Immune reactivity of frozen-thawed murine spleen cells

Jennings, John J.; Yanari, S.S.; Reid, Robert H.

doi:10.1016/0011-2240(78)90037-8

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…There are several reports of succesful cryopreservation of human lymphocytes (Gross et al, 1975, Grant, 1976, Weiner, 1976, Birkeland, 1976 and lymphocytes of laboratory animals (Jennings et al, 1978) dog (Gluckman et al, 1977) and cattle (Kleinschuster et al, 1979, Tate andCram, 1982).…”

Section: Introductionmentioning

confidence: 99%

In vitro reactivity to concanavalin A of bovine lymphocytes after cryopreservation

Kuil

1984

Veterinary Immunology and Immunopathology

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Kuil. H., 1984. In vitro reactivity to concanavalin A of bovine lymphocytes after cryopreservation. Vet. Immuno!.Immunopathol., 7: 89-98.Cryopreservation of bovine peripheral lymphocytes and its effect on the in vitro response to concanavalin A tested in a microculture system is described. Using DMSO as cryoprotectant in the medium, the cells were cooled to -30°C at 1.3°C/minute and further to -80°C at 6°C/minute and then rapidly to -196°C by dropping in liquid nitrogen. The cells were recovered by rapid thawina in water at 30-35°C and washed twice before use in the stimulation test. Ten percent DMSO had a much better protective effect than 5%; addition of 25% fetal bovine serum to the freezing had no favourable effect. In most of the 16 animals used in the experiments the frozen lymphocytes gave the same or a higher response to Con A than those kept in the DMSO containing medium at 4°C for two hours. The responses of the frozen cells were comparable to those of fresh lymphocytes (kept at 4°C for two hours in medium without DMSO).

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