Abstract:Kuil. H., 1984. In vitro reactivity to concanavalin A of bovine lymphocytes after cryopreservation. Vet. Immuno!.Immunopathol., 7: 89-98.Cryopreservation of bovine peripheral lymphocytes and its effect on the in vitro response to concanavalin A tested in a microculture system is described. Using DMSO as cryoprotectant in the medium, the cells were cooled to -30°C at 1.3°C/minute and further to -80°C at 6°C/minute and then rapidly to -196°C by dropping in liquid nitrogen. The cells were recovered by rapid thawi… Show more
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