Immunity and protection against

Golding, Basil; Scott, Dorothy E.; Scharf, Orit; Huang, Liyun; Zaitseva, Marina; Lapham, Cheryl; Eller, Nancy; Golding, Hana

doi:10.1016/s1286-4579(00)01350-2

Cited by 186 publications

(148 citation statements)

References 38 publications

Supporting

Mentioning

137

Contrasting

Unclassified

Order By: Relevance

“…Even though the Th1-type of immune response is BRUCELLA-GROEL DNA VACCINE 25 thought to be responsible for resistance against facultatively intracellular bacterial pathogens such as B. abortus, i.m. GroEL DNA immunisation was not able to confer protective immunity on vaccinated mice [32]. Recently, Turner et al reported a total lack of protection in mice immunised with Mycobacterium GroEL gene when challenged [33].…”

Section: Discussionmentioning

confidence: 99%

Induction of a Th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene

Leclerq¹,

Harms²,

Rosinha³

et al. 2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

The immunogenicity and protective ef®cacy of a DNA vaccine encoding the GroEL heatshock gene from Brucella abortus was tested in BALB/c mice immunised by intramuscular (i.m.) needle injection or epidermally by gene gun. The Brucella GroEL gene was ampli®ed by PCR and cloned into two different mammalian expression vectors pCMV-link and pCMV-tPA. The D17 cell line was transfected with both constructs and GroEL transcripts were detected by Northern blot. To determine the level of protein synthesised, transfected cell lysates were then submitted to Western blot. The nonsecreted form of the recombinant GroEL produced by the pCMV-link construct was detected in much greater amount than the secreted form of the protein produced by the pCMV-tPA construct. After immunisation, a strong anti-GroEL IgG response was detected in mice vaccinated by i.m. injection or gene gun only when the pCMV-link/ GroEL plasmid was used. Regarding the pattern of immune response induced, i.m. needle injection raised a predominantly Th1 response with mostly IgG2a-speci®c antiGroEL and high levels of IFN-ã produced by splenic T cells. Gene gun immunisation induced a Th0 type of immune response in mice characterised by a high IgG1/IgG2a ratio, and IL-4 and interferon (IFN)-ã production. Even though a distinct pattern of immune response was generated depending upon the immunisation route used, neither method engendered a signi®cant level of protection with the GroEL DNA vaccine.

show abstract

Section: Discussionmentioning

confidence: 99%

Induction of a Th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene

Leclerq¹,

Harms²,

Rosinha³

et al. 2002

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…A single-cell suspension was prepared from the marrow plugs by repeated gentle passage through a needle. Cells were then centrifuged for 5 min at 600 g, and the pellet was resuspended in Red Blood Cell Lysis Buffer (155 mM NH 4 Cl, 10 mM KHCO 3 , 0.1 mM EDTA, pH 7.4, sterilized through a 0.22-mm filter) for 5 min at 37 uC to lyse the erythrocytes. The cells were washed twice in medium and resuspended in RPMI 1640 containing 20% supernatant from L929 cells, 10% FBS, 100 U/ml penicillin and 100 mg/ml streptomycin and incubated at 37 uC in 5% CO 2 .…”

Section: Rt-pcr and Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

“…2 Infection with B. abortus potently activates both the innate and adaptive parts of the immune system, leading to a proinflammatory response that favors the T-helper 1 (Th1) responses. 3,4 Although both antibody (Ab)-and cell-mediated immune responses can influence the course of infection with Brucella, the latter are primary for clearance of intracellular bacteria.…”

Section: Introductionmentioning

confidence: 99%

TLR2 and TLR4 signaling pathways are required for recombinant Brucella abortus BCSP31-induced cytokine production, functional upregulation of mouse macrophages, and the Th1 immune response in vivo and in vitro

Yuan

Gao

et al. 2014

Cell Mol Immunol

View full text Add to dashboard Cite

Brucella abortus is a zoonotic Gram-negative pathogen that causes brucelosis in ruminants and humans. Toll-like receptors (TLRs) recognize Brucella abortus and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. In this study, we focused on recombinant Brucella cell-surface protein 31 (rBCSP31) to determine its effects on mouse macrophages. Our results demonstrated that rBCSP31 induced TNF-a, IL-6 and IL-12p40 production, which depended on the activation of mitogen-activated protein kinases (MAPKs) by stimulating the rapid phosphorylation of p38 and JNK and the activation of transcription factor NF-kB in macrophages. In addition, continuous exposure (.24 h) of RAW264.7 cells to rBCSP31 significantly enhanced IFN-c-induced expression of MHC-II and the ability to present rBCSP31 peptide to CD4 1 T cells. Furthermore, we found that rBCSP31 could interact with both TLR2 and TLR4. The rBCSP31-induced cytokine production by macrophages from TLR2 2/2 and TLR4 2/2 mice was lower than that from C57BL/6 macrophages, and the activation of NF-kB and MAPKs was attenuated in macrophages from TLR2 2/2 and TLR4 2/2 mice. In addition, CD4 1 T cells from C57BL/6 mice immunized with rBCSP31 produced higher levels of IFN-c and IL-2 compared with CD4 1 T cells from TLR2 2/2 and TLR4 2/2 mice. Macrophages from immunized C57BL/6 mice produced higher levels of IL-12p40 than those from TLR2 2/2 and TLR4 2/2 mice. Furthermore, immunization with rBCSP31 provided better protection in C57BL/6 mice than in TLR2 2/2 and TLR4 2/2 mice after B. abortus 2308 challenge. These results indicate that rBCSP31 is a TLR2 and TLR4 agonist that induces cytokine production, upregulates macrophage function and induces the Th1 immune response.

show abstract

“…Induction of a strong IL-2 response along with relative predominance of IgG2a antibodies in the mice immunised with CpG'rOMP28 suggested that the addition of CpG ODN elicited Th1 type of immune response (Cassataro et al 2004). Moreover, the importance of IL-2 in control of intracellular pathogens including Brucella has been supported by earlier studies (Golding et al 2001). An interesting finding of the present study was that the mice immunised with rOMP28 alone had significantly lower (P !0.05) production of IL-2 (Figure 2).…”

Section: Resultsmentioning

confidence: 74%

Effect of CpG oligodeoxynucleotide on the immune response of 28 kDa outer membrane protein ofBrucella

Kaushik

Singh

Dayal

et al. 2011

Journal of Applied Animal Research

View full text Add to dashboard Cite

In order to develop an effective new generation vaccine against brucellosis, it is important to identify and evaluate the immune response of protein antigens of Brucella. It has been demonstrated that 28 kDa outer membrane protein (OMP28) of Brucella induced Th2 type of immune response with predominant IgG1 and suppressed IL-2 production. However, coadministration of CpG oligodeoxynucleotide caused a shift in the immune response of antigen towards Th1 type with predominant IgG2a and upregulated IL-2 production. This suggested that the OMP28 of Brucella may act as a B-cell antigen and may induce Th1 type of immune response on addition of suitable adjuvant.

show abstract

Immunity and protection against

Cited by 186 publications

References 38 publications

Induction of a Th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene

Induction of a Th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene

TLR2 and TLR4 signaling pathways are required for recombinant Brucella abortus BCSP31-induced cytokine production, functional upregulation of mouse macrophages, and the Th1 immune response in vivo and in vitro

Effect of CpG oligodeoxynucleotide on the immune response of 28 kDa outer membrane protein ofBrucella

Contact Info

Product

Resources

About