Immunization procedures for anti-idiotypic antibody induction in mice and rats

Maruyama, Haruhiko; Sperlagh, Melinda; Žaloudík, Jan; Liang, Shaohong; Mizuki, Kiyoshi; Molthoff, Carla F. M.; Herlyn, Dorothee

doi:10.1016/s0022-1759(02)00091-1

Cited by 9 publications

(6 citation statements)

References 31 publications

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“…These include the use of anti-idiotypic antibodies, anti-immunocomplex (anti-IC) antibodies, − and recombinant VH (variable heavy domain of antibody) and VL (variable light domain of antibody) to make an open sandwich immunoassay. , Among these attempts, the use of anti-IC binders closely resembles the two-site immunometric assay. However, development of anti-IC antibodies for small molecules by traditional animal immunization is a tedious and problematic procedure with low success rate . Recently, a phage display-based strategy for more controlled generation of anti-IC binders from a naïve antibody library was described to establish noncompetitive assays for two haptens: morphine and (−)-Δ9-tetrahydrocannabinol .…”

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confidence: 99%

“…However, development of anti-IC antibodies for small molecules by traditional animal immunization is a tedious and problematic procedure with low success rate. 12 Recently, a phage displaybased 13 strategy for more controlled generation of anti-IC binders from a nai ̈ve antibody library was described to establish noncompetitive assays for two haptens: morphine 14 and (−)-Δ9-tetrahydrocannabinol. 15 A related approach involved the isolation of specific immunocomplex (IC) binding peptides from a phage peptide library and the use of whole peptide displaying phage for assay development.…”

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confidence: 99%

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Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

et al. 2016

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A broad-spectrum noncompetitive immunoassay allowing sensitive and simple detection of a group of similar compounds would be an ideal tool for screening low-molecular weight analytes (<2000 Da) having many variants. However, the development of an essential antibody pair capable of sandwich-type recognition of the analytes' small generic core structure is a demanding task due to limited space available for simultaneous binding of two different antibodies. We report here a generic noncompetitive assay for cyanobacterial microcystins (MCs) and nodularins (Nod), a group of structurally related small cyclic peptides (∼1000 Da) with more than 100 naturally occurring analogs. The assay is based on the unique combination of a generic anti-immunocomplex (anti-IC) single-chain fragment of antibody variable domain (scFv) and a monoclonal antibody capable of binding to an Adda-group (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4(E),6(E)-dienoic acid) present in all MCs/Nod. The anti-IC scFv was isolated from a large synthetic antibody library with phage display and used to develop a single-step sandwich-type noncompetitive immunocomplex assay. The sensitive time-resolved immunofluorometry-based assay is capable of detecting all the 11 tested commonly occurring hepatotoxins (MC-LR, -dmlR, -RR, -dmRR, -LA, -LY, -LF, -LW, -YR, -WR, and Nod-R) at concentration below 0.1 μg/L in a 1 h assay. Using MC-LR, the most studied toxic and widely distributed of the toxins, the calculated detection limits (based on blank + 3SD response) are ∼0.026 μg/L in 1 h and ∼0.1 μg/L in 10 min assay time. This is by far the fastest reported immunoassay for MCs and Nod with a detection limit far below the World Health Organization's guideline limit (1 μg/L of MC-LR equivalent in drinking water). The assay was validated with spiked tap and lake water as well as with environmental surface water samples. The developed assay provides a simple, rapid, and highly sensitive tool for the quantitative detection of MCs/Nod with the additional benefit of automation and high-throughput possibilities for large scale screening of drinking and environmental surface water samples. Furthermore, the study describes the first demonstration of the assay intended for the detection of an analyte group comprising similar low-molecular weight compounds exhibiting the benefits of a reagent excess type assay.

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confidence: 99%

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Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

et al. 2016

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“…Complex of a class of Ig with another class of Ig has been thought an immune regulation factor because it may result from the combination of Ab1 with Ab2 in the immune network [14][15][16]. There are several types of the complexes, some of which were detected in warm autoimmune haemolytic anaemia, gastric cancer, hepatitis A and Graves' diseases and showed their importance in immune regulation and clinical significance [2][3][4]10].…”

Section: Discussionmentioning

confidence: 99%

Detection of IgM and IgG complexes provides new insight into immune regulation of patients with malignancies: A randomized controlled trial

Yang

Huang

et al. 2007

International Immunopharmacology

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“…One of the main limitations for more widespread use of anti-immunocomplex specific antibodies is the tedious antibody development process, which can be very challenging with traditional methods based on the immunization of animals [13]. With the introduction of in vitro antibody development methods, the selection conditions throughout the antibody development process can be controlled more efficiently enabling the generation of antibodies with predefined specificity to complex targets.…”

Section: Introductionmentioning

confidence: 99%

Development of anti-immunocomplex specific antibodies and non-competitive time-resolved fluorescence immunoassay for the detection of estradiol

et al. 2019

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Detection of circulatory estradiol has widespread use in various clinical applications. Particularly, the use of estradiol-specific antibodies in immunoassays is routinely used, mainly due to the cost efficiency and simplicity of the sample handling process. However, the circulatory levels of estradiol can be extremely low in some conditions, and beyond the current detection limit of existing competitive immunoassays. We describe the generation of anti-immunocomplex specific antibodies derived from synthetic antibody repertoire and the development of high-performance non-competitive immunoassay for the detection of estradiol. Phage display selections were used to isolate new antibodies from synthetic antibody library with the use of existing estradiol specific Fab fragment. The found antibodies were consecutively used to set up a time-resolved fluorescence-based immunoassay (TRFIA), which can be used to detect estradiol with exceptional sensitivity and specificity. The limit of detection and EC50 were shown to be 3.0 pg mL −1 and 32.4 pg mL −1 respectively. Graphical abstract

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Immunization procedures for anti-idiotypic antibody induction in mice and rats

Cited by 9 publications

References 31 publications

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

Broad-Spectrum Noncompetitive Immunocomplex Immunoassay for Cyanobacterial Peptide Hepatotoxins (Microcystins and Nodularins)

Detection of IgM and IgG complexes provides new insight into immune regulation of patients with malignancies: A randomized controlled trial

Development of anti-immunocomplex specific antibodies and non-competitive time-resolved fluorescence immunoassay for the detection of estradiol

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