Immunization of chickens with Salmonella enterica subspecies enterica serovar Enteritidis pathogenicity island-2 proteins

Wisner, Amanda L. S.; Desin, Taseen S.; Lam, Po-King S.; Berberov, Emil M.; Mickael, Claudia; Townsend, H. G. G.; Potter, Andrew; Köster, Wolfgang

doi:10.1016/j.vetmic.2011.05.041

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…These data indicate that immunization with the S. Enteritidis ghost via the oral route is effective. In a previous report, immunization of chickens with S. Enteritidis pathogenic island 2 proteins as the subunit vaccine protected organs against infection but did not protect against cecal colonization after challenge with a virulent S. Enteritidis strain (42). Based on our protection assay results, vaccination with the S. Enteritidis ghost prevented or reduced the bacterial counts in internal organs, which further decreased the chance for poultry food product contamination.…”

Section: Cd4supporting

confidence: 53%

“…Because the frequency of antimicrobial resistance and the number of resistance determinants in Salmonella have risen markedly (13), vaccination plays an important role in the overall biosecurity system on chicken farms to prevent Salmonella infections. Several formulations of live attenuated, killed, and subunit vaccines have been used in an attempt to control S. Enteritidis (29,33,42). The commercial vaccines available against S. Enteritidis are mostly inactivated formulations; however, these strategies can affect the physiochemical/structural properties of surface antigens and thereby negatively affect the development of protective immunity.…”

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confidence: 99%

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Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

et al. 2012

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ABSTRACTA new strategy to develop an effective vaccine is essential to control food-borneSalmonella entericaserovar Enteritidis infections. Bacterial ghosts (BGs), which are nonliving, Gram-negative bacterial cell envelopes, are generated by expulsion of the cytoplasmic contents from bacterial cells through controlled expression using the modified cI857/λ PR/geneEexpression system. In the present study, the pJHL99 lysis plasmid carrying the mutated lambda pR37-cI857 repressor and PhiX174 lysis geneEwas constructed and transformed inS. Enteritidis to produce a BG. Temperature induction of the lysis gene cassette at 42°C revealed quantitative killing ofS. Enteritidis. TheS. Enteritidis ghost was characterized using scanning and transmission electron microscopy to visualize the transmembrane tunnel structure and loss of cytoplasmic materials, respectively. The efficacy of the BG as a vaccine candidate was evaluated in a chicken model using 60 10-day-old chickens, which were divided into four groups (n= 15), A, B, C, and D. Group A was designated as the nonimmunized control group, whereas the birds in groups B, C, and D were immunized via the intramuscular, subcutaneous, and oral routes, respectively. The chickens from all immunized groups showed significant increases in plasma IgG and intestinal secretory IgA levels. The lymphocyte proliferation response and CD3+CD4+and CD3+CD8+T cell subpopulations were also significantly increased in all immunized groups. The data indicate that both humoral and cell-mediated immune responses are robustly stimulated. Based on an examination of the protection efficacy measured by observations of gross lesions in the organs and bacterial recovery, the candidate vaccine can provide efficient protection against virulent challenge.

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Section: Cd4supporting

confidence: 53%

mentioning

confidence: 99%

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

et al. 2012

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“…Serum IgY was also increased after both vaccination doses (Table 1(b)), indicating that, although there was a decrease in the percentage of circulating B-lymphocytes, this probably was followed by an enrichment of this cell population in the spleen-which is necessary for strong antibody production-favoring the theory that the cells from the blood were directed to lymphoid sites. The present study confirms results from the literature for IgY production after bacterin immunization, which was correlated elsewhere to lower Salmonella colonization of organs [11].…”

Section: Innactivated Vaccinesupporting

confidence: 92%

“…Both the live and inactivated vaccine types discussed in this work have been shown to be effective in inducing protection and inreducing organ colonization after challenge. However, previous studies have not yet explored the immune mechanisms through which these vaccines exert their effects [8]- [11]. Indeed, the immune mechanisms involved in the protection against Salmonella in poultryare not well known [12] [13].…”

Section: Introductionmentioning

confidence: 99%

Live and Inactivated <i>Salmonella</i> Enteritidis Vaccines: Immune Mechanisms in Broiler Breeders

Bérto¹,

Beirão²,

Filho³

et al. 2015

WJV

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Salmonella is a ubiquitous pathogen which, in addition to causing poultry diseases, has a growing zoonotic impact. It has demanded the implementation of diverse control strategies, in which vaccines play a major role. The understanding of the immune pathways elicited by the different vaccines is important, contributing for the establishment of strong immune correlates of protection, for instance. With the purpose of determining the dynamics of the humoral and cellular immune responses to vaccination, broiler breeders (Cobb Slow) were immunized with live or inactivated vaccines against Salmonella Enteritidis. Lymphocyte and macrophage subsets were analyzed in the peripheral blood by flow cytometry and antigen-specific circulating IgY and mucosal IgA were quantified. The markers analyzed by flow cytometry were CD8/CD28, CD4/TCRVβ1, Kul/ MHC II and Bu-1. Both live and inactivated vaccines induced an increase in the proportion of circulating monocytes (Kul + MHCII + ) in some time points compared to non-vaccinated controls. However, whereas the live vaccine leads to an increase in CD8 − CD28 + and Bu-1 + lymphocytescompared to the control group, the inactivated vaccine prompteda reduction in the percentage of severalleucocyte subsets (Kul − MHCII + , Bu-1 + , CD8 + CD28 + , CD8 − CD28 + , CD4 + TCRVβ1 − , CD4 + TCRVβ1 + , CD4 − TCRVβ1 + ) after the boost dose. Both vaccines induced specific serum IgY and mucosal IgA production; however, the inactivated vaccine stimulated higher titers in a shorter period. These results contribute to the understanding of mechanisms of action of live and inactivated Salmonella vaccines in chickens.

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“…However, in this vaccine trial, the authors administered the bacterial challenge via the intravenous route, making the comparison with other similar studies difficult. In a more recent study, immunization of chickens with SPI-1 and SPI-2 proteins demonstrated a mild reduction of the challenge strain in the internal organs while the cecal colonization was unaffected Wisner et al, 2011). This suggests that a combination of Salmonella T3SS and flagellar proteins (and possibly other candidate proteins) may form a highly effective vaccine since live vaccine strains that contain deletions in T3SS genes as well as flagellar genes have shown to be promising.…”

Section: Nontyphoidal Salmonella Speciesmentioning

confidence: 99%

Potential Impact of Food Safety Vaccines on Health Care Costs

GhunaimHaitham

DesinTaseen

2015

Foodborne Pathogens and Disease

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Foodborne pathogens continue to cause several outbreaks every year in many parts of the world. Among the bacterial pathogens involved, Shiga toxin-producing Escherichia coli, Campylobacter jejuni, and nontyphoidal Salmonella species cause a significant number of human infections worldwide, resulting in a huge annual economic burden that amounts to millions of dollars in health care costs. Human infections are primarily caused by the consumption of contaminated food. Vaccination of food-producing animals is an attractive, cost-effective strategy to lower the levels of these pathogens that will ultimately result in a safer food supply and fewer human infections. However, producers are often reluctant to routinely vaccinate animals against these pathogens since they do not cause any detectable clinical symptoms. This review highlights recent approaches used to develop effective food safety vaccines and the potential impact these vaccines might have on health care costs.

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Immunization of chickens with Salmonella enterica subspecies enterica serovar Enteritidis pathogenicity island-2 proteins

Cited by 14 publications

References 26 publications

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

Live and Inactivated <i>Salmonella</i> Enteritidis Vaccines: Immune Mechanisms in Broiler Breeders

Potential Impact of Food Safety Vaccines on Health Care Costs

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Immunization of chickens with Salmonella enterica subspecies enterica serovar Enteritidis pathogenicity island-2 proteins

Cited by 14 publications

References 26 publications

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ PR/GeneEExpression System

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ PR/GeneEExpression System

Live and Inactivated &lt;i&gt;Salmonella&lt;/i&gt; Enteritidis Vaccines: Immune Mechanisms in Broiler Breeders

Potential Impact of Food Safety Vaccines on Health Care Costs

Contact Info

Product

Resources

About

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

Characterization of a Novel Inactivated Salmonella enterica Serovar Enteritidis Vaccine Candidate Generated Using a Modified cI857/λ P_R/GeneEExpression System

Live and Inactivated <i>Salmonella</i> Enteritidis Vaccines: Immune Mechanisms in Broiler Breeders