Immunisation against East Coast fever: correlation between monoclonal antibody profiles of Theileria parva stocks and cross immunity in vivo

Irvin, A.D.; Dobbelaere, D. A. E.; Mwamachi, D.M.; Minami, Tetsuro; Spooner, P.R.; Ocama, J.G.R.

doi:10.1016/s0034-5288(18)32030-7

Cited by 115 publications

(31 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T h e transfer of meronts from infected to uninfected cells also has been reported to occur. (Radley et al, 1975c;Irvin et al, 1983;Morrison et al, 1986). This difference may explain why immunizing cattle against T. annulata using allogeneic-infected cells is easier than immunizing against T. parva.…”

Section: Theileria-infected Cells Are Not Yet Understood However Inmentioning

confidence: 99%

Theileria

Mehlhorn¹,

Schein²,

Ahmed³

1994

Parasitic Protozoa

View full text Add to dashboard Cite

Section: Theileria-infected Cells Are Not Yet Understood However Inmentioning

confidence: 99%

Theileria

Mehlhorn¹,

Schein²,

Ahmed³

1994

Parasitic Protozoa

View full text Add to dashboard Cite

“…Cell lines were established from PBMC by infection in vitro with sporozoites from tick salivary glands [16]. The sporozoites used were from the Muguga and Marikebuni stocks of T.parva which are known to differ on the basis of cross-protection [6]. Confirmation that cell lines were infected with particular parasite stocks was made by typing the intracellular schizont with parasite strain-specific mAb [17].…”

Section: T Pana-infected Cell Linesmentioning

confidence: 99%

“…Cross-protection experiments have shown that while immunization with the T. parva (Marikebuni) stock invariably protects against challenge with T. parva (Muguga) (Morzaria S. P., unpublished data), animals immunized with T. parva (Muguga) show variable degrees of susceptibility to challenge with T. parva (Marikebuni) [6]. Hence, if cell-mediated cytotoxic responses are important in protection, one might expect to find different degrees of parasite strain specificity of the cytotoxic effector cells generated in animals immunized with T. parva (Muguga).…”

Section: Previous Studies Have Demonstrated That Cells Infected Inmentioning

confidence: 99%

Generation of bovine cytotoxic cell lines, specific for cells infected with the protozoan parasite Theileria parva and restricted by products of the major histocompatibility complex

Goddeeris¹,

Morrison²,

Teale³

1986

Eur J Immunol

View full text Add to dashboard Cite

Cytotoxic cell lines specific for cells infected with the protozoan parasite Theileria parva were generated in vitro by repeated stimulation of peripheral blood mononuclear cells (PBMC) from immune animals with autologous parasitized cells from continuously growing cell lines. In PBMC of 5/7 immune animals tested, maximal levels of killing (80-100%) were obtained at effector to target ratios of 10/1 following 3 to 5 stimulations. No killing of uninfected lymphoblasts was observed. Cytotoxic activity was contained in the BoT4- lymphocyte population. The results of analyses of cytotoxicity on panels of target cells of diverse major histocompatibility (MHC) phenotypes indicated that the effectors were restricted by class I MHC antigens. Only autologous targets and allogenic targets sharing one or other bovine lymphocyte antigen A locus-encoded specificity with the cytotoxic cell line were killed. In addition, two of the three cytotoxic cell lines, when tested on targets infected with different parasite stocks, exhibited a high degree of specificity for the stock used for immunization and stimulation of the cultures. The capacity to generate highly cytotoxic, MHC-restricted effectors from peripheral blood was dependent on the immune status of the donor animals. This was confirmed by comparing cytotoxicity generated from the same animal before and after immunization using the same autologous infected cell line as a stimulator. These results indicate that these effector cells are analogous to the cytotoxic cells detected in vivo in cattle undergoing immunization or challenge with T. parva.

show abstract

“…Infection was characterized by a sustained fever, marked lymph node enlargement, severe lymphopenia and the animal was euthanized on day 14 post infection. C-1444 was infected by subcutaneous needle inoculation of 3 × 10 4 cryopreserved Ed64 Marikebuni (Irvin et al, 1983) strain parasites above the left pre-scapular lymph node and treated concurrently with 20 mg/kg long acting oxytetracycline (Biomycin 200). Due to the lower parasite dose and treatment with oxytetracycline, C-1444 showed no disease symptoms and no treatment was required.…”

mentioning

confidence: 99%