Immune response of channel catfish to ipopolysaccharide and whole cell Edward-siella ictaluri vaccines

Saeed, Mohsan; Plumb, John A.

doi:10.3354/dao002021

Cited by 56 publications

(44 citation statements)

References 4 publications

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“…Edwardsiella ictaluri bacterin was prepared by procedures described by Saeed & Plumb (1986). After 9 wk feeding, 120 fish treatment group were given a single (Guerin & Lovell m press).…”

Section: Methodsmentioning

confidence: 99%

Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds

Liu¹,

Plumb²,

Guerin³

et al. 1989

Dis. Aquat. Org.

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Channel catfish Ictalurus punctatus fingerlings in ponds were fed diets containing 0, 100, 500, 1000 or 4000 mg of vitamin C kg-' of diet for 9 wk. They were then immunized with EdwardsieUa jctaluri. One month after immunization, they were challenged with virulent E. ~ctaluri. Fish showed no clinical signs of vitamin C deficiency during the experimental period and final weights of the fish on the vanous diets were not significantly different ( p > 0.05). Increasing the level of vitamin C in the diet did not affect complement hemolytic activity and antibody titers. However, fish receiving vitamin C at 1000 mg kg-' showed increased resistance to E. ictaluri. These fish exhibited a 100-fold LDS0 compared to fish receiving a diet without supplemented vitamin C.

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“…Edwardsiella ictaluri bacterin was prepared by procedures described by Saeed & Plumb (1986). After 9 wk feeding, 120 fish treatment group were given a single (Guerin & Lovell m press).…”

Section: Methodsmentioning

confidence: 99%

Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds

Liu¹,

Plumb²,

Guerin³

et al. 1989

Dis. Aquat. Org.

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“…Later, the inflammatory response was evaluated by ink injection (Ellis et al, 1976), complete Freund's adjuvant and Staphylococcus aureus (Finn and Nielsen, 1971), carrageenin (Timur et al, 1977), Escherichia coli lipopolysaccharide (LPS), complete Freund's adjuvant, turpentine and carrageenin (White et al, 1981), Vibrio alginolyticus (MacArthur et al, 1984), liquid paraffin (Suzuki, 1986), Edwardsiella ictaluri LPS (Saeed and Plumb, 1986), Pseudomonas alcaligenes and Aeromonas punctata (Siwicki and Studnicka, 1987), Aeromonas salmonicida (Reite and Evensen, 1994), LPS (Brunetti et al, 1994), Freund's adjuvant and squalene (Jenkins and Klesius, 1998), microcystin (Vajcová et al, 1998), Escherichia coli , peptidoglican (Kono and Sakai, 2001), beta-glucan of Saccharomyces cerevisiae and Salmonella typhimurium LPS (Paulsen et al, 2001) and Mycobacterium marinum (Gauthier et al, 2003). In these studies, the inflammation was characterized by showing macrophages, leucocytes, fibroblasts and fibroplasia.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Martins

Myiazaki²,

Tavares‐Dias

et al. 2009

Braz. J. Biol.

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This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 µg carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish.Keywords: fish, hybrid tambacu, inflammation, exudate, carrageenin, thioglycollate. Caracterização da resposta inflamatória aguda no híbrido tambacu (Piaractus mesopotamicus macho × Colossoma macropomum fêmea) (Osteichthyes) ResumoEste estudo avaliou a resposta inflamatória aguda induzida por injeções de 0,5 mL de solução salina (controle), 500 µg de carragenina e 0,5 mL de tioglicolato a 3% na bexiga natatória de juvenis do híbrido tambacu. Os peixes foram distribuídos em três tratamentos, três repetições e aclimatados durante 10 dias antes do ensaio. A caracterização das células do exsudato inflamatório foi feita após coloração com Giemsa e PAS. Peixes injetados com carragenina apresentaram maior número de células no exsudato inflamatório do que com salina e tioglicolato. A porcentagem de trombócitos no exsudato foi maior nos injetados com carragenina quando comparada com a dos injetados com tioglicolato. Por outro lado, o percentual de granulócitos foi maior em animais injetados com tioglicolato do que em animais injetados com carragenina. A carragenina provocou maior migração de macrófagos para o foco inflamatório. O método de PAS Injection and collection of exudate cellsThe fish were anaesthetized by immersion in benzocaine solution (1 g.10 L -1 ) and injected with 500 µg carrageenin (Marine Colloids) dissolved in 0.5 mL sterile saline solution, 0.5 mL thioglycollate 3% and 0.5 mL sterile saline solution (control). After injection, the fish were maintained in the aquaria for 6 hours until the sacrifice. The interior of the swim bladder was washed with complete phosphate buffered saline containing 0.01 mL EDTA 5% and cell suspensions were centrifuged at 150 × gravity for 10 minutes before staining. The total number of cells was determined with a ha...

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“…Niejednokrotnie wykazano, że eksperymentalne podanie LPS u zwierząt doświadczalnych (myszy i szczurów), a także immunizacja pstrągów przeciwko jersiniozie i wrzodzienicy indukują stres oksydacyjny (1,3,32,33). Dotychczasowe badania u ryb wykazały silne działanie immunostymulujące lipopolisacharydu manifestujące się między innymi wzrostem aktywności metabolicznej komórek fagocytujących wskazujących na wystąpienie "wybuchu tlenowego" (10,25,28).…”

Section: Praca Oryginalnaunclassified

Effect of lipopolysaccharide (LPS) on the antioxidant enzyme system activity of erythrocytes and phagocytes of carps in in vivo studies

Sopińska¹,

Grochoła²

2018

Medycyna Weterynaryjna

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The purpose of this experiment was to estimate the activity of antioxidant enzymes (SOD, GPx, CAT) in erythrocytes and phagocytes of carps (Cyprinus carpio L.) after intraperitoneal injection of LPS at a dose of 75 μg/100 g b.w. Enzyme activity was determined 3, 6 and 12 hours, as well as 3, 7, 14 and 28 days after LPS administration. After 3 and 6 hours, SOD in erythrocytes increased, respectively, to 188% and 142% of its control group level, and after 12 hours, SOD activity was significantly reduced (117%) and remained unchanged until the end of the experiment. From 12 hours after LPS administration until the end of the study, the PGx level was statistically significantly lower than in the control group, whereas the catalase activity was statistically significantly lower than in the control group in all study periods. In kidney phagocytic cells, SOD activity after 12 hours and 3 days was similar to that in the control group, and in the following study periods it amounted to 66-78% of the control values. Until the 14th day of observation, PGx activity was statistically significantly lower than it was in the control group. Catalase activity in kidney leukocytes was statistically significantly lower than in the control group during the entire experiment, and the lowest in the first study days, amounting to 48-42% of the control group value. The results indicate a long-term decrease in antioxidant enzyme activities in the experimental fish (lasting 14 or 28 days)...

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Immune response of channel catfish to ipopolysaccharide and whole cell Edward-siella ictaluri vaccines

Cited by 56 publications

References 4 publications

Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds

Effect of megalevels of dietary vitamin C on the immune response of channel catfish Ictalurus punctatus in ponds

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Effect of lipopolysaccharide (LPS) on the antioxidant enzyme system activity of erythrocytes and phagocytes of carps in in vivo studies

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