Immunization with the binding domain of FimH, the adhesin of type 1 fimbriae, does not protect chickens against avian pathogenicEscherichia coli

Abstract

(2005) Immunization with the binding domain of FimH, the adhesin of type 1 fimbriae, does not protect chickens against avian pathogenic Escherichiacoli , Avian Pathology, 34:3, 264-272,

Immunization with the biologically active lectin domain of PapGII induces strong adhesion-inhibiting antibody responses but not protection against avian pathogenicEscherichia coli

“…APEC strain CH2 was a virulent O78 papG II ' strain (Van den Bosch et al , 1993;Vandemaele et al , 2005). APEC 1 was a virulent O45 papG II ' strain (Vandemaele et al , 2003b).…”

“…Infections with NDV lentogenic strains Lasota and Clone30 (Intervet International) were used to predispose chickens to colibacillosis, as described by Vandemaele et al (2005). Each chick in Experiment 1 was administered approximately 2 )/10 6.3 median egg infective doses of strain Lasota in phosphate-buffered saline (PBS) by spray.…”

“…In a previous study (Vandemaele et al , 2005), we demonstrated that the sugar binding domain of FimH, the adhesin of F1A fimbriae, did not protect chickens against APEC infection, indicating a more limited role in infection. However, a major difficulty encountered was the induction of a mucosal immune response in the trachea with a single protein (Vandemaele et al , 2005).…”

“…However, a major difficulty encountered was the induction of a mucosal immune response in the trachea with a single protein (Vandemaele et al , 2005). Due to the suggested role of PapG in the systemic phase of APEC infection (Dozois et al , 1995;Pourbakhsh et al , 1997a), the aim of this study was to investigate whether systemic antibodies against the biologically active recombinant PapGII sugar binding domain (i.e.…”

“…Fimbriae are proteinaceous structures on the outer membrane of E. coli , mediating attachment to host cell receptors by means of their conserved adhesin. F1A or type 1 fimbriae are believed to be involved in the initial colonization of the respiratory tract due to adhesion of the adhesin FimH to D-mannose residues on host cells (Gross, 1994;Pourbakhsh et al , 1997a,b), although their exact role remains much debated (Marc et al , 1998;Arné et al , 2000;Vandemaele et al , 2005). P fimbriae, on the other hand, are thought to act in the later stages of infection, as they are only expressed in vivo in the lower respiratory tract and internal organs (Dozois et al , 1995;Pourbakhsh et al , 1997a).…”