Immune Recovery Effects of Immunopotentiator from Pantoea agglomerans 1 (IP-PA1) on Low Antibody Productions in Response to Salmonella Enteritidis Vaccine and Sheep Red Blood Cells in Dexamethasone-Treated Stressed Chicken Models

Abstract

ABSTRACT. Considering the usefulness of the immunopotentiator from Pantoea agglomerans 1 (IP-PA1), which is a purified lipopolysaccharide (LPS) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. Three-week-old chickens daily administered 10 g/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relative thymic, bursal, and splenic weight losses; and decrease in the number of peripheral blood lymphocytes, as compared with the control chickens on day 35; the IP-PA1-pretreated, dexamethasone-treated chickens showed reduced weight losses. Five-to eight-week-old chickens administered 5 mg/kg of dexamethasone showed excessive apoptosis of thymic and bursal lymphocytes 24 hr after a single dexamethasone treatment; apoptosis was inhibited in the IP-PA1-pretreated, dexamethasone-treated chickens. Chickens daily administered 10 g/kg of dexamethasone for 35 days and injected with commercial Salmonella Enteritidis (SE) vaccine or sheep red blood cells (SRBC) on days 7 and 21 showed about 8-or 2-fold lower antibody production in response to SE or SRBC, respectively, as compared with the control chickens on day 35; the antibody production in response to SE or SRBC was increased in the IP-PA1-pretreated, dexamethasone-treated chickens. These results indicate that IP-PA1 exerts inhibitory effects on dexamethasone-induced immunosuppression and that it may be useful in controlling immunosuppression in poultry husbandry.

Impact of Prebiotics and Synbiotics Administered in ovo on the Immune Response against Experimental Antigens in Chicken Broilers

“…The SRBC antibody was determined by microagglutination (modified by Hebishima et al [30]). Serum samples taken on D7, D21, and D35 were inactivated by incubation at 56 • C for 30 min.…”

Eggshell Quality, Eggshell Structure and Small Intestinal Histology in Laying Hens Fed Dietary Pantoea-6®and Plant Extracts

“…As the nutritional composition of diets in all groups was almost the same, the tendency to better egg quality seems to have been induced by Pantoea-6 ® and plant extracts. In this study, Pantoea-6 ® was made from extraction of fermented wheat flour with Pantoea agglomerans, which is gram-negative bacteria (Hebishima et al, 2010). Rajput et al (2013) reported that LPS is a structural component of the outer membrane of gram-negative bacteria.…”

Effect of dexamethasone on bacteriostatic activity of turkey monocytes and implications for food safety

“…Hebishima et al (2010) reported that dexamethasone immunosuppression led to low antibody responses to a Salmonella Enteritidis vaccine in White Leghorn chickens suggesting that stressed chickens may have a higher risk of foodborne Fig. 3.…”