Detection of Theileria parva in tissues of cattle undergoing severe East Coast fever disease show significant parasite DNA accumulation in the spleen

Olds, Cassandra; Paul, Tasha; Scoles, Glen A.

doi:10.1016/j.vetpar.2016.11.012

Cited by 2 publications

(1 citation statement)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diagnostic testing involves finding piroplasms or schizonts using PCR, which has had a profound impact on the identification of tick-borne pathogens. PCR can identify Theileria in the blood of both carriers and clinical cases ( Table 1 ) [ 39 ]. Molecular diagnostics, however, are not widely available in comparison to serological tests even though they are more sensitive and could provide crucial insight in studying the role of carrier animals as a source of infection [ 40 , 41 ].…”

Section: Protozoal Agentsmentioning

confidence: 99%

Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle

Garcia

Weakley

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

Ticks and tick-borne diseases such as babesiosis, anaplasmosis, ehrlichiosis, Lyme disease, Crimean Congo hemorrhagic fever, and Rocky Mountain spotted fever pose a significant threat to animal and human health. Tick-borne diseases cause billions of dollars of losses to livestock farmers annually. These losses are partially attributed to the lack of sensitive, robust, cost effective and efficient diagnostic approaches that could detect the infectious pathogen at the early stages of illness. The modern nucleic acid-based multiplex diagnostic approaches have been developed in human medicine but are still absent in veterinary medicine. These powerful assays can screen 384 patient samples at one time, simultaneously detect numerous infectious pathogens in each test sample and provide the diagnostic answer in a few hours. Development, commercialization, and wide use of such high throughput multiplex molecular assays in the cattle tick-borne disease surveillance will help in early detection and control of infectious pathogens in the animal reservoir before community spread and spillover to humans. Such approaches in veterinary medicine will save animal life, prevent billions of dollars of economic loss to cattle herders and reduce unwanted stress to both human and animal health care systems. This literature review provides recent updates on molecular diagnostics of tick-borne pathogens and discusses the importance of modern nucleic acid high throughput multiplex diagnostic approaches in the prevention of tick-borne infection to livestock.

show abstract

Section: Protozoal Agentsmentioning

confidence: 99%

Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle

Garcia

Weakley

et al. 2022

Veterinary Sciences

View full text Add to dashboard Cite

show abstract

Molecular identification and characterization of piroplasm species in Hokkaido sika deer ( Cervus nippon yesoensis ), Japan

Elbaz

Moustafa

Lee

et al. 2017

Ticks and Tick-borne Diseases

View full text Add to dashboard Cite

Babesia and Theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesia and Theileria species by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87.7% (236/269) and 11.5% (31/269) of the examined PCR products hybridized with the probes which were designed to detect all Babesia and Theileria spp., all Theileria spp., all Babesia spp., Theileria sp. Thrivae and Babesia divergens-like, respectively. The 18S rRNA gene partial sequences were divided into Theileria sp. Thrivae, T. capreoli, B. divergens-like and an undescribed Babesia species. This study showed the first detection of the undescribed Babesia sp. from Japan. Therefore, more studies are required to understand the ecology of the newly detected tick-borne pathogens in Hokkaido.

show abstract

Detection of Theileria parva in tissues of cattle undergoing severe East Coast fever disease show significant parasite DNA accumulation in the spleen

Abstract: Graphical abstract

Cited by 2 publications

References 22 publications

Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle

Current and Future Molecular Diagnostics of Tick-Borne Diseases in Cattle

Molecular identification and characterization of piroplasm species in Hokkaido sika deer ( Cervus nippon yesoensis ), Japan

Contact Info

Product

Resources

About