Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein

Chen, Li-Hsuen; Kumanan, K.; Huang, Ching-Juo; McDonough, Sean P.; Stehman, Susan M.; Akey, Bruce; Huntley, John F.; Bannantine, John P.; Chang, Ching Hsun; Chang, Yung-Fu

doi:10.1016/j.vaccine.2007.12.014

Cited by 30 publications

(39 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. avium subsp. paratuberculosis 66115-98, a clinical isolate, was used to challenge the goats after immunization (28,29). This strain is IS900 positive and mycobactin dependent.…”

Section: Animalsmentioning

confidence: 99%

Evaluation of a Mycobacterium avium subsp. paratuberculosisleuDMutant as a Vaccine Candidate against Challenge in a Caprine Model

Faisal

Chen

Yan

et al. 2013

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

f Johne's disease (JD) is prevalent worldwide and has a significant impact on the global agricultural economy. In the present study, we evaluated the protective efficacy of a leuD (⌬leud) mutant and gained insight into differential immune responses after challenge with virulent M. avium subsp. paratuberculosis in a caprine colonization model. The immune response and protective efficacy were compared with those of the killed vaccine Mycopar. In vitro stimulation of peripheral blood mononuclear cells with johnin purified protein derivative showed that Mycopar and ⌬leuD generated similar levels of gamma interferon (IFN-␥) but significantly higher levels than unvaccinated and challenged phosphate-buffered saline controls. However, only with ⌬leuD was the IFN-␥ response maintained. Flow cytometric analysis showed that the increase in IFN-␥ correlated with proliferation and activation (increased expression of CD25) of CD4, CD8, and ␥␦T cells, but this response was significantly higher in ⌬leuD-vaccinated animals at some time points after challenge. Both Mycopar and ⌬leuD vaccines upregulated Th1/proinflammatory and Th17 cytokines and downregulated Th2/anti-inflammatory and regulatory cytokines at similar levels at almost all time points. However, significantly higher levels of IFN-␥ (at weeks 26 and 30), interleukin-2 (IL-2; week 18), IL-1b (weeks 14 and 22), IL-17 (weeks 18 and 22), and IL-23 (week 18) and a significantly lower level of IL-10 (weeks 14 and 18) and transforming growth factor ␤ (week 18) were detected in the ⌬leuD-vaccinated group. Most importantly, ⌬leuD elicited an immune response that significantly limited colonization of tissues compared to Mycopar upon challenge with wild-type M. avium subsp. paratuberculosis. In conclusion, the ⌬leuD mutant is a promising vaccine candidate for development of a live attenuated vaccine for JD in ruminants.

show abstract

“…M. avium subsp. paratuberculosis 66115-98, a clinical isolate, was used to challenge the goats after immunization (28,29). This strain is IS900 positive and mycobactin dependent.…”

Section: Animalsmentioning

confidence: 99%

Evaluation of a Mycobacterium avium subsp. paratuberculosisleuDMutant as a Vaccine Candidate against Challenge in a Caprine Model

Faisal

Chen

Yan

et al. 2013

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…High numbers of MAP organisms were detected in the liver, spleen To assess the protective efficacy of the inactivated experimental Johne's disease vaccine we have selected Balb/ C mice as per the proposed international guidelines on the choice of JD challenge/ infection models (Hines et al, 2007). Balb/C mice were also used to test the immunogenicity and protective efficacy of DNA vaccines and recombinant MAP proteins by several authors (Chen et al, 2008;Roupie et al, 2008). Liver, spleen and intestine samples were collected to determine the MAP burden in the vaccinated and sham control mice 4, 8 and 12 weeks after challenge.…”

Section: Real Time Pcr Quantification Of Map In Tissuesmentioning

confidence: 99%

“…F57 sequence being present in single copy in MAP genome, quantification of this gene in terms of copy number from the tissues allow us to predict the approximate number of MAP cells in each tissue directly. MAP load in the specific organs (liver, spleen and mesenteric lymph node) estimated by culture and histopathology lesion score are good indicators of infection status of mice following challenge with MAP (Veazey et al, 1995;Chen et al, 2008;Park et al, 2008) but are time taking. Kawaji et al (2007) reported that qPCR assay has similar sensitivity to a gold standard radiometric culture assay.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Mycobacterium avium subsp. paratuberculosis from the tissues of challenged mice using SYBR Green real time PCR assay for the assessment of vaccine efficacy

Chaitanya

Reddy

Raj

et al. 2018

IJAR

View full text Add to dashboard Cite

A SYBR Green real time PCR assay amplifying F57 gene of Mycobacterium avium subsp. paratuberculosis (MAP) was developed to evaluate the protective efficacy of the inactivated vaccine to prevent the colonization of MAP in the tissues of BALB/c mice challenge model. Bacterial burden in the liver, spleen and intestine of vaccinated and sham immunized control mice, after intra peritoneal challenge with MAP were quantified by real time PCR assay. A 195 bp fragment of MAP F57 sequence was cloned into TA cloning vector and the resultant recombinant plasmid was used to generate a series of quantification standards with copy number ranging from 10 9 to 1 copy. This absolute quantification technique is rapid and able to detect as few as 10 MAP organisms per gram of tissue and the assay has the efficiency of 0.982.

show abstract

“…The development of an efficacious subunit vaccine (marker vaccine) based on recombinant secretory proteins of MAP needs a combination of antigens appropriate for the broad range of varied MHC molecules present in an immune-competent outbred ruminant population (Derrick et al 2004;Cai et al 2005;Sable, Verma, Behera, et al 2005;Grover et al 2006). Immune responses to recombinant antigens of MAP have been studied and demonstrated the protective effects of recombinant MAP antigens in mice (Chen et al 2008) and calves ). In the latter study, protective efficacy of recombinant antigens was assessed for 85A, 85B, SOD and Map74F antigens in a goat model.…”

Section: Secreted Antigen Candidates For Marker Vaccine Developmentmentioning

confidence: 99%

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Chaubey

Gupta

et al. 2016

Veterinary Quarterly

View full text Add to dashboard Cite

Paratuberculosis (pTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP) in a wide variety of domestic and wild animals. Control of pTB is difficult due to the lack of sensitive, efficacious and cost-effective diagnostics and marker vaccines. Microscopy, culture, and PCR have been used for the screening of MAP infection in animals for quite a long time. Besides, giving variable sensitivity and specificity, these tests have not been considered ideal for large-scale screening of domestic livestock. Serological tests like ELISA easily detects anti-MAP antibodies. However, it cannot differentiate between the vaccinated and infected animals. Nanotechnology-based diagnostic tests are underway to improve the sensitivity and specificity. Newer generation diagnostic tests based on recombinant MAP secretory proteins would open new paradigm for the differentiation between infected and vaccinated animals and for early detection of the infection. Due to higher seroreactivity of secretory proteins vis-a-vis cellular proteins, the secretory proteins may be used as marker vaccine, which may aid in the control of pTB infection in animals. Secretory proteins can be potentially used to develop future diagnostics, surveillance and monitoring of the disease progression in animals and the marker vaccine for the control and eradication of pTB.

show abstract

Immune responses in mice to Mycobacterium avium subsp. paratuberculosis following vaccination with a novel 74F recombinant polyprotein

Cited by 30 publications

References 38 publications

Evaluation of a Mycobacterium avium subsp. paratuberculosisleuDMutant as a Vaccine Candidate against Challenge in a Caprine Model

Evaluation of a Mycobacterium avium subsp. paratuberculosisleuDMutant as a Vaccine Candidate against Challenge in a Caprine Model

Quantification of Mycobacterium avium subsp. paratuberculosis from the tissues of challenged mice using SYBR Green real time PCR assay for the assessment of vaccine efficacy

Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock

Contact Info

Product

Resources

About