Immune Responses of Mice Immunized with HBsAg Formulated in Naloxone/Alum Mixture: Comparison to Fendrix Vaccine

Nejati, Shima; Mirzaee, Sahar; Nouri, Hamid Reza; Farhoudi, Ramin; Namvar, Nabi Allah; Yazdi, Mohammad Hossein; Mahdavi, Mehdi

doi:10.5812/hepatmon.44536

Cited by 6 publications

(4 citation statements)

References 30 publications

(41 reference statements)

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“…HBsAg-specific serum antibody titers (IgG1, IgG, IgG2a) were estimated by adopting an indirect enzyme-linked immunosorbent assay (iELISA) on sera obtained from each experimental animal as described by Nejati et al . [ 24 ]. Briefly, 96-well ELISA plates (BRANDplates®, Germany) were coated with 100 µL of rHBsAg (from vaccine) in carbonate-bicarbonate buffer pH 9.5 (coating buffer), followed by overnight incubation at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

et al. 2023

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Background Despite the invasiveness of the Hepatitis B infection, its vaccines are only formulated with FDA-approved alum-based adjuvants, which poorly elicit a lasting immune response, hence the need for a more effective adjuvant system. This study evaluated the immunogenicity and toxicity of eggshell membranes (ESM) when administered as an adjuvant for the recombinant HBV vaccine (rHBsAg) in albino mice. Differential white blood cell analysis, as well as the titer measurement of Immunoglobulin G, subclass G1 and G2a on indirect ELISA, was performed to measure the immune-modulatory potentials of ESM. Moreover, analysis of the liver marker enzyme (AST and ALT) and body/liver weights was performed to ascertain the toxicity level of ESM. Finally, Immuno-informatic analysis was used to investigate the immune-modulatory potential of individual member proteins of ESM. Results Our results showed a significant improvement in the experimental group's lymphocyte count after boost-dose administration compared to the controls, whereas there was no significant change in the granulocyte population. Furthermore, the formulations (ESM-rHBsAg) significantly improved IgG and IgG1 titers after each successive immunization. Body/liver weight and liver function showed ESM non-toxic to mice. The immunoinformatic analysis discovered ovalbumin, lysozyme-C, and UFM-1 as the member proteins of ESM with immune-modulatory activities of activating antigen-presenting cells (APC). Conclusion This study has provided a clue into the potential valorization of eggshell membranes and their peptides as an adjuvant for vaccines such as HBV. We recommend more in-depth molecular analysis to support our findings as well as foster real-life application.

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Section: Methodsmentioning

confidence: 99%

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

et al. 2023

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“…Analysis of specific total antibodies (IgG) were performed using an optimized indirect ELISA method described by Nejati et al . [18] on a 96-well ELISA plates (BRANDplates®, Germany) coated with 100 μL of rHBsAg. Similarly, the specific IgG1 and IgG2a subclasses were performed using goat anti- mouse IgG1 and IgG2a secondary antibodies (Southern biotechnology, USA).…”

Section: Methodsmentioning

confidence: 99%

Experimental and Molecular Predictions of the Adjuvanticity of Snail Mucin on Hepatitis B Vaccine in Albino Mice

Joshua

Nwauzor

Odimegwu

et al. 2021

Preprint

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Although aluminum-containing adjuvants are widely used in human vaccination due to their excellent safety profile, they exhibit low effectiveness with many recombinant antigens. This study investigated the adjuvanticity of snail mucin with recombinant Hepatitis B Vaccine (rHBsAg). Twenty-five (25) female mice distributed unbiasedly into 5 groups were used in the study and were administered different rHBsAg/Mucin formulation at 7 days intervals. Blood samples were collected a day following the administration for analysis. The results of liver function and body weight analysis were indications that snail mucin had no adverse effect on the mice. The treatment group (administer mucin and rHBsAg) showed significantly (P<0.05) higher mean titres of anti-HBsAg antibodies when compared with the negative controls and the positive control administered with two doses of rHBsAg. Furthermore, a comparable immune response to positive control administered with three doses rHBaAG was recorded. In silico prediction, studies of the protein-protein interaction of a homology modelled snail mucus protein and HBsAg gave an indication of enhanced HBV antigen-antibody interaction. Therefore, this study has shown that snail mucin possesses some adjuvant properties and enhances immune response towards rHBsAg vaccine. However, there is a need for further molecular dynamics studies to understand its mechanism of action.

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“…Analysis of specific total antibodies (IgG) were performed using an optimized indirect ELISA method described by Nejati et al [16] on a 96-well ELISA plates (BRANDplates1, Germany) coated with 100 μL of rHBsAg. Similarly, the specific IgG1 and IgG2a subclasses were performed using goat anti-mouse IgG1 and IgG2a secondary antibodies (Southern biotechnology, USA).…”

Section: Measurement Of Antibody Titres Of Igg Igg1 and Igg2a Against Rhbsag Using Indirect Enzyme-linked Immunosorbent Assay (Ielisa)mentioning

confidence: 99%

Experimental and molecular predictions of the adjuvanticity of snail mucin on hepatitis B vaccine in albino mice

et al. 2021

View full text Add to dashboard Cite

Although aluminum-containing adjuvants are widely used in human vaccination due to their excellent safety profile, they exhibit low effectiveness with many recombinant antigens. This study investigated the adjuvanticity of snail mucin with recombinant Hepatitis B Vaccine (rHBsAg). Twenty-five (25) female mice distributed unbiasedly into 5 groups were used in the study and were administered different rHBsAg/Mucin formulation at 7 days intervals. Blood samples were collected a day following the administration for analysis. The results of liver function and body weight analysis were indications that snail mucin had no adverse effect on the mice. The treatment group (administer mucin and rHBsAg) showed significantly (P<0.05) higher mean titres of anti-HBsAg antibodies when compared with the negative controls and the positive control administered with two doses of rHBsAg after the boost doses (day 28). Furthermore, a comparable immune response to positive control administered with three doses rHBaAG was recorded. In silico prediction, studies of the protein-protein interaction of a homology modelled snail mucus protein and HBsAg gave an indication of enhanced HBV antigen-antibody interaction. Therefore, this study has shown that snail mucin possesses some adjuvant properties and enhances immune response towards rHBsAg vaccine. However, there is a need for further molecular dynamics studies to understand its mechanism of action.

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Immune Responses of Mice Immunized with HBsAg Formulated in Naloxone/Alum Mixture: Comparison to Fendrix Vaccine

Cited by 6 publications

References 30 publications

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

Experimental and Molecular Predictions of the Adjuvanticity of Snail Mucin on Hepatitis B Vaccine in Albino Mice

Experimental and molecular predictions of the adjuvanticity of snail mucin on hepatitis B vaccine in albino mice

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