Immune modulating effects of additional supplementation of estradiol combined with testosterone in murine testosterone-deficient NAFLD model

Okamura, Tomio; Hamaguchi, Masahide; Bamba, Ryo; Nakajima, Harushi; Yoshimura, Yuta; Kimura, Tomonori; Nishida, Kensuke; Fukuda, Takuya; Senmaru, Takafumi; Fukui, Michiaki

doi:10.1152/ajpgi.00310.2019

Cited by 23 publications

(24 citation statements)

References 31 publications

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“…The liver was perfused with 20 mL of PBS (pH 7.0) and then harvested. Isolation of hepatic lymphocytes by mechanical dissection was performed using the methods described in previous studies ( 11 ). Briefly, a suspension of liver tissue in Roswell Park Memorial Institute (RPMI) 1640 medium containing 20 mL/L fetal bovine serum (FBS, 2%) was centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…Stained cells were analyzed on a FACSCanto™ II system and the data were analyzed using FlowJo software version 10 (TreeStar, Ashland, OR, USA). The innate lymphoid cells were gated using methods provided in a previous study ( 11 , 12 ), with antibodies described in the supplementary file.…”

Section: Methodsmentioning

confidence: 99%

“…We used real-time reverse transcription polymerase chain reaction to quantify the mRNA expression levels of Tnfa, Il6, Il1b , Cd36 , Ccl2 , and Il22 using the same methods as in our previous study ( 11 ), the details for which are provided in the supplementary file. The primer sequence information was provided in the Supplementary Table .…”

Section: Methodsmentioning

confidence: 99%

“…Stained cells were analyzed using FACSCanto™ II system and the data were analyzed using FlowJo software version 10. Antibodies for gating of il-12- and il-1β -positive cells were the same as those used in our previous study ( 11 , 15 ), the details for which are provided in the supplementary file.…”

Section: Methodsmentioning

confidence: 99%

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Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance

et al. 2021

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Background and AimsMany nutritional and epidemiological studies have shown that high consumption of trans fatty acids can cause several adverse effects on human health, including cardiovascular disease, diabetes, and cancer. In the present study, we investigated the effect of trans fatty acids on innate immunity in the gut by observing mice fed with a diet high in trans fatty acids, which have been reported to cause dysbiosis.MethodsWe used C57BL6/J mice and fed them with normal diet (ND) or high-fat, high-sucrose diet (HFHSD) or high-trans fatty acid, high-sucrose diet (HTHSD) for 12 weeks. 16S rRNA gene sequencing was performed on the mice stool samples, in addition to flow cytometry, real-time PCR, and lipidomics analysis of the mice serum and liver samples. RAW264.7 cells were used for the in vitro studies.ResultsMice fed with HTHSD displayed significantly higher blood glucose levels and advanced fatty liver and intestinal inflammation, as compared to mice fed with HFHSD. Furthermore, compared to mice fed with HFHSD, mice fed with HTHSD displayed a significant elevation in the expression of CD36 in the small intestine, along with a reduction in the expression of IL-22. Furthermore, there was a significant increase in the populations of ILC1s and T-bet-positive ILC3s in the lamina propria in mice fed with HTHSD. Finally, the relative abundance of the family Desulfovibrionaceae, which belongs to the phylum Proteobacteria, was significantly higher in mice fed with HFHSD or HTHSD, than in mice fed with ND; between the HFHSD and HTHSD groups, the abundance was slightly higher in the HTHSD group.ConclusionsThis study revealed that compared to saturated fatty acid intake, trans fatty acid intake significantly exacerbated metabolic diseases such as diabetes and fatty liver.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance

et al. 2021

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“…Each fatty acid methyl ester was detected in selected ion monitoring mode. All results were normalized to the peak height of the internal standard C17:0 [23]. Average CV was 0.0245% (range 0.004-0.039%) (n = 3) for intra-day precision during a single analytical run and average CV 0.00237% (0.0010-0.0033%) on two separated days (n = 6) for inter-day precision.…”

Section: Measurement Of Circulating Free Fatty Acidsmentioning

confidence: 99%

Low circulating dihomo-gamma-linolenic acid is associated with diabetic retinopathy: a cross sectional study of KAMOGAWA-DM cohort study

et al. 2021

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Extracellular lipidome change by an SGLT2 inhibitor, luseogliflozin, contributes to prevent skeletal muscle atrophy in db/db mice

Bamba

Okamura

Hashimoto

et al. 2021

J cachexia sarcopenia muscle

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Background Diabetes mellitus increases the excretion of urinary glucose from the renal glomeruli due to elevated blood glucose levels. In the renal tubules, SGLT2 is expressed and reabsorbs the excreted urinary glucose. In the pathogenesis of diabetes mellitus, glucose reabsorption by SGLT2 is increased, and SGLT2 inhibitors improve hyperglycaemia by inhibiting this reabsorption. When urinary glucose excretion is enhanced, glucose supply to skeletal muscle may be insufficient and muscle protein catabolism may be accelerated. On the other hand, SGLT2 inhibitors not only ameliorate hyperglycaemia but also improve fatty acid metabolism in muscle, which may prevent muscle atrophy. Methods Eight-week-old male db/m mice or db/db mice were fed a standard diet with or without the SGLT2i luseogliflozin (0.01% w/w in chow) for 8 weeks. Mice were sacrificed at 16 weeks of age, and skeletal muscle and serum lipidomes, as well as skeletal muscle transcriptome, were analysed. Results Administration of SGLT2i led to not only decreased visceral fat accumulation (P = 0.004) but also increased soleus muscle weight (P = 0.010) and grip strength (P = 0.0001). The levels of saturated fatty acids, especially palmitic acid, decreased in both muscles (P = 0.017) and sera (P = 0.041) upon administration of SGLT2i, while the content of monosaturated fatty acids, especially oleic acid, increased in both muscle (P < 0.0001) and sera (P = 0.009). Finally, the accumulation of transcripts associated with fatty acid metabolism, such as Scd1, Fasn, and Elovl6, and of muscle atrophy-associated transcripts, such as Foxo1, Mstn, Trim63, and Fbxo32, decreased following SGLT2i administration. Conclusions Intramuscular fatty acid metabolism and gene expression were influenced by the extracellular lipidome, which was modified by SGLT2i. Hence, secondary effects, other than the hypoglycaemic effects of SGLT2i, might lead to the alleviation of sarcopenia.

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Immune modulating effects of additional supplementation of estradiol combined with testosterone in murine testosterone-deficient NAFLD model

Cited by 23 publications

References 31 publications

Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance

Trans Fatty Acid Intake Induces Intestinal Inflammation and Impaired Glucose Tolerance

Low circulating dihomo-gamma-linolenic acid is associated with diabetic retinopathy: a cross sectional study of KAMOGAWA-DM cohort study

Extracellular lipidome change by an SGLT2 inhibitor, luseogliflozin, contributes to prevent skeletal muscle atrophy in db/db mice

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