Human immunoglobulin isotype and IgG subclass response to different antigens of Moraxella catarrhalis

Abstract

Enzyme immunoassays were tested for the determination of antibodies of different isotypes and IgG subclasses to Moraxella catarrhalis in human sera. An assay based on an outer membrane protein preparation (OMP) as antigen was compared to assays using whole bacterial cells and a purified lipopolysaccharide preparation. There was a good correlation between the results obtained with the OMP preparation and the whole‐cell antigen. In paired sera, optimal sensitivity was obtained by using the OMP preparation as coating antigen and testing for a rise in IgG3 antibodies. However, patients with high levels of antibodies in acute serum had no or only an insignificant antibody response during infection.

Synthesis and Characterization of Lipooligosaccharide-Based Conjugates as Vaccine Candidates for Moraxella ( Branhamella ) catarrhalis

“…This seems to correlate with a decrease in the incidence of bacteremia and otitis media caused by M. catarrhalis (5,13). Antibodies to whole cells, outer membrane proteins, and lipooligosaccharide (LOS) of M. catarrhalis have been detected in acute-and convalescentphase sera of adult patients (12,40,41). The rise of antibodies to the above-mentioned antigens in paired sera during infection was variable, with about 50% of patients having an increase and only a few having a significant increase (Ն4-fold).…”

New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates.

“…Bacterial surface antigens such as outer membrane proteins, [12][13][14][15] flagella 14) and lipopolysaccharides (LPS) 9,[12][13][14] are often purified to detect anti-bacterial cell surface antibodies. However, it can be unclear which antibody should be assayed for the detection of surface antigens that are related to immunity.…”