Immune-related intestinal Cl- secretion. I. Effect of histamine on the T84 cell line

Abstract

The mast cell mediator, histamine, induces a rapid and transient increase in chloride secretion across monolayers of the human colonic epithelial cell line, T84. Threshold stimulation occurred at 3 X 10(-6) M histamine and a maximal effect at 10(-4) M. The effect was reproduced by the H1 agonists 2-methylhistamine and 2-pyridylethylamine, but not by the H2 agonists 4-methylhistamine and dimaprit, suggesting the involvement of an H1 receptor. Additionally, histamine's action was inhibited by an H1 antagonist, diphenhydramine, but not by an H2 antagonist, cimetidine. Histamine treatment increased free cytosolic calcium levels, but not those of adenosine 3',5'-cyclic monophosphate (cAMP) or guanosine 3',5'-cyclic monophosphate (cGMP). The mechanism of chloride secretion induced by histamine resembled that of carbachol, in that both 1) were associated with an increase in free cytosolic calcium, 2) had a site of activation at a basolaterally localized K+ channel, and 3) were potentiated by both cAMP- and cGMP-mediated secretagogues. These results suggest that histamine may act as an intestinal secretagogue via direct interactions with epithelial cells.

Mast cells and histamine contribute to bile acid-stimulated secretion in the mouse colon.

“…In previous studies, we showed that bile acids are able to activate mast cells (13). One likely mast cell-derived secretagogue is histamine, which is known to activate epithelial chloride secretion primarily via its actions on epithelial HI histamine receptors (31 ). Colonic tissue was pretreated with the Hi histamine receptor antagonists pyrilamine or DPH before stimulation with CDCA.…”

Concomitant activation of Cl‐ and K+ currents by secretory stimulation in human epithelial cells.

“…The transepithelial transport of Cl-is central to fluid Wasserman, Barrett, Huott, Beuerlein, Kagnoff & secretion across many epithelia including those of the Dharmsathaphorn, 1988;Dharmsathaphorn, Cohn & airways and of the intestine (Quinton, 1990;Halm & Beuerlein, 1989). The consensus view for active ClFrizzell, 1990;McCann & Welsh, 1990).…”

The Role of Histamine in the Mechanism of Antibiotic-Induced Changes in Colonic Ion and Water Transport

“…Цей ефект за механізмом схожий на реакцію карбахоліну і безпосередньо впли-ває підвищенням вмісту внутрішньоклітин-ного кальцію, активацією базолатеральних калієвих каналів і потенціюється цАМФ та цГМФ-залежними секретагогами [22]. У дослідженнях на ізольованій ділянці ди-стального відділу товстої кишки щурів, гі-стамін дозозалежно підвищував секреторний потенціал епітелію через вплив на хлорні і калієві канали апікальної та калієві канали базолатеральної мембран.…”