Effect of CpG oligodeoxynucleotide on the immune response of 28 kDa outer membrane protein ofBrucella

Abstract

In order to develop an effective new generation vaccine against brucellosis, it is important to identify and evaluate the immune response of protein antigens of Brucella. It has been demonstrated that 28 kDa outer membrane protein (OMP28) of Brucella induced Th2 type of immune response with predominant IgG1 and suppressed IL-2 production. However, coadministration of CpG oligodeoxynucleotide caused a shift in the immune response of antigen towards Th1 type with predominant IgG2a and upregulated IL-2 production. This suggested that the OMP28 of Brucella may act as a B-cell antigen and may induce Th1 type of immune response on addition of suitable adjuvant.