Immunisation against East Coast fever by the infection and treatment method: evaluation of the use of ice baths for field delivery and appraisal of an acid formulation of long-acting tetracycline

Marcotty, Tanguy; Billiouw, M.; Chaka, G.; Berkvens, Dirk; Losson, Bertrand; Brandt, J.

doi:10.1016/s0304-4017(01)00462-9

Cited by 13 publications

(17 citation statements)

References 12 publications

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“…In several African countries, control of ECF relies on the so-called infection and treatment technique. In this approach, animals are actively infected with T. parva sporozoïtes and treated at the same time with long acting oxytetracycline (Marcotty et al 2001;Kivaria et al 2007). At the present time, this approach seems difficult to implement in North Kivu province and more generally in DRC.…”

Section: Discussionmentioning

confidence: 99%

Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo

et al. 2012

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“…Before ASVEZA, another Belgium sponsored animal disease control project (BADCP), played a vital role in immunization against Theileriosis. Significant levels of donor support for the control of tickborne diseases have also been and continue to date to be received through the European Union (EU) funded Southern Africa Animal Disease Control Program (SAADCP), the Private and Co-operative Livestock Services Network Development Program (PCLSNDP) and the United [44]. However, there are problems associated with each control approach.…”

Section: Controlmentioning

confidence: 99%

The current status of major tick borne diseases in Zambia

Makala

Mangani²,

Fujisaki

et al. 2003

Vet. Res.

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-Tick-borne diseases occurring in Zambia are assuming more importance as they continue to be a major economic problem not only in Zambia, but in many parts of Eastern, Southern and Central Africa. The current control methods, which include the use of toxic acaricides to kill ticks, and the virulent sporozoite infection and treatment method have limitations. Recombinant vaccines, currently in their experimental stages, offer hope for the future. The use of acaricides is hampered by the development of acaricide resistance and live vaccines are dependent on cold chain facilities, which are a formidable obstacle in the poorly developed infrastructure in parts of Zambia where the vaccine is most needed. Amidst these drawbacks are the results of the recent research on parasites and vector recombinant vaccines which promise to circumvent these problems. The history, current status and attitudes regarding the control of these diseases, taking into account their complexity, are reviewed. The establishment of the well-designed Central Veterinary Research Institute (CVRI) and Japanese International Cooperation Agency (JICA) sponsored veterinary school, both have a potential for high quality research, with access to a wealth of specimens a veritable goldmine of research material. It is thus hoped that this review will stimulate the desire to maximize the value of the tick and tick-borne disease research in both Zambia and the international research community.

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“…Katete stabilates were all produced from batches of adult R. appendiculatus ticks, infected as nymphs with the same T. parva seed stabilate. The first glycerated Katete stabilate (K1g) that was used in this study had been produced and tested in vivo by Marcotty et al (2001). The two other Katete stabilates, K2g and K2s, were produced for the present work from a single batch of infected ticks but were cryoprotected using 7.5 % (w/v) glycerol and 0.3 M (10 % w/v) sucrose, respectively.…”

Section: Stabilates Usedmentioning

confidence: 99%

“…This method allows the distribution of the service using bicycles instead of motor cars. Studies conducted in this region using a glycerated T. parva Katete stock stabilate (Geysen, Bishop, Skil ton, Dolan & Morzaria 1999) showed that after 6 h of storage on ice stabilates could still protect 90 % of the immunized animals (Marcotty et al 2001). In the southern province of Zambia, the I&T immunization against ECF makes use of the milder Chitongo strain (Geysen et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Comparison of the survival on ice of thawed <i>Theileria parva</i> sporozoites of different stocks cryoprotected by glycerol or sucrose

Mwanakasale¹,

Berkvens²,

Dorny³

et al. 2007

Onderstepoort J Vet Res

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Stabilates of Theileria parva sporozoites are mostly delivered in liquid nitrogen tanks to the East Coast fever immunization points. Using an in vitro titration model, we assessed the loss of infectivity of several stabilates when they are stored in ice baths for up to 24 h. Comparisons, with respect to rates of loss of infectivity, were made between T. parva stocks (Chitongo and Katete), cryoprotectants (sucrose and glycerol) and method of assessment (in vivo and in vitro techniques). Chitongo and Katete stabilates showed similar loss dynamics. The losses were 1-4 % (depending on parasite stock) and 3 % per hour of storage for glycerol and sucrose stabilates respectively, and the loss rates were not significantly different. The results suggest that Chitongo stabilates and sucrose cryoprotected suspensions can be delivered on ice as is done for Katete. A graphical relationship of in vitro effective dose at 50 % infectivity (ED50) and in vivo protection rate was made. The relationship showed a 35 % loss of protection for a relatively low corresponding increase of ED50 from 0.006 to 0.007 tick equivalent.

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Immunisation against East Coast fever by the infection and treatment method: evaluation of the use of ice baths for field delivery and appraisal of an acid formulation of long-acting tetracycline

Cited by 13 publications

References 12 publications

Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo

Identification of hard ticks (Acari: Ixodidae) and seroprevalence to Theileria parva in cattle raised in North Kivu Province, Democratic Republic of Congo

The current status of major tick borne diseases in Zambia

Comparison of the survival on ice of thawed <i>Theileria parva</i> sporozoites of different stocks cryoprotected by glycerol or sucrose

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