Immune reactivity in psoriatic Munro-Saboureau microabscesses, stratum corneum and blood vessels

Abstract

Background:A characteristic feature of early active psoriatic lesions is the intraepidermal penetration of neutrophils, with attendant formation of Munro-Saboureau microabscesses. Previous immunofluorescence studies have shown reactivity of in vivo binding of stratum corneum antibodies (SCAs) within the Munro-Saboreau microabscesses in cases of psoriasis.Aims:In our study, we aimed to investigate any correlation between the SCAs and the Munro-Saboureau microabscesses.Materials and Methods:We investigated 50 archival biopsies of psoriasis with Munro-Saboureau microabscesses, and attempted to confirm antibody colocalization within these microabcesses via immunohistochemistry staining. As controls, we utilized 50 skin biopsies from healthy patients undergoing esthetic plastic surgery procedures.Results:Within the Munro-Saboureau microabscesses, the following markers were statistically significantly positive relative to controls: CD1a, CD8, CD23, cyclooxygenase-2, myeloid histoid antigen, albumin, fibrinogen, kappa, lambda, von Willebrand factor, IgG, IgM, IgD, complement/C3c, C3d, myeloperoxidase, and carcinoembryonic antigen (P < 0.05). Autoreactivity to blood vessels was also detected, with multiple immunoglobulins and complement factors.Conclusions:We document important correlations between the Munro-Saboureau microabscesses, SCAs, and other immunoreactants.

IgD, cyclooxygenase-2 and ribosomal protein S6-PS240 immune response in a case of early psoriasis

“…We found expression of RIBO, showing differential phosphorylation in distinct epidermal layers; the expression was most pronounced in hyperproliferative regions. Based on our previous [2,3], and current findings and those of others, differential S6 phosphorylation may play a role in abnormal keratinocyte proliferation/differentiation in psoriasis, although only a few cases have been documented [2,3,5]. A larger series of cases is needed to adequately study the role of RIBO in intracellular pathways in psoriasis patients.…”

“…Lesional skin was biopsied and studied utilizing hematoxylin and eosin (H&E) staining, as well as via IHC. All techniques were performed as previously described [2,3].…”

“…IL-17-producing dermal γδ T cells seem to be recently linked to psoriasis [4]. In our case of early psoriasis, we investigated the presence of cyclooxygenase-2 (COX-2), and ribosomal protein S6-ps240 (RIBO) [2,3]. A 56 year old female presented to the dermatologist for a scaling, itching plaque.…”

“…The clinical presentation covers a spectrum including acute, chronic and relapsing forms; each stage seems to correlate with different immunologic markers. The chronic psoriasis immune response is characterized by hyperproliferative keratinocytes, infiltration of T cells, dendritic cells, macrophages and neutrophils, corneal ectopic expression of molecules and some autoreactivity [1][2][3][4]. In vivo binding of IgG in the stratum corneum of psoriatic lesions as well as in blood vessels has been previously documented [2,3] Autoantigen candidates for psoriasis include peroxiredoxin 2, ezrin, maspin, and heat shock protein 27.…”

“…The chronic psoriasis immune response is characterized by hyperproliferative keratinocytes, infiltration of T cells, dendritic cells, macrophages and neutrophils, corneal ectopic expression of molecules and some autoreactivity [1][2][3][4]. In vivo binding of IgG in the stratum corneum of psoriatic lesions as well as in blood vessels has been previously documented [2,3] Autoantigen candidates for psoriasis include peroxiredoxin 2, ezrin, maspin, and heat shock protein 27. Some putative amino acid sequences with homologies to streptococcal proteins have been shown to react with the sera from psoriasis patients [5].…”