Immune response to outer membrane antigens of Moraxella catarrhalis in children with otitis media

Faden, Howard; Hong, Jung Joo; Murphy, Timothy F.

doi:10.1128/iai.60.9.3824-3829.1992

Cited by 65 publications

(31 citation statements)

References 40 publications

(42 reference statements)

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“…All animals were challenged 5 weeks after the initial immunization with either the homologous or a heterologous strain. To each challenge group, untreated control animals were added (n=5, 5,6,6). Otomicroscopy was performed prior to immunization (day 0).…”

Section: Experimental Designmentioning

confidence: 99%

“…The targets for a protective immune response have not been identified, but the homogeneous composition of the outer membrane proteins and the lipopolysaccharide of M. cutarrhalis suggest that an antibody response to one strain may protect against others (1,24). However, the immune response to the organism seems to be poor, and young children fail to develop a systemic antibody response in a uniform manner after a middle ear infection (5,15).…”

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confidence: 99%

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Moraxella catarrhalis‐induced purulent otitis media in the rat middle earL

Westman

Melhus

Hellström

et al. 1999

APMIS

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To study the effects of viable and heat‐killed Moraxella catarrhalis bacteria on the middle ear mucosa and to evaluate the protection after whole‐cell immunizations, Sprague‐Dawley rats were challenged and rechallenged with four different M. catarrhalis strains. The animals were monitored by clinical observations, bacterial and histological samples from middle ears, and serum IgG levels. Only viable bacteria at a high concentration induced purulent otitis media, which was culture positive in 58% of the cases on day 4. The infection was characterized by a mild acute reaction lasting otomicroscopically about 8 days, together with quantitative and qualitative changes of the goblet cells. Structurally the mucosal effects of the heat‐killed bacteria were less pronounced in the early phase compared to the viable bacteria, but similar at the end of the experiment at 6 months. The intrabullar and subcutaneous immunizations evoked an IgG antibody response in all animals, and the protection rate after immunization was 50% or more. The induced protection was not strain‐specific. The study showed the rat to be a possible alternative for the study of different aspects of M. catarrhalis otitis media, an infection that is clinically and structurally different from that elicited by Streptococcus pneumoniae and Haentophilus influenzae in the rat.

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Section: Experimental Designmentioning

confidence: 99%

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confidence: 99%

Moraxella catarrhalis‐induced purulent otitis media in the rat middle earL

Westman

Melhus

Hellström

et al. 1999

APMIS

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“…cutarrhalis during infection have shown that approximately half the patients exhibit a response to antigens of this organism (2, 3, 4, 7,17). Young children with otitis media also failed to respond immunologically to M. catarrhalis in a uniform manner (8). Antibodies against M .…”

Section: Discussionmentioning

confidence: 99%

“…This finding may indicate that IgG3 is not protective in these patients who may then suffer repeated infections caused by M. catarrhalis. IgA and IgM responses were limited to a few patients in the present study. Previous studies have pointed to the lack of a regular response of these antibody classes (8,9). For the serological diagnosis of infections with M. catarrhalis the demonstration of the IgG3 response to outer membrane proteins appears to offer improved sensitivity as compared to the whole-cell ELI-SA.…”

Section: Discussionmentioning

confidence: 99%

Human immunoglobulin isotype and IgG subclass response to different antigens of Moraxella catarrhalis

Rahman

Holme

Jönsson

et al. 1997

APMIS

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Enzyme immunoassays were tested for the determination of antibodies of different isotypes and IgG subclasses to Moraxella catarrhalis in human sera. An assay based on an outer membrane protein preparation (OMP) as antigen was compared to assays using whole bacterial cells and a purified lipopolysaccharide preparation. There was a good correlation between the results obtained with the OMP preparation and the whole‐cell antigen. In paired sera, optimal sensitivity was obtained by using the OMP preparation as coating antigen and testing for a rise in IgG3 antibodies. However, patients with high levels of antibodies in acute serum had no or only an insignificant antibody response during infection.

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“…It is also associated with respiratory infection in older adults, especially exacerbations of chronic obstructive pulmonary disease (Black & Wilson, 1988;Sethi & Murphy, 2001). A rise in serum antibody against M. catarrhalis has been reported in these patients (Chapman et al, 1985;Black & Wilson, 1988;Faden et al, 1992;Christensen, 1999;Bakri et al, 2002). Thus, a vaccine that can reduce or prevent M. catarrhalis infection will benefit both young and old.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of the major surface epitope of the CopB outer membrane protein ofMoraxella catarrhalis

Liu

Xie

Mastri

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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The CopB outer membrane protein has been considered a vaccine candidate for the prevention of infections due to Moraxella catarrhalis. Monoclonal antibody 10F3 recognizes whole cells of about 70% of clinical isolates, suggesting that this epitope is reasonably conserved. To determine whether CopB has other surface epitopes, we analyzed M. catarrhalis isolates using polyclonal sera against recombinant CopB proteins from a 10F3 positive isolate and a 10F3 negative isolate, and polyclonal sera against synthetic peptides that contained the sequence corresponding to the 10F3 epitope region of three different isolates. Extensive cross-reactivity was observed with the anti-CopB sera towards purified recombinant CopB proteins in Western blot and antigen ELISA, implying that antigenic regions common to both proteins were present. However, anti-CopB sera resembled anti-CopB peptide sera in exhibiting similar binding specificity to whole cells, segregating M. catarrhalis isolates into four CopB groups. We subsequently cloned and sequenced the copB genes from representative isolates. The deduced CopB amino acid sequences and the degree of sequence identity also demonstrated the existence of the same four CopB groups. Each of the four groups had a unique sequence in the 10F3 epitope region and a fifth group had the epitope deleted. The polymorphism of the major surface epitope prompts further consideration regarding the utility of CopB as a vaccine component as well as the design of an efficacious CopB-based vaccine to achieve broad protection against Moraxella infection.

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Immune response to outer membrane antigens of Moraxella catarrhalis in children with otitis media

Cited by 65 publications

References 40 publications

Moraxella catarrhalis‐induced purulent otitis media in the rat middle earL

Moraxella catarrhalis‐induced purulent otitis media in the rat middle earL

Human immunoglobulin isotype and IgG subclass response to different antigens of Moraxella catarrhalis

Polymorphism of the major surface epitope of the CopB outer membrane protein ofMoraxella catarrhalis

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