV

Woo

Jung

Ahn

et al. 2020

Optica

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Section: Introductionmentioning

confidence: 99%

“…[36] The flow behavior of Newtonian fluids in slanted groove and staggered herringbone micromixers has been extensively addressed, showing that the anisotropy of groove patterns allows to engineer flow close to walls. [37][38][39][40] However, the flow of multiphase yield stress fluids in a staggered herringbone roughness has not been considered. In particular, despite extensive evidence that nonlocal fluidization is triggered by surface roughness, [17][18][19][41][42][43][44] the role of directional roughness has not been addressed so far.…”

Section: Introductionmentioning

confidence: 99%

Boost and Contraction of Flow by Herringbone Surface Design on the Microfluidic Channel Wall

Filippi¹,

Derzsi

Nalin

et al. 2023

Adv Materials Technologies

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The handling of yield-stress fluids typically involves a jammed-to-flow transition that is pivotal for many injection and transport technologies on different scales, such as additive manufacturing, injection molding, food rheology, and oil transport. For all of these applications, it is crucial to be able to tune the fluidization under constant load. The pressure-driven flow of emulsions is reported within a microfluidic channel, one wall of which is patterned by a herringbone-riblet roughness comprising a regular array of V-shaped grooves. With respect to the pressure gradient, this pattern displays a convergent (divergent) orientation that provides a forward (backward) direction. At the tip of the herringbone pattern, the forward and backward flows are almost identical for a viscous Newtonian fluid and a diluted emulsion, whereas a surprising flow boost in the forward direction is observed as the emulsion approaches a jammed state. The flow boost is more effective at small herringbone angles and low pressure loads. 3D velocity profiles along the channel cross-section unravel unexpected heterogeneous stress, band-like distributed, with evidence of nonlocal correlations.

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Stainless microfluidic probe with 2D-array microapertures

et al. 2021

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A microfluidic probe (MFP) does not require physical walls for flow channels, enabling application of a chemical solution in an open space by injecting and aspirating the solution. However, in conventional MFP fabrication methods, the use of a 2D aperture array at narrow intervals to enhance the function of treatment remains limited. In this study, we developed a stainless MFP (stMFP) to produce a 2D aperture array at narrow intervals. The stMFP was developed using a stacking technique in which stainless steel substrates fabricated by photolithography and a wet etching process were stacked and bonded through thermal diffusion. This process resulted in a 6-row and 8-column aperture array with an aperture size of 100 × 150 µm and a narrow interval of 50 µm. The surface treatment area was evaluated by biopatterning of a fluorescent antibody. The results showed that the stMFP biopatterned a minimum treatment area of 3.3 × 103 µm2, which could be controlled between 5.1 × 104 µm2 and 3.0 × 105 µm2 by changing the aperture arrangement. In addition, when two types of fluorescent antibodies were alternately injected in the row direction, six independent treatment areas of 6.1 × 104 µm2 were formed over a wide area of 3.8 × 105 µm2. Furthermore, biopatterning using a 4 × 4 aperture array showed that a 2D treatment area with 4-rows and 2-columns can be produced with an area of 4.5 × 105 µm2. A single stMFP can form various 2D treatment patterns, which is expected to realize high-performance bioprocessing in the field of biology.

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Immuno-capture of cells in open microfluidics: microfluidic probes integrated with herringbone micro-mixers

Cited by 4 publications

References 12 publications

Tunable SIM: observation at varying spatiotemporal resolutions across the FOV

Tunable SIM: observation at varying spatiotemporal resolutions across the FOV

Boost and Contraction of Flow by Herringbone Surface Design on the Microfluidic Channel Wall

Stainless microfluidic probe with 2D-array microapertures

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